BC‐AZIDE
BC-AZIDE (CLIP-AZIDE) is a nitrogen-functionalized protein labeling probe based on the CLIP-tag system. BC-AZIDE can be used for bioorthogonal click chemistry and intracellular orthogonal labeling.
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- No. CAS: 2432950-51-9
- Fòrmula: C18H23N7O4
- Peso molecular:401.42
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Almacenamiento:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Actividad biológica
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Chemical Information
- Protocolo
- Pureza y Documentación
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Help & FAQs
Actividad biológica
Descripciòn
Chemical Information
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No. CAS 2432950-51-9
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Peso molecular 401.42
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Fòrmula C18H23N7O4
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SMILES
[N-]=[N+]=NCCOCCOCC(NCC1=CC=C(C=C1)COC2=NC(N)=CC=N2)=O
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Synonyms
CLIP-AZIDE
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocolo
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EdU Incorporation Assay (Click Chemistry-Based DNA Synthesis Measurement)
The EdU incorporation assay measures DNA synthesis by adding the thymidine analog 5-ethynyl-2′-deoxyuridine to cells or tissues, where it is incorporated into newly synthesized DNA during S phase. Incorporated EdU is detected by copper-catalyzed azide-alkyne cycloaddition, in which a fluorescent azide covalently reacts with the ethynyl group on EdU, allowing S-phase cells to be detected by fluorescence microscopy, flow cytometry, or high-content imaging. EdU detection does not require DNA denaturation or anti-BrdU antibody access, which preserves sample structure and improves compatibility with immunostaining and multiparameter cytometry compared with BrdU-based detection. EdU can be cytotoxic in a cell-type- and exposure-dependent manner, so pulse duration, concentration, and continuous-labeling designs should be validated for each cell type.
Pureza y Documentación
Referencias
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
BC‐AZIDE Related Classifications
HY-D2955 Related Classifications
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