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Art. -Nr.: HY-D3179

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CDg13

CDg13 is a fluorescent probe that specifically detects living neural stem/progenitor cells. CDg13 localizes to the endoplasmic reticulum via its dihexyl moiety, with no interaction with endoplasmic reticulum biomolecules. CDg13 undergoes selective efflux by active ABCG2 transporters, with increased intracellular accumulation following ABCG2 inhibition or knockdown. CDg13 isolates and enriches self-renewable neural stem/progenitor cells from embryonic mouse brain tissue, stains neural stem/progenitor cells brightly, and sorts ABCG2_low cell populations from heterogeneous populations. (Ex/Em = 520/553 nm)

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CAS. Nr.: 1451048-96-6
Formel: C₃₂H₃₅Cl₂NO₄
Molecular Weight:568.53

Speicherung:

Please store the product under the recommended conditions in the Certificate of Analysis.

CDg13 Chemical Structure

CAS. Nr.: 1451048-96-6

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Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biologische Aktivität
- Beschreibung
- In Vitro
Chemical Information
Reinheit & Dokumentation
Verweise
Calculators
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

In Vitro

CDg13 (1 μM; 1 h) selectively stains live mouse NS5 (NSPC) cells with bright fluorescence, while showing minimal staining of MEF, mESC, and differentiated NS5 cells^[1].
CDg13 (1 μM; 1 h) fluorescence in D-NS5 cells is inversely correlated with Abcg2 expression, as demonstrated by enhanced fluorescence in Abcg2-knockdown cells stained with it^[1].
CDg13 (1-10 μM; 48 h-7days) has very low toxicity to live mouse NSPCs^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Immunofluorescence^[1]

Cell Line:

mouse embryonic fibroblasts (MEF), mouse embryonic stem cells (mESC), mouse NSPC line (NS5), differentiated NS5 (D-NS5)
Concentration:

1 μM
Incubation Time:

1 h
Result:

Showed bright fluorescence in NS5 cells, with significantly lower fluorescence in MEF, mESC, and D-NS5 cells, as visualized by fluorescence microscopy and confirmed by flow cytometry histograms.

Cell Cytotoxicity Assay^[1]

Cell Line:

mouse NSPCs (NS5 cells)
Concentration:

1, 10 μM
Incubation Time:

48 h; 7 days;
Result:

Showed no cell toxicity after incubation with 10 μM for 48 h.
Showed no effect on neurosphere size after incubation with 1 μM for 7 days.

Chemical Information

CAS. Nr. 1451048-96-6
Molecular Weight 568.53
Formel C₃₂H₃₅Cl₂NO₄
SMILES

CCCCCCN(C(C1=CC=C(C=C1)C2=C3C=C(C(C=C3OC4=C2C=C(C(O)=C4)Cl)=O)Cl)=O)CCCCCC
Versand

Room temperature in continental US; may vary elsewhere.
Speicherung

Please store the product under the recommended conditions in the Certificate of Analysis.

Reinheit & Dokumentation

Handling Instructions (2659 KB)

Verweise

[1]. Kim B, et al. A Fluorescent Probe for Neural Stem/Progenitor Cells with High Differentiation Capability into Neurons. Chembiochem. 2016;17(22):2118-2122. [Content Brief]

Calculators

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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100 mg

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