Azide-PEG8-Tos
Azide-PEG8-Tos is a small PEG linker featuring an azide and a tosylate. Tosylates are good leaving groups which are easily displaced by nucleophiles while azide is a click chemistry motif which is used to react with terminal alkynes or strained cyclooctynes.
For research use only. We do not sell to patients.
- CAS No.: 857892-34-3
- Formula: C23H39N3O10S
- Molecular Weight:549.63
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
- Protocols
- Purity & Documentation
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Help & FAQs
Biological Activity
Description
Chemical Information
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CAS No. 857892-34-3
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Molecular Weight 549.63
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Formula C23H39N3O10S
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SMILES
O=S(C1=CC=C(C=C1)C)(OCCOCCOCCOCCOCCOCCOCCOCCN=[N+]=[N-])=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocols
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EdU Incorporation Assay (Click Chemistry-Based DNA Synthesis Measurement)
The EdU incorporation assay measures DNA synthesis by adding the thymidine analog 5-ethynyl-2′-deoxyuridine to cells or tissues, where it is incorporated into newly synthesized DNA during S phase. Incorporated EdU is detected by copper-catalyzed azide-alkyne cycloaddition, in which a fluorescent azide covalently reacts with the ethynyl group on EdU, allowing S-phase cells to be detected by fluorescence microscopy, flow cytometry, or high-content imaging. EdU detection does not require DNA denaturation or anti-BrdU antibody access, which preserves sample structure and improves compatibility with immunostaining and multiparameter cytometry compared with BrdU-based detection. EdU can be cytotoxic in a cell-type- and exposure-dependent manner, so pulse duration, concentration, and continuous-labeling designs should be validated for each cell type.
Purity & Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Azide-PEG8-Tos Related Classifications
HY-W190738 Related Classifications
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