Pyronin Y
Based on 4 publication(s) in Google Scholar
Pyronin Y (Pyronine G) is a cationic dye that intercalates RNA and has been used to target cell structures including RNA, DNA and organelles. Pyronin Y forms fluorescent complexes with double-stranded nucleic acids (especially RNA) enabling semi-quantitative analysis of cellular RNA. Pyronin Y can be used to identify specific RNA subspecies of ribonuclear proteins complexes in live cells.
For research use only. We do not sell to patients.
- Purity: 83.71%
- CAS No.: 92-32-0
- Formula: C17H19ClN2O
- Molecular Weight:302.80
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Storage:
-20°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Protocol
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) Pyronin Y
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Biological Activity
Pyronin Y forms fluorescent complexes with double-stranded nucleic acids, especially RNA, enabling semi-quantitative analysis of cellular RNA in flow cytometry, to estimate the RNA content per cell in formalin fixed EL4 leukosis tumor cells, enzyme dispersed R3327-G rat prostatic adenocarcinoma cells, mouse spleen cells stimulated with concanavalin A, and human peripheral blood lymphocytes stimulated with phytohemagglutinin[1].
Pyronin Y is almost entirely localized in the mitochondria of cultured cells in living cells. At concentrations of 1.7 to 3.3 μM, it is non-toxic but inhibits cell growth, causing cell arrest[3].
Pyronin Y has long been used, in combination with other dyes such as Methyl Green, as a differential stain for nucleic acids in paraffin tissue sections. In sections stained with Methyl Green-pyronin Y, red blood cells, elastic fibre of blood vessels, zymogen granules of pancreatic acinar cells, surface membrane of heptocytes and kidney tubular cells showed strikingly strong green and/or red fluorescence, while the nuclei of cells appeared non-fluorescent[4].
Pyronin Y binds to double stranded RNA (dsRNA) including messenger RNA (mRNA), transfer RNA (tRNA) and ribosomal RNA (rRNA)[5].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 92-32-0
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Appearance Solid
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Molecular Weight 302.80
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Formula C17H19ClN2O
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Color Light brown to black
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SMILES
CN(C1=CC2=[O+]C3=C(C=CC(N(C)C)=C3)C=C2C=C1)C.[Cl-]
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Synonyms
Pyronine G; C.I. 45005
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
-20°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
Publications (4)
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Journal Impact Factor
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Most Recent
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Nat Commun
2021 Nov 30;12(1):6971. PMID: 34848703 -
Adv Healthc Mater
Inducing In Situ Functional Maturation of Transplanted Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes: Establishing Strategies for Treating Myocardial Injury. [Abstract]2025 Oct 21:e03799. PMID: 41121551 -
EMBO J
Maternal mRNA deadenylation and allocation via Rbm14 condensates facilitate vertebrate blastula development. [Abstract]2023 Feb 1;42(3):e111364. PMID: 36477743 -
Transl Oncol
Matrix stiffness induces an invasive-dormant subpopulation via cGAS-STING axis in oral cancer. [Abstract]2023 Jul:33:101681. PMID: 37137218
Solvent & Solubility
DMSO : 25 mg/mL (82.56 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : 4 mg/mL (13.21 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Protocol
The cells are resuspended and stained for 30 min in 1.0 mL of 0.01% pyronin Y in sodiumacetate buffer (1.0 M, pH 4.7). For this purpose 1.0 g of pyronin Y is dissolved in 100 mL distilled water and this solution is purified by chloroform extraction (three fractions of 100 mL). The purified pyronin Y solution is diluted with 1.0 M sodiumacetate buffer pH 4.7 to the appropriate concentration and filtered through paper filters before use. Stained cell samples are studied by fluorescence microscopy (excitation filters SP 560 and LP 515, chromatic beam splitter at 580 nm, barrier filter LP 580) or by flow cytometry[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Purity & Documentation
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Data Sheet (275 KB)
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SDS (644 KB)
- English - EN (644 KB)
- Français - FR (644 KB)
- Deutsch - DE (644 KB)
- Norwegian - NO (644 KB)
- Español - ES (644 KB)
- Swedish - SV (644 KB)
- Italian - IT (644 KB)
- Korean - KR (644 KB)
- Portuguese - PT (644 KB)
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Handling Instructions (2659 KB)
References
[1]. Pollack A, et al. Flow cytometric analysis of RNA content in different cell populations using pyronin Yand methyl green. Cytometry. 1982 Jul;3(1):28-35. [Content Brief]
[2]. Tanke HJ, et al. Flow cytometry of human reticulocytes based on RNA fluorescence. Cytometry. 1981 Mar;1(5):313-20. [Content Brief]
[3]. Darzynkiewicz Z, et al. Cytostatic and cytotoxic properties of pyronin Y: relation to mitochondrial localization of the dye and its interaction with RNA. Cancer Res. 1986 Nov;46(11):5760-6. [Content Brief]
[4]. Li B, et al. Pyronin Y as a fluorescent stain for paraffin sections. Histochem J. 2002 Jun-Jul;34(6-7):299-303. [Content Brief]
[5]. Laura M Andrews, et al. Spectral phasor analysis of Pyronin Y labeled RNA microenvironments in living cells. Biomed Opt Express. 2013 Jan 1;4(1):171-7. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O / DMSO | 1 mM | 3.3025 mL | 16.5126 mL | 33.0251 mL | 82.5628 mL |
| 5 mM | 0.6605 mL | 3.3025 mL | 6.6050 mL | 16.5126 mL | |
| 10 mM | 0.3303 mL | 1.6513 mL | 3.3025 mL | 8.2563 mL | |
| DMSO | 15 mM | 0.2202 mL | 1.1008 mL | 2.2017 mL | 5.5042 mL |
| 20 mM | 0.1651 mL | 0.8256 mL | 1.6513 mL | 4.1281 mL | |
| 25 mM | 0.1321 mL | 0.6605 mL | 1.3210 mL | 3.3025 mL | |
| 30 mM | 0.1101 mL | 0.5504 mL | 1.1008 mL | 2.7521 mL | |
| 40 mM | 0.0826 mL | 0.4128 mL | 0.8256 mL | 2.0641 mL | |
| 50 mM | 0.0661 mL | 0.3303 mL | 0.6605 mL | 1.6513 mL | |
| 60 mM | 0.0550 mL | 0.2752 mL | 0.5504 mL | 1.3760 mL | |
| 80 mM | 0.0413 mL | 0.2064 mL | 0.4128 mL | 1.0320 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Pyronin Y Related Classifications
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