Art. -Nr.: HY-N2148

Cytosporone B

Name: Cytosporone B
Brand: MedChemExpress
SKU: HY-N2148
Price: 50.00 USD
Availability: InStock
Rating: 5 (18 reviews)

(Synonyms: Csn-B; Dothiorelone G)

18 Cited Publications

Kundenbewertung

Based on 18 publication(s) in Google Scholar

Cytosporone B (Csn-B; Dothiorelone G) is a naturally occurring nuclear orphan receptor Nur77/NR4A1 agonist with an EC₅₀ of 0.278 nM.

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Reinheit : 98.80%
CAS. Nr.: 321661-62-5
Formel: C₁₈H₂₆O₅
Molecular Weight:322.40

Speicherung:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 1 year , -20°C, 6 months

Cytosporone B Chemical Structure

CAS. Nr.: 321661-62-5

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Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biologische Aktivität
- Beschreibung
- IC₅₀ & Target
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Publications (18)
Lösungsmittel & Löslichkeit
- In Vitro
- In Vivo
Reinheit & Dokumentation
Verweise
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

Cytosporone B (Csn-B; Dothiorelone G) is a naturally occurring nuclear orphan receptor Nur77/NR4A1 agonist with an EC₅₀ of 0.278 nM.

IC₅₀ & Target

Nur77/NR4A1

Alle Nuclear Hormone Receptor 4A/NR4A Isoform-spezifische Produkte anzeigen :

Cellular Effect

Cell Line	Type	Value	Description	References
BGC-823	EC₅₀	1.15 x 10^-10 M Compound: 1, Csn-B	Agonist activity at nuclear orphan receptor Nur77 expressed in human BGC823 cells co-transfected with fused GAL4-LBD assessed as transactivation after 12 hrs by luciferase reporter gene assay Agonist activity at nuclear orphan receptor Nur77 expressed in human BGC823 cells co-transfected with fused GAL4-LBD assessed as transactivation after 12 hrs by luciferase reporter gene assay	[PMID: 18690216]
BGC-823	EC₅₀	2.78 x 10^-10 M Compound: 1, Csn-B	Agonist activity at nuclear orphan receptor Nur77 expressed in human BGC823 cells co-transfected with fused GAL4-Nur77 assessed as transactivation after 12 hrs by luciferase reporter gene assay Agonist activity at nuclear orphan receptor Nur77 expressed in human BGC823 cells co-transfected with fused GAL4-Nur77 assessed as transactivation after 12 hrs by luciferase reporter gene assay	[PMID: 18690216]
LNCaP	IC₅₀	13.4 μM Compound: 1, Csn-B	Cytotoxicity against human LNCAP cells after 72 hrs by MTT assay Cytotoxicity against human LNCAP cells after 72 hrs by MTT assay	[PMID: 24795803]
NCI-H460	IC₅₀	15.3 μM Compound: 1, Csn-B	Cytotoxicity against human H460 cells after 72 hrs by MTT assay Cytotoxicity against human H460 cells after 72 hrs by MTT assay	[PMID: 24795803]

In Vitro

Cytosporone B targets the ligand binding domain of Nur77, which selectively stimulates the transactivational activity of Nur77. Cytosporone B induces luciferase activity in cells that are cotransfected with GAL4- Nur77 or GAL4-LBD. The EC₅₀ of cytosporone B for Nur77 is 0.278 nM. Cytosporone B displays robust pro-apoptotic activity in gastric cancer cells BGC-823. 63.5% of the cells are apoptotic when treated with cytosporone B for 48 h. Cytosporone B shows selective effect on cancerous cells. Cytosporone B inhibits proliferation of human gastric cancer BGC-823 cells and human colon cancer SW620 cells by 470%, but it has a modest effect on human lung cancer H1299 cells and human hepatoma HepG2 cells^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. .

In Vivo

In the hepatocytes of wild-type mice, the transcriptional activity of the reporter is induced five-fold with cytosporone B treatment. In wild-type mice, cytosporone B treatment significantly increases glucose levels from 3.2 to 11.4 mM within the first 30 min, and thereafter blood glucose gradually decreased before reaching the initial level after 300 min^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS. Nr. 321661-62-5
Appearance Solid
Molecular Weight 322.40
Formel C₁₈H₂₆O₅
Color Off-white to light yellow
SMILES

O=C(OCC)CC1=CC(O)=CC(O)=C1C(CCCCCCC)=O
Synonyms

Csn-B; Dothiorelone G
Structure Classification
- Phenols
- Polyphenols
Initial Source
- Microorganisms
fungus?Eupenicillium?sp. HJ002
Versand

Room temperature in continental US; may vary elsewhere.

Speicherung

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	1 year
	-20°C	6 months

Publications (18)

All Cell Proliferation/Viability AssayIn Vivo Efficacy StudyWBBio/Physico-chemical Assay

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Journal Impact Factor

Chem Eng J

2025 Aug 24.

Cytosporone B purchased from MedChemExpress. Usage Cited in: Chem Eng J. 2025 Aug 24.

The viabilities of RAW264.7 cells and RSC96 cells after 24 h treatment with varying concentrations of Cytosporone B (CSNB, 4-8 μM) and SAL, respectively.
Cell Death Dis

PARL stabilizes mitochondrial BCL-2 via Nur77-mediated scaffolding as a therapeutic strategy for Parkinson's disease. [Abstract]

2025 Oct 6;16(1):700. PMID: 41052999
Int J Biol Macromol

High throughput screening of nuclear receptors identifies NR4A1 as a novel tumor suppressor in gallbladder cancer. [Abstract]

2026 Jun 11:373:152939. PMID: 42276489
Clin Transl Med

NR4A1 suppresses pyroptosis by transcriptionally inhibiting NLRP3 and IL-1β and co-localizing with NLRP3 in trans-Golgi to alleviate pathogenic bacteria-induced colitis. [Abstract]

2021 Dec;11(12):e639. PMID: 34923769
Cell Rep

A Regulation Loop between YAP and NR4A1 Balances Cell Proliferation and Apoptosis. [Abstract]

2020 Oct 20;33(3):108284. PMID: 33086070

Cytosporone B purchased from MedChemExpress. Usage Cited in: Cell Rep. 2020 Oct 20;33(3):108284. [Abstract]

Tumor weight, and tumor images of nude mice with subcutaneous injection of HeLa cells and then intraperitoneal injection DMSO and 10 mg/kg Cytosporone B (CsnB). The results showed that Cytosporone B (CsnB) inhibited tumor growth in wild-type (WT) mice, but this effect was not observed in NR4A1-deficient mice.
Pharm Biol

Pterostilbene pre-treatment reduces LPS-induced acute lung injury through activating NR4A1. [Abstract]

2022 Dec;60(1):394-403. PMID: 35271397
Int Immunopharmacol

Clemastine fumarate alleviates endoplasmic reticulum stress through the Nur77/GFPT2/CHOP pathway after ischemia/reperfusion in rat hearts. [Abstract]

2025 Mar 6:149:114242. PMID: 39929094
J Thromb Haemost

Cytosporone B ameliorates hypercoagulability in sepsis by agonizing the Nur77-thrombomodulin pathway. [Abstract]

2025 Oct 31:S1538-7836(25)00673-7. PMID: 41177456

Cytosporone B purchased from MedChemExpress. Usage Cited in: J Thromb Haemost. 2025 Oct 31:S1538-7836(25)00673-7. [Abstract]

Cytosporone B (Csn-B; 5-10 μM; 1-48 h) could upregulate Nur77 expression in HUVECs in a dose- and time-dependent manner.

Cytosporone B purchased from MedChemExpress. Usage Cited in: J Thromb Haemost. 2025 Oct 31:S1538-7836(25)00673-7. [Abstract]

After transfection with siCTL or siNur77 for 48 hours, HUVECs were treated with vehicle or Cytosporone B (Csn-B) (10 μM) for 24 hours. Cells were then treated with TNF-α (100 ng/mL) for 2 hours. The results showed that Cytosporone B (Csn-B) significantly prolonged the clotting time of HUVECs stimulated by TNF-α, but this effect was abolished when Nur77 was knocked down.

Cytosporone B purchased from MedChemExpress. Usage Cited in: J Thromb Haemost. 2025 Oct 31:S1538-7836(25)00673-7. [Abstract]

Cytosporone B (Csn‑B, 13 mg/kg; i.p.; once daily for 3 days) improved the 72‑hour survival rate of mice following cecal ligation and puncture (CLP) surgery. At 5 hours after CLP, Csn‑B attenuated the shortening of tail bleeding time and prothrombin time (PT), as well as the decrease in platelet count in mice.
Eur J Immunol

Nur77 Is Associated With Polyfunctional Properties in Virus-Specific Human CD8+ T Cells. [Abstract]

2026 May;56(5):e70202. PMID: 42117214
Clin Transl Immunology

SARS-CoV-2-infected human airway epithelial cell cultures uniquely lack interferon and immediate early gene responses caused by other coronaviruses. [Abstract]

2024 Apr 15;13(4):e1503. PMID: 38623540
Biol Reprod

NR4A1-mediated regulation of lipid droplets in progesterone synthesis in goat luteal cells†. [Abstract]

2024 Sep 14;111(3):640-654. PMID: 38936833
J Cardiovasc Transl Res

NR4A1 Aggravates Myocardial Ischaemia-Reperfusion Injury by Inhibiting OPA1-Mediated Mitochondrial Fusion. [Abstract]

2023 Oct;16(5):1050-1063. PMID: 37249897
Chinese J Anal Chem

17 September 202.
Cell Press Blue

2026 Apr 22.
Mi-JHM

2025 May 8.
SSRN

2025 Feb 6.
medRxiv

2024 Mar 15.
bioRxiv

2024 Feb 8.

Lösungsmittel & Löslichkeit

In Vitro:

DMSO : 100 mg/mL (310.17 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Ethanol : ≥ 50 mg/mL (155.09 mM)

* "≥" means soluble, but saturation unknown.

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

3.1017 mL

15.5087 mL

31.0174 mL

5 mM

0.6203 mL

3.1017 mL

6.2035 mL

10 mM

0.3102 mL

1.5509 mL

3.1017 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (7.75 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.5 mg/mL (7.75 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (274 KB)
SDS (392 KB)
- English - EN (392 KB)
- Français - FR (392 KB)
- Deutsch - DE (392 KB)
- Norwegian - NO (392 KB)
- Español - ES (392 KB)
- Swedish - SV (392 KB)
- Italian - IT (392 KB)
- Korean - KR (392 KB)
- Portuguese - PT (392 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. Zhan Y, et al. Cytosporone B is an agonist for nuclear orphan receptor Nur77. Nat Chem Biol. 2008 Sep;4(9):548-56. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
Ethanol / DMSO	1 mM	3.1017 mL	15.5087 mL	31.0174 mL	77.5434 mL
	5 mM	0.6203 mL	3.1017 mL	6.2035 mL	15.5087 mL
	10 mM	0.3102 mL	1.5509 mL	3.1017 mL	7.7543 mL
	15 mM	0.2068 mL	1.0339 mL	2.0678 mL	5.1696 mL
	20 mM	0.1551 mL	0.7754 mL	1.5509 mL	3.8772 mL
	25 mM	0.1241 mL	0.6203 mL	1.2407 mL	3.1017 mL
	30 mM	0.1034 mL	0.5170 mL	1.0339 mL	2.5848 mL
	40 mM	0.0775 mL	0.3877 mL	0.7754 mL	1.9386 mL
	50 mM	0.0620 mL	0.3102 mL	0.6203 mL	1.5509 mL
	60 mM	0.0517 mL	0.2585 mL	0.5170 mL	1.2924 mL
	80 mM	0.0388 mL	0.1939 mL	0.3877 mL	0.9693 mL
	100 mM	0.0310 mL	0.1551 mL	0.3102 mL	0.7754 mL

Bioz

Keywords

Cytosporone B

18 Cited Publications

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Cytosporone B

Publications Citing Use of MedChemExpress (MCE) Cytosporone B

Alle Nuclear Hormone Receptor 4A/NR4A Isoform-spezifische Produkte anzeigen