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Art. -Nr.: HY-112461

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NF449

7 Cited Publications

Kundenbewertung

Based on 7 publication(s) in Google Scholar

NF449 is a highly potent P2X₁ receptor antagonist, with IC₅₀s of 0.28, 0.69, and 120 nM for rP2X₁, rP2X₁₊₅, P2X₂₊₃, respectively. NF449 is a G_sα-selective G Protein antagonist. NF449 suppresses the rate of GTP[γS] binding to G_sα-s, inhibits the stimulation of adenylyl cyclase activity, and blocks the coupling of β-adrenergic receptors to G_s.

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CAS. Nr.: 389142-38-5
Formel: C₄₁H₃₂N₆O₂₉S₈
Molecular Weight:1329.24

Speicherung:

Please store the product under the recommended conditions in the Certificate of Analysis.

Data Sheet (279 KB)

NF449 Chemical Structure

CAS. Nr.: 389142-38-5

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biologische Aktivität
- Beschreibung
- IC₅₀ & Target
- In Vitro
- In Vivo
Chemical Information
Publications (7)
Reinheit & Dokumentation
Verweise
Calculators
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

IC₅₀ & Target

p2x1 Receptor

Alle P2X Receptor Isoform-spezifische Produkte anzeigen :

In Vitro

NF449 suppressed the rate of GTP[γS] binding to rG_sα-s while barely affecting binding to rG_iα-1 (IC₅₀=140 nM), inhibits stimulation of adenylyl cyclase activity in S49 cyc membranes (deficient in endogenous Gsα) by exogenously added Gsα-s, and blocks the coupling of β-adrenergic receptors to G_s (EC₅₀=7.9 μM)^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

At a dose of 10 mg/kg, NF449 inhibits the ex vivo aggregation triggered by 5 g/ml collagen in WT mouse platelets without affecting that induced by 5 μM ADP. At a higher dose (50 mg/kg), NF449 inhibits ex vivo platelet aggregation in response to not only 10 g/ml collagen but also 5 M ADP, indicating nonselective inhibition of the P2Y1 and/or P2Y12 receptor^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS. Nr. 389142-38-5
Molecular Weight 1329.24
Formel C₄₁H₃₂N₆O₂₉S₈
SMILES

O=C(NC1=CC(C(NC2=CC=C(S(=O)(O)=O)C=C2S(=O)(O)=O)=O)=CC(C(NC3=CC=C(S(=O)(O)=O)C=C3S(=O)(O)=O)=O)=C1)NC4=CC(C(NC5=CC=C(S(=O)(O)=O)C=C5S(=O)(O)=O)=O)=CC(C(NC6=CC=C(S(=O)(O)=O)C=C6S(=O)(O)=O)=O)=C4
Versand

Room temperature in continental US; may vary elsewhere.
Speicherung

Please store the product under the recommended conditions in the Certificate of Analysis.

Publications (7)

All Bio/Physico-chemical Assay

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Journal Impact Factor

Cell Host Microbe

Immunometabolic reprogramming of macrophages by gut microbiota-derived cadaverine controls colon inflammation. [Abstract]

2025 Sep 30:S1931-3128(25)00375-0. PMID: 41033313
Proc Natl Acad Sci U S A

The pyruvate-GPR31 axis promotes transepithelial dendrite formation in human intestinal dendritic cells. [Abstract]

2024 Oct 29;121(44):e2318767121. PMID: 39432783

NF449 purchased from MedChemExpress. Usage Cited in: Proc Natl Acad Sci U S A. 2024 Oct 29;121(44):e2318767121. [Abstract]

NF449 octasodium (10 μM; 30 min), a Gsα-subunit-selective G-protein antagonist，canceled PA-induced dendrite protrusion of iPSC-derived cDC1s.

NF449 purchased from MedChemExpress. Usage Cited in: Proc Natl Acad Sci U S A. 2024 Oct 29;121(44):e2318767121. [Abstract]

NF449 octasodium (10 μM; 30 min), a Gsα antagonist, abolished the significant increase in the percentage of OVA⁺ cells displaying antigen uptake among GPR31-expressing iPSC-derived cDC1s during PA stimulation.
EMBO J

GNAS/PKA signaling promotes aberrant osteochondral differentiation of Gli1+ tendon sheath progenitors. [Abstract]

2025 Sep 1. PMID: 40890486
J Agric Food Chem

Preventive Effect of Caffeic Acid Phenethyl Ester, an Active Component of Propolis, against TNF-α-Induced Endothelial Dysfunction through the β2-Adrenoceptor-Mediated eNOS/NO Signal Pathway. [Abstract]

2026 Apr 22;74(15):12132-12144. PMID: 41957988
Vet Parasitol

Giardia duodenalis cysts induce bovine neutrophil extracellular trap formation. [Abstract]

2026 May:344:110769. PMID: 41967379
Vet Parasitol

Giardia duodenalis trophozoites triggered bovine neutrophil extracellular traps formation dependent on P2X1 receptor and PAD4 in vitro. [Abstract]

2022 Dec:312:109841. PMID: 36427458

NF449 purchased from MedChemExpress. Usage Cited in: Vet Parasitol. 2022 Dec:312:109841. [Abstract]

The purinergic P2X1 receptor's inhibitor NF449 octasodium (10 mM; 30 min) sharply declined Giardia duodenalis trophozoites-triggered NET formation.
bioRxiv

2026 Jun 3.

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (279 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. Rettinger J, et al. Profiling at recombinant homomeric and heteromeric rat P2X receptors identifies the suramin analogue NF449 as a highly potent P2X1 receptor antagonist. Neuropharmacology. 2005;48(3):461-468. [Content Brief]

[2]. Hohenegger M, et al. Gsalpha-selective G protein antagonists. Proc Natl Acad Sci U S A. 1998;95(1):346-351. [Content Brief]

[3]. Hechler B, et al. Inhibition of platelet functions and thrombosis through selective or nonselective inhibition of the platelet P2 receptors with increasing doses of NF449 [4,4',4'',4'''-(carbonylbis(imino-5,1,3-benzenetriylbis-(carbonylimino)))tetrakis-benzene-1,3-disulfonic acid octasodium salt]. J Pharmacol Exp Ther. 2005;314(1):232-243. [Content Brief]

Calculators

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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MOQ

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100 mg

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Biologische Aktivität
- Beschreibung
- IC₅₀ & Target
- In Vitro
- In Vivo
Chemical Information
Publications (7)
Reinheit & Dokumentation
Verweise

1/16
1/16

NF449 purchased from MedChemExpress. Usage Cited in: Proc Natl Acad Sci U S A. 2024 Oct 29;121(44):e2318767121. [Abstract]

Customer Validation
NF449 octasodium (10 μM; 30 min), a Gsα-subunit-selective G-protein antagonist，canceled PA-induced dendrite protrusion of iPSC-derived cDC1s.
1/16

NF449 purchased from MedChemExpress. Usage Cited in: Proc Natl Acad Sci U S A. 2024 Oct 29;121(44):e2318767121. [Abstract]

Customer Validation
NF449 octasodium (10 μM; 30 min), a Gsα antagonist, abolished the significant increase in the percentage of OVA⁺ cells displaying antigen uptake among GPR31-expressing iPSC-derived cDC1s during PA stimulation.
1/16

NF449 purchased from MedChemExpress. Usage Cited in: Vet Parasitol. 2022 Dec:312:109841. [Abstract]

Customer Validation
The purinergic P2X1 receptor's inhibitor NF449 octasodium (10 mM; 30 min) sharply declined Giardia duodenalis trophozoites-triggered NET formation.

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NF449

Publications Citing Use of MedChemExpress (MCE) NF449

Alle P2X Receptor Isoform-spezifische Produkte anzeigen