Aurora kinase inhibitor-8
Based on 1 Customer Validation
Aurora kinase inhibitor-8 is a highly selective inhibitor of the Aurora kinases.
For research use only. We do not sell to patients.
- Purity: 98.01%
- CAS No.: 2133001-88-2
- Formula: C30H29N7O3
- Molecular Weight:535.60
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Storage:
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Bioactive Compound Library Max
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Bioactive Compound Library
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Anti-Aging Compound Library
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All Aurora Kinase Isoforms
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Biological Activity
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| HCT-116 | IC50 |
0.01012 μM
Compound: 2; FMF-03-145-1
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Cytotoxicity against human HCT116 cells assessed as reduction in cell viability after 72 hrs by CellTiter-Glo assay
Cytotoxicity against human HCT116 cells assessed as reduction in cell viability after 72 hrs by CellTiter-Glo assay
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[PMID: 28818446] |
| HEK293 | IC50 |
0.253 μM
Compound: 2; FMF-03-145-1
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Cytotoxicity against human HEK293 cells assessed as reduction in cell viability after 72 hrs by CellTiter-Glo assay
Cytotoxicity against human HEK293 cells assessed as reduction in cell viability after 72 hrs by CellTiter-Glo assay
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[PMID: 28818446] |
| HeLa | IC50 |
1.322 x 10-6 μM
Compound: 2; FMF-03-145-1
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Cytotoxicity against human HeLa cells assessed as reduction in cell viability after 72 hrs by CellTiter-Glo assay
Cytotoxicity against human HeLa cells assessed as reduction in cell viability after 72 hrs by CellTiter-Glo assay
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[PMID: 28818446] |
| PC-3 | IC50 |
0.9882 μM
Compound: 2; FMF-03-145-1
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Cytotoxicity against human PC3 cells assessed as reduction in cell viability after 72 hrs by CellTiter-Glo assay
Cytotoxicity against human PC3 cells assessed as reduction in cell viability after 72 hrs by CellTiter-Glo assay
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[PMID: 28818446] |
Chemical Information
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CAS No. 2133001-88-2
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Appearance Solid
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Molecular Weight 535.60
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Formula C30H29N7O3
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Color Off-white to light yellow
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SMILES
O=C(NC1=CC=C(NC2=NC=C(N3C)C(N(C)C4=CC=C(C)C=C4C3=O)=N2)C=C1)C5=CC=C(NC(CC)=O)C=C5
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
Solvent & Solubility
DMSO : 25 mg/mL (46.68 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 1.25 mg/mL (2.33 mM); Clear solution
This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (270 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.8671 mL | 9.3353 mL | 18.6707 mL | 46.6766 mL |
| 5 mM | 0.3734 mL | 1.8671 mL | 3.7341 mL | 9.3353 mL | |
| 10 mM | 0.1867 mL | 0.9335 mL | 1.8671 mL | 4.6677 mL | |
| 15 mM | 0.1245 mL | 0.6224 mL | 1.2447 mL | 3.1118 mL | |
| 20 mM | 0.0934 mL | 0.4668 mL | 0.9335 mL | 2.3338 mL | |
| 25 mM | 0.0747 mL | 0.3734 mL | 0.7468 mL | 1.8671 mL | |
| 30 mM | 0.0622 mL | 0.3112 mL | 0.6224 mL | 1.5559 mL | |
| 40 mM | 0.0467 mL | 0.2334 mL | 0.4668 mL | 1.1669 mL |
Aurora kinase inhibitor-8 Related Classifications
HY-144991 Related Classifications
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