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Cat. No.: HY-D1931

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ATTO 590

ATTO 590 is a rhodamine-class fluorescent dye that can be used for labeling DNA, RNA, or proteins. ATTO 590 features strong absorption, high fluorescence quantum yield, and excellent thermal and photostability. ATTO 590 can be used in various technical detections such as single-molecule detection, flow cytometry (FACS), and fluorescence in situ hybridization (FISH) (λabs/λem = 593-594/622-624 nM).

For research use only. We do not sell to patients.

CAS No.: 639065-13-7
Formula: C₃₇H₃₉ClN₂O₉
Molecular Weight:691.17

Storage:

Please store the product under the recommended conditions in the Certificate of Analysis.

Data Sheet (271 KB)

ATTO 590 Chemical Structure

CAS No.: 639065-13-7

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Use of MCE Products

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- In Vitro
Chemical Information
Purity & Documentation
References
Calculators
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Biological Activity

Description

In Vitro

ATTO 590 is used as a single-molecule sample or label on the surface of nanobeads, and attached to the microtubule network of fixed PtK2 cells via immunolabeling, for imaging validation of polarization-modulated super-resolution microscopy (SPoD/ExPAN)^[2].
ATTO 590-siRNA conjugates can be used for FRET live-cell imaging in rat brain endothelial cells (RBE4) to track the intracellular uptake and degradation of siRNA^[1].
ATTO 590 complexed with oligonucleotides can be used to label open chromatin in fixed mouse hippocampal CA1 pyramidal neuron tissue sections (ATAC-see technique)^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Related Protocols :

Chemical Information

CAS No. 639065-13-7
Molecular Weight 691.17
Formula C₃₇H₃₉ClN₂O₉
SMILES

CC1=CC(C)(C)N(CC)C2=C1C=C(C(C3=CC=CC=C3C(O)=O)=C(C=C(C(C)=CC(C)(C)N4CC)C4=C5)C5=[O+]6)C6=C2.O=Cl(=O)([O-])=O.CC(O)=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (271 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Hirsch M, et al. Live cell imaging of duplex siRNA intracellular trafficking. Nucleic acids research. 2015 May 19;43(9):4650-60.

[2]. Hafi N, et al. Fluorescence nanoscopy by polarization modulation and polarization angle narrowing. Nature methods. 2014 May;11(5):579-84.

[3]. Meyer SA, et al. Super-resolution imaging of ciliary microdomains in isolated olfactory sensory neurons using a custom two-color stimulated emission depletion microscope. Journal of biomedical optics. 2016 Jun 01;21(6):66017.

[4]. Sheu SH, et al. A serotonergic axon-cilium synapse drives nuclear signaling to alter chromatin accessibility. Cell. 2022 Sep 01;185(18):3390-3407.e18.

[5]. Kumar SR, et al. TLR9-independent CD8+ T cell responses in hepatic AAV gene transfer through IL-1R1-MyD88 signaling. Molecular Therapy. 2024 Feb 7;32(2):325-39. [Content Brief]

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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100 mg

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