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Green CMFDA

(Synonyms: CMFDA)
15 Cited Publications
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Based on 15 publication(s) in Google Scholar

Green CMFDA is a cell-permeable fluorescent probe with Em of 514 nm and Ex of 485 nm and can be used as a cell tracer. Green CMFDA can be cleaved by non-specific esterases common in living cells, producing a fluorescent compound, fluorescein, visible using a fluorescent microscope.

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  • Reinheit : 99.42%
  • CAS. Nr.: 136832-63-8
  • Formel: C25H17ClO7
  • Molecular Weight:464.85
  • Speicherung:

    4°C, protect from light

    * In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

  • Green CMFDA Chemical Structure
    Green CMFDA Chemical Structure
  • Experimental Validation Results for Green CMFDA
  • Experimental Validation Results for Green CMFDA
  • Experimental Validation Results for Green CMFDA
  • Experimental Validation Results for Green CMFDA
  • Experimental Validation Results for Green CMFDA
  • Experimental Validation Results for Green CMFDA
CAS. Nr.: 136832-63-8
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Publications Citing Use of MedChemExpress (MCE) Green CMFDA

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IFCell Imaging/StainingFlow Cytometry
  • Cell Imaging/Staining
  • Cell Imaging/Staining
  • IF
  • Flow Cytometry
  • Cell Imaging/Staining
  • Flow Cytometry

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  • Green CMFDA Chemical Structure
    Green CMFDA Chemical Structure
    1/16
  • 1/16

    Green CMFDA purchased from MedChemExpress. Usage Cited in: Eur J Pharmacol. 2026 Jan 12:1011:178432. [Abstract]

    Customer Validation
     Representative images of 143B cell invasion at 0 and 24 h, following exposure to robinetin (A2) or robinetin/doxorubicin (Dox) combinations. MRC-5 lung cells were stained with Cell TrackerTM Red CMTPX dye and 143B osteosarcoma cells with Cell TrackerTM Green CMFDA (10 μM; 15 min) dye.

  • 1/16

    Green CMFDA purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2025 Jan 14;16(1):17. [Abstract]

    Customer Validation
     A high-resolution live-cell imaging was performed to follow up the activity of Jurkat T cells co-cultured with AGS cells overexpressing CagA and SQLE; living tumor cells: red (DiD perchlorate); living Jurkat T cells: green (Green CMFDA). Scale bar = 100 μm.

  • 1/16

    Green CMFDA purchased from MedChemExpress. Usage Cited in: J Neuroinflammation. 2025 Mar 19;22(1):88. [Abstract]

    Customer Validation
     Representative immunofluorescence images of Green CMFDA (30 min) (green) and Iba1 (red) in microglia after 10µM Hb and ACs stimulation for 12 h. Nuclei were counterstained with DAPI (blue). Scale bar, 50 μm.

  • 1/16

    Green CMFDA purchased from MedChemExpress. Usage Cited in: J Neuroinflammation. 2025 Mar 19;22(1):88. [Abstract]

    Customer Validation
     Flow cytometry analysis and quantification of Green CMFDA (30 min)+Iba1+ microglia (n = 3 each group).

  • 1/16

    Green CMFDA purchased from MedChemExpress. Usage Cited in: ACS Infect Dis. 2025 Jun 13;11(6):1563-1576. [Abstract]

    Customer Validation
     Tre-DNP increases phagolysosome fusion. Mabs was either labeled with Tre-DNP or left unlabeled and stained with 20 μM Green CMFDA (1 h) (green). Bacteria were then incubated in buffered saline or opsonized with anti-DNP antibody. The bacteria (2 × 106 CFU/mL) were cocultured with J774 murine macrophages (4 × 105 cells/mL) for 1 h, and extracellular bacteria were eliminated with 100 μg/mL gentamicin. At each time point, cells were stained with 0.5 μM LysoTracker Deep Red (red) and imaged by fluorescence microscopy. Shown are two representative merged images of macrophages 4 h post infection. Yellow depicts overlap between Green CMFDA and LysoTracker Deep Red (B, scale bar = 100 μM)

  • 1/16

    Green CMFDA purchased from MedChemExpress. Usage Cited in: ACS Infect Dis. 2025 Jun 13;11(6):1563-1576. [Abstract]

    Customer Validation
     CMFDA stains mycobacterial pathogens without affecting Tre-DNP labeling. Mabs was labeled with Tre-DNP (250 μM) (or left unlabeled), then washed and stained with Green CMFDA (10 μM; 1 h) (or left unstained). Bacteria were washed, treated with mouse anti-DNP antibody (or left untreated), washed, and stained with PE-conjugated rabbit antimouse IgG antibody. Cells were fixed, washed, and analyzed by flow cytometry. Percentage of the population positive for fluorescein (CMFDA+), representing CMFDA-stained cells.

  • Green CMFDA