JFD00950
JFD00950 is a human FEN1 inhibitor with an IC50 of 5.5 μM. JFD00950 binds directly to FEN1, alters the secondary structure of this protein, and competitively inhibits its flap cleavage activity by interfering with substrate binding. JFD00950 selectively reduces the viability of cancer cells. JFD00950 can be used in the research of colon cancer and breast cancer.
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- CAS. Nr.: 882278-66-2
- Formel: C16H6Cl4O3
- Molecular Weight:388.03
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Speicherung:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biologische Aktivität
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Chemical Information
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Help & FAQs
Biologische Aktivität
JFD00950 (1.25-50 μM; 20 min) potently inhibits purified human FEN1 flap cleavage activity with an IC50 of 5.5 μM[1].
JFD00950 directly binds to purified human FEN1 protein, as shown by significant quenching of the protein's intrinsic fluorescence at 5 μM[1].
JFD00950 (5 μM; 20 min) acts as a competitive inhibitor of purified human FEN1, increasing the apparent Km for the flap DNA substrate while leaving Vmax unchanged at 5 μM[1].
JFD00950 (3.125-50 μM; 48 h) exhibits selective anti-proliferative activity against cancer cell lines, with the highest potency against DLD-1 colon cancer cells (IC50 = 16.17 μM), and minimal activity against normal HEK-293 cells[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:DLD-1, HT-29, HCT-116, MCF-7, MDAMB-231, HEK-293
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Concentration:3.125, 6.25, 12.5, 25, 50 μM
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Incubation Time:48 h
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Result:Inhibited cell growth by 66.60% in DLD-1 cells at 25 μM.
Inhibited cell growth by 50.26% in HT-29 cells at 25 μM.
Inhibited cell growth by 48.8% in HCT-116 cells at 25 μM.
Inhibited cell growth by 46.84% in MCF-7 cells at 25 μM.
Inhibited cell growth by 23.1% in MDAMB-231 cells at 25 μM.
Inhibited cell growth by 20.41% in HEK-293 cells at 25 μM.
Exhibited an IC50 of 16.17 μM for DLD-1 cells.
Chemical Information
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CAS. Nr. 882278-66-2
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Molecular Weight 388.03
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Formel C16H6Cl4O3
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SMILES
O=C1C(Cl)=C(C(C2=C1C=CC=C2)=O)OC3=CC(Cl)=C(C=C3Cl)Cl
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Versand
Room temperature in continental US; may vary elsewhere.
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Speicherung
Please store the product under the recommended conditions in the Certificate of Analysis.
Reinheit & Dokumentation
Verweise
Calculators
Konzentration (Stammlösung) × Volumen (Stammlösung) = Konzentration (Ziellösung) × Volumen (Ziellösung)
JFD00950 Related Classifications
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