UBD1031
UBD1031 (SGC-UBD1031) is a selective USP16/HDAC6-UBD antagonist with a human USP16-UBD IC50 of 10.6 μM, KD value of 48 nM, and a human HDAC6-UBD KD of 16 nM. UBD1031 disrupts ISG15 C-terminus interactions with USP16-UBD and HDAC6-UBD, and exhibits cooperative inhibition of full-length USP16 deubiquitinase activity via USP16-UBD binding. UBD1031 can be used in research on cancer, autoimmune diseases, and Down syndrome.
For research use only. We do not sell to patients.
- Formula: C21H18ClF2N3O4
- Molecular Weight:449.84
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
In Vitro
UBD1031 potently binds purified USP16-UBD (residues 25-185) with an SPR-determined KD of 48 nM[1].
UBD1031 potently binds purified HDAC6-UBD (residues 1109-1215) with an SPR-determined KD of 16 nM[1].
UBD1031 (0.01 nM-10 μM) inhibits the deubiquitinase activity of purified full-length USP16 (USP161-823) with an IC50 of 10.6 μM, reaching up to 60% inhibition at maximal tested concentrations[1].
UBD1031 is at least 100-fold selective for USP16-UBD and HDAC6-UBD over seven other tested purified UBD-containing proteins (USP3, USP5, USP13, USP33, USP49, USP51, BRAP)[1].
UBD1031 (0.1-10 μM; 4 h) disrupts the interaction between USP16 and ISG15 in HEK293T cells with an IC50 of 1.7 μM[1].
UBD1031 (0.1-10 μM; 4 h) disrupts the interaction between HDAC6 and ISG15 in HEK293T cells with an IC50 of 1.5 μM[1].
UBD1031 (30 μM; 5 day) shows no detectable toxicity to HEK293T or HCT116 cells[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:HEK293T and HCT116 cells
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Concentration:30 μM
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Incubation Time:5 day
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Result:No reduction in cell confluency was observed relative to DMSO-treated controls in either cell line.
Chemical Information
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Molecular Weight 449.84
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Formula C21H18ClF2N3O4
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SMILES
OC(CCC1=NC2=C(C(N1CC(N[C@@H](C)C3=C(C=CC=C3F)F)=O)=O)C=CC=C2Cl)=O
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Synonyms
SGC-UBD1031
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Solvent & Solubility
In Vitro:
DMSO : 100 mg/mL (222.30 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 5 mg/mL (11.12 mM); Clear solution
This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
References
Complete Stock Solution Preparation Table
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.2230 mL | 11.1151 mL | 22.2301 mL | 55.5753 mL |
| 5 mM | 0.4446 mL | 2.2230 mL | 4.4460 mL | 11.1151 mL | |
| 10 mM | 0.2223 mL | 1.1115 mL | 2.2230 mL | 5.5575 mL | |
| 15 mM | 0.1482 mL | 0.7410 mL | 1.4820 mL | 3.7050 mL | |
| 20 mM | 0.1112 mL | 0.5558 mL | 1.1115 mL | 2.7788 mL | |
| 25 mM | 0.0889 mL | 0.4446 mL | 0.8892 mL | 2.2230 mL | |
| 30 mM | 0.0741 mL | 0.3705 mL | 0.7410 mL | 1.8525 mL | |
| 40 mM | 0.0556 mL | 0.2779 mL | 0.5558 mL | 1.3894 mL | |
| 50 mM | 0.0445 mL | 0.2223 mL | 0.4446 mL | 1.1115 mL | |
| 60 mM | 0.0371 mL | 0.1853 mL | 0.3705 mL | 0.9263 mL | |
| 80 mM | 0.0278 mL | 0.1389 mL | 0.2779 mL | 0.6947 mL | |
| 100 mM | 0.0222 mL | 0.1112 mL | 0.2223 mL | 0.5558 mL |
UBD1031 Related Classifications
HY-172193 Related Classifications
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