Bafilomycin D
Based on 1 Customer Validation
Bafilomycin D is a specific inhibitor of vacuolar-type ATPase (V-ATPase). Bafilomycin D has antimicrobial, insecticidal, herbicidal and cytotoxic activity.
Nur für Forschungszwecke. Wir verkaufen nicht an Patienten.
- Reinheit : 98.4%
- CAS. Nr.: 98813-13-9
- Formel: C35H56O8
- Molecular Weight:604.81
-
Speicherung:Powder -20°C, 3 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biologische Aktivität
-
Chemical Information
-
Lösungsmittel & Löslichkeit
- Protokoll
- Reinheit & Dokumentation
- Verweise
-
Help & FAQs
-
HY-100558
Bafilomycin A1V-ATPase Inhibitor -
HY-130173
Bafilomycin C1Antibiotic -
HY-13433
ThapsigarginER Stress Inducer -
HY-N6782
OligomycinH+-ATP-Synthase Inhibitor -
HY-16589
Oligomycin AMitochondrial ATP Synthase Inhibitor -
HY-N6778
PaxillineBK Channel Blocker -
HY-N6771
Cyclopiazonic acidECAs Inhibitor -
HY-Y0413
Biacetyl monoximeATPase Inhibitor -
HY-15417
ML-7 hydrochlorideMLCK Inhibitor -
HY-Y0292
Lithium carbonateBiochemical Reagent -
HY-15718A
Istaroxime hydrochlorideCalcium Channel Activator
Biologische Aktivität
Beschreibung
Chemical Information
-
CAS. Nr. 98813-13-9
-
Appearance Solid
-
Molecular Weight 604.81
-
Formel C35H56O8
-
Color Off-white to light yellow
-
SMILES
O=C1O[C@H]([C@@H](C)[C@@H](O)[C@H](C)C(/C=C/[C@H](C)[C@H](O)C(C)C)=O)[C@@H](OC)/C=C/C=C(C)/C[C@H](C)[C@H](O)[C@H](C)/C=C(C)/C=C1\OC
-
Structure Classification
-
Initial Source
Streptomyces varsoviensis
-
Versand
Room temperature in continental US; may vary elsewhere.
-
Speicherung
Powder -20°C 3 years In solvent -80°C 6 months -20°C 1 month
Lösungsmittel & Löslichkeit
In Vitro:
DMSO : 50 mg/mL (82.67 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Methanol : 6.1 mg/mL (10.09 mM; Need ultrasonic and warming)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Konzentration (Stammlösung) × Volumen (Stammlösung) = Konzentration (Ziellösung) × Volumen (Ziellösung)
Protokoll
-
Research Protocol for Infectious Diseases
Infectious-disease experiments test how pathogens interact with host barriers, innate immune receptors, inflammatory signaling, pathogen replication, and tissue injury; pattern-recognition receptors such as TLRs, RIG-I-like receptors, NOD-like receptors, and inflammasomes detect microbial molecules and activate NF-κB, interferon, and cytokine responses. The central hypothesis is that infection severity reflects the balance between pathogen burden and host response: protective inflammation restricts pathogen growth, whereas excessive or mislocalized inflammation contributes to tissue damage and disease phenotype. Unresolved questions include which host pathways are protective versus pathogenic, why some infection models fail to translate to human disease, and which combined readouts best predict clinically relevant infection outcomes.
-
Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.
-
Cell Viability Determination by MTT Colorimetric Assay
The following protocol uses the MTT colorimetric assay as a classic literature-established method for assessing cell viability/metabolic activity in cultured mammalian cells. MTT[3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] is reduced by metabolically active cells to a colored formazan product; the amount of formazan is quantified spectrophotometrically and provides an indirect measure of metabolically active viable cells. Importantly, MTT reduction reflects cellular oxidoreductase/metabolic activity rather than an absolute direct count of living cells, so changes in cellular metabolism can alter the signal independently of cell number.
Reinheit & Dokumentation
-
Data Sheet (268 KB)
-
SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
-
Handling Instructions (2659 KB)
Verweise
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| Methanol / DMSO | 1 mM | 1.6534 mL | 8.2671 mL | 16.5341 mL | 41.3353 mL |
| 5 mM | 0.3307 mL | 1.6534 mL | 3.3068 mL | 8.2671 mL | |
| 10 mM | 0.1653 mL | 0.8267 mL | 1.6534 mL | 4.1335 mL | |
| DMSO | 15 mM | 0.1102 mL | 0.5511 mL | 1.1023 mL | 2.7557 mL |
| 20 mM | 0.0827 mL | 0.4134 mL | 0.8267 mL | 2.0668 mL | |
| 25 mM | 0.0661 mL | 0.3307 mL | 0.6614 mL | 1.6534 mL | |
| 30 mM | 0.0551 mL | 0.2756 mL | 0.5511 mL | 1.3778 mL | |
| 40 mM | 0.0413 mL | 0.2067 mL | 0.4134 mL | 1.0334 mL | |
| 50 mM | 0.0331 mL | 0.1653 mL | 0.3307 mL | 0.8267 mL | |
| 60 mM | 0.0276 mL | 0.1378 mL | 0.2756 mL | 0.6889 mL | |
| 80 mM | 0.0207 mL | 0.1033 mL | 0.2067 mL | 0.5167 mL |
Bafilomycin D Related Classifications
HY-108970 Related Classifications
Powered by Bioz