FLAG peptide
Based on 26 publication(s) in Google Scholar
FLAG peptide is a multifunctional fusion tag for the purification of recombinant proteins. FLAG peptide maintances the natural folding of its fusing proteins. FLAG peptide can be removed by enterokinase, and eluted under non-denaturing conditions.
For research use only. We do not sell to patients.
- Purity: 99.30%
- CAS No.: 98849-88-8
- Formula: C41H60N10O20
- Molecular Weight:1012.97
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Storage:
Sealed storage, away from moisture and light, under nitrogen.
Powder -80°C, 2 years , -20°C, 1 year* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) FLAG peptide
More- Cell Res. 2023 Dec;33(12):904-922. [Abstract]
- Nat Commun. 2024 Aug 12;15(1):6915. [Abstract]
- Mol Cell. 2025 Apr 4:S1097-2765(25)00260-6. [Abstract]
- Adv Sci (Weinh). 2025 Aug 13:e07777. [Abstract]
- Cell Death Differ. 2023 Feb;30(2):527-543. [Abstract]
- J Integr Plant Biol. 2025 Sep;67(9):2510-2524. [Abstract]
- Sci China Life Sci. 2020 Sep;63(9):1394-1405. [Abstract]
- Oncogene. 2019 Jan;38(5):747-764. [Abstract]
- Free Radic Biol Med. 2022 May 20;185:67-75. [Abstract]
- Cell Rep. 2026 Jan 22;45(2):116900. [Abstract]
- Cell Rep. 2024 Feb 20;43(3):113827. [Abstract]
- Mol Cancer Res. 2025 Nov 3. [Abstract]
- J Lipid Res. 2025 Jan;66(1):100725. [Abstract]
- iScience. 2026 Feb 21;29(3):115119. [Abstract]
- ACS Chem Neurosci. 2024 Oct 2;15(19):3576-3586. [Abstract]
- iScience. 2023 Oct 13;26(11):108207. [Abstract]
- Chemistry. 2023 Jan 9;29(2):e202202083. [Abstract]
- Int J Biochem Cell Biol. 2022 Apr:145:106188. [Abstract]
- STAR Protoc. 2025 Jul 18;6(3):103963. [Abstract]
- bioRxiv. 2025 Dec 21:2025.12.19.695557. [Abstract]
- bioRxiv. 2025 January 21.
- University of California, Davis. 2024.
- bioRxiv. 2024 August 28.
- bioRxiv. 2024 Sep 21:2024.09.17.613574. [Abstract]
- bioRxiv. 2024 Sep 24:2024.09.21.614248. [Abstract]
- bioRxiv. 2023 Aug 19.
Biological Activity
Flag peptide is a highly acidic octapeptide that has a calcium-dependent epitope for monoclonal antibodies, allows elution under non-denaturing conditions, and can be fused to the N-terminus of the target protein. Flag peptide can be captured by the immunoaffinity column in the presence of Ca2+ and eluted by EDTA at low concentrations and neutral pH (thus close to physiological conditions)[1][2].
Reprogramming factors in mammalian cells are purified by FLAG peptide[3]
(1) Transfected cells: HEK293 cells were transfected with FLAG-tagged reprogramming factors, and washed with PBS twice 24 h after transfection.
(2) Collect cells: With NP40 cell lysis buffer (50 mM Tris-HCl, pH 7.4, 250 mM NaCl, 5 mM EDTA, 1% NP-40, and protease inhibitors). Centrifuge the cell lysate at 14,500 rpm for 15 min at 4 °C to collect the soluble fraction.
(3) Add Anti-Flag Affinity Gel(HY-K0217) to the supernatant and incubate in a cold room with rotation for 16 h.
(4) Wash twice with PBS and add 0.1 mg/ml FLAG peptide to elute and retain the FLAG-tagged protein.
(5) The eluted protein is dialysed with a dialysis tubing in dialysis buffer (PBS).
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 98849-88-8
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Appearance Solid
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Molecular Weight 1012.97
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Formula C41H60N10O20
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Color White to off-white
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Sequence
Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys
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Sequence Shortening
DYKDDDDK
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Sealed storage, away from moisture and light, under nitrogen
Powder -80°C 2 years -20°C 1 year * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen)
Publications (26)
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Journal Impact Factor
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Most Recent
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Cell Res
Mannose antagonizes GSDME-mediated pyroptosis through AMPK activated by metabolite GlcNAc-6P. [Abstract]2023 Dec;33(12):904-922. PMID: 37460805 -
Nat Commun
Hypoxia-induced downregulation of PGK1 crotonylation promotes tumorigenesis by coordinating glycolysis and the TCA cycle. [Abstract]2024 Aug 12;15(1):6915. PMID: 39134530 -
Mol Cell
Phosphorylation-dependent charge blocks regulate the relaxation of nuclear speckle networks. [Abstract]2025 Apr 4:S1097-2765(25)00260-6. PMID: 40233760 -
Adv Sci (Weinh)
2025 Aug 13:e07777. PMID: 40799131 -
Cell Death Differ
Targeting USP10 induces degradation of oncogenic ANLN in esophageal squamous cell carcinoma. [Abstract]2023 Feb;30(2):527-543. PMID: 36526897 -
J Integr Plant Biol
A plant viral effector disrupts ALD1-OSB1 immunity module to suppress chloroplast defenses. [Abstract]2025 Sep;67(9):2510-2524. PMID: 40607609 -
Sci China Life Sci
Arginine methylation of ribose-5-phosphate isomerase A senses glucose to promote human colorectal cancer cell survival. [Abstract]2020 Sep;63(9):1394-1405. PMID: 32157557 -
Oncogene
EGF-induced nuclear localization of SHCBP1 activates β-catenin signaling and promotes cancer progression. [Abstract]2019 Jan;38(5):747-764. PMID: 30177836 -
Free Radic Biol Med
Suppression of cideb under endoplasmic reticulum stress exacerbated hepatic inflammation by inducing hepatic steatosis and oxidative stress. [Abstract]2022 May 20;185:67-75. PMID: 35489563 -
Cell Rep
2026 Jan 22;45(2):116900. PMID: 41575859 -
Cell Rep
2024 Feb 20;43(3):113827. PMID: 38381607 -
Mol Cancer Res
Synergistic DBNDD1-GDF15 signaling activates the NF-κB pathway to promote colorectal cancer progression. [Abstract]2025 Nov 3. PMID: 41182793 -
J Lipid Res
A novel mutation, Ile344Asn, in microsomal triglyceride transfer protein abolishes binding to protein disulfide isomerase. [Abstract]2025 Jan;66(1):100725. PMID: 39672332 -
iScience
Dephosphorylation at Ser548 regulates hemocyanin-derived antibacterial peptides and immune defense in Penaeus vannamei. [Abstract]2026 Feb 21;29(3):115119. PMID: 41852743 -
ACS Chem Neurosci
Functional Characterization of an Arylsulfonamide-Based Small-Molecule Inhibitor of the NLRP3 Inflammasome. [Abstract]2024 Oct 2;15(19):3576-3586. PMID: 39297418 -
iScience
Exploring the role of SWI/SNF complex subunit BAF60c in lipid metabolism and inflammation in fish. [Abstract]2023 Oct 13;26(11):108207. PMID: 37942006 -
Chemistry
Rational Design and Synthesis of D-galactosyl Lysophospholipids as Selective Substrates and non-ATP-competitive Inhibitors of Phosphatidylinositol Phosphate Kinases. [Abstract]2023 Jan 9;29(2):e202202083. PMID: 36424188 -
Int J Biochem Cell Biol
AKT serine/threonine kinase 2-mediated phosphorylation of fascin threonine 403 regulates esophageal cancer progression. [Abstract]2022 Apr:145:106188. PMID: 35219877 -
STAR Protoc
Protocol for reconstituting enzymatic activities for ultra-large histone methyltransferases NSD1 and SETD2 using a baculovirus expression system. [Abstract]2025 Jul 18;6(3):103963. PMID: 40684435 -
bioRxiv
2025 Dec 21:2025.12.19.695557. PMID: 41473327 -
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bioRxiv
2024 Sep 21:2024.09.17.613574. PMID: 39345449 -
bioRxiv
Toxicity of extracellular cGAMP and its analogs to T cells is due to SLC7A1-mediated import. [Abstract]2024 Sep 24:2024.09.21.614248. PMID: 39386698 -
Solvent & Solubility
H2O : 100 mg/mL (98.72 mM; Need ultrasonic)
DMSO : 20 mg/mL (19.74 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
For the following dissolution methods, please prepare the working solution directly:
It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: PBS
Solubility: 100 mg/mL (98.72 mM); Clear solution; Need ultrasonic
Purity & Documentation
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Data Sheet (286 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
[1]. Einhauer A, et al. The FLAG peptide, a versatile fusion tag for the purification of recombinant proteins. J Biochem Biophys Methods. 2001 Oct 30;49(1-3):455-65. [Content Brief]
[2]. Schuster M, et al. Protein expression in yeast; comparison of two expression strategies regarding proteinmaturation. J Biotechnol. 2000 Dec 28;84(3):237-48. [Content Brief]
[3]. Min-Joon Han, et al. Purification of functional reprogramming factors in mammalian cell using FLAG –Tag. Biochem Biophys Res Commun. 2017 Oct 14;492(2):154-160. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO / H2O | 1 mM | 0.9872 mL | 4.9360 mL | 9.8720 mL | 24.6799 mL |
| 5 mM | 0.1974 mL | 0.9872 mL | 1.9744 mL | 4.9360 mL | |
| 10 mM | 0.0987 mL | 0.4936 mL | 0.9872 mL | 2.4680 mL | |
| 15 mM | 0.0658 mL | 0.3291 mL | 0.6581 mL | 1.6453 mL | |
| H2O | 20 mM | 0.0494 mL | 0.2468 mL | 0.4936 mL | 1.2340 mL |
| 25 mM | 0.0395 mL | 0.1974 mL | 0.3949 mL | 0.9872 mL | |
| 30 mM | 0.0329 mL | 0.1645 mL | 0.3291 mL | 0.8227 mL | |
| 40 mM | 0.0247 mL | 0.1234 mL | 0.2468 mL | 0.6170 mL | |
| 50 mM | 0.0197 mL | 0.0987 mL | 0.1974 mL | 0.4936 mL | |
| 60 mM | 0.0165 mL | 0.0823 mL | 0.1645 mL | 0.4113 mL | |
| 80 mM | 0.0123 mL | 0.0617 mL | 0.1234 mL | 0.3085 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
FLAG peptide Related Classifications
HY-P0223 Related Classifications
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