2. Metabolic Enzyme/Protease
  3. Phosphodiesterase (PDE)
  4. AWD 12-281

AWD 12-281 is an orally active and highly selective phosphodiesterase 4 (PDE4) inhibitor (IC50 = 9.7 nM). AWD 12-281 is used in the study of allergic dermatitis, asthma, chronic obstructive pulmonary disease (COPD), and allergic rhinitis.

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AWD 12-281

AWD 12-281 Chemical Structure

CAS No. : 257892-33-4

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
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10 mM * 1 mL in DMSO In-stock
Solid
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AWD 12-281 Related Antibodies

Top Publications Citing Use of Products

View All Phosphodiesterase (PDE) Isoform Specific Products:

View All Isoforms
PDE1 PDE2 PDE3 PDE4 PDE5 PDE6 PDE7 PDE9 PDE10 PDE11 PDE12 PDE8 Autotaxin

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Description

AWD 12-281 is an orally active and highly selective phosphodiesterase 4 (PDE4) inhibitor (IC50 = 9.7 nM). AWD 12-281 is used in the study of allergic dermatitis, asthma, chronic obstructive pulmonary disease (COPD), and allergic rhinitis[1][2].

IC50 & Target[1]

PDE4

9.7 nM (IC50)

Cellular Effect
Cell Line Type Value Description References
U-937 IC50
14 nM
Compound: AWD 12-281
Inhibition of PDE4 isolated from human U937 cells using [3H]-cAMP as substrate after 30 mins by liquid scintillation counting analysis
Inhibition of PDE4 isolated from human U937 cells using [3H]-cAMP as substrate after 30 mins by liquid scintillation counting analysis
10.1039/C0MD00215A
Molecular Weight

458.27

Formula

C22H14Cl2FN3O3
CAS No.
Appearance

Solid

SMILES

O=C(C(C1=CN(CC2=CC=C(C=C2)F)C3=C1C=C(O)C=C3)=O)NC4=C(Cl)C=NC=C4Cl
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

-20°C, protect from light, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

Solvent & Solubility
In Vitro: 

DMSO : 175 mg/mL (381.87 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.1821 mL 10.9106 mL 21.8212 mL
5 mM 0.4364 mL 2.1821 mL 4.3642 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo Dissolution Calculator
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Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.1821 mL 10.9106 mL 21.8212 mL 54.5530 mL
5 mM 0.4364 mL 2.1821 mL 4.3642 mL 10.9106 mL
10 mM 0.2182 mL 1.0911 mL 2.1821 mL 5.4553 mL
15 mM 0.1455 mL 0.7274 mL 1.4547 mL 3.6369 mL
20 mM 0.1091 mL 0.5455 mL 1.0911 mL 2.7276 mL
25 mM 0.0873 mL 0.4364 mL 0.8728 mL 2.1821 mL
30 mM 0.0727 mL 0.3637 mL 0.7274 mL 1.8184 mL
40 mM 0.0546 mL 0.2728 mL 0.5455 mL 1.3638 mL
50 mM 0.0436 mL 0.2182 mL 0.4364 mL 1.0911 mL
60 mM 0.0364 mL 0.1818 mL 0.3637 mL 0.9092 mL
80 mM 0.0273 mL 0.1364 mL 0.2728 mL 0.6819 mL
100 mM 0.0218 mL 0.1091 mL 0.2182 mL 0.5455 mL
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AWD 12-281 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

AWD 12-281257892-33-4GW 842470GW842470GW-842470Phosphodiesterase (PDE)allergic dermatitisasthmachronic obstructive pulmonary diseaseCOPDallergic rhinitisInhibitorinhibitorinhibit

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