Biotin-(Oaa)3-epoxomicin

Name: Biotin-(Oaa)3-epoxomicin
Brand: MedChemExpress
SKU: HY-P11306
Rating: 4.5 (1 reviews)

Cat. No.: HY-P11306 Purity: 96.46%: Data Sheet
COA

SDS
Handling Instructions
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Biotin-(Oaa)₃-epoxomicin is a biotin-labeled form of Epoxomicin (HY-13821), prepared by conjugating Epoxomicin with biotin via three hydrophilic oxaacetyl amino acid (Oaa) linkers. Biotin-(Oaa)₃-epoxomicin is primarily used in proteomic studies for the capture, identification and target validation of proteasome complexes, to determine the intracellular targets of epoxomicin. Epoxomicin acts as a proteasome inhibitor and NF-κB inhibitor, which effectively blocks inflammatory responses in mouse ear edema assays. It inhibits proteasome activity via covalent binding to catalytic subunits including LMP7, X, MECL1 and Z, with the strongest inhibitory effect on chymotrypsin-like activity, and does not interfere with non-proteasomal proteases such as trypsin and papain.

For research use only. We do not sell to patients.

Custom Peptide Synthesis

Biotin-(Oaa)3-epoxomicin Chemical Structure

CAS No. : 247068-92-4

Size	Stock	Quantity
1 mg	In-stock	Estimated Time of Arrival: December 31
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
25 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	Get quote
100 mg	Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

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Biological Activity
Purity & Documentation
References
Customer Review

Description

In Vitro

Epoxomicin (5-8 μM; 4 hr) covalently binds to the LMP7, MECL1, Z, and X catalytic β subunits of the proteasome in EL4 murine thymoma cells and LCL 721.45/LCL 721.174 human B cell lymphoma cells^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

1078.45

Formula

C₅₄H₉₅N₉O₁₁S

CAS No.

247068-92-4

Appearance

Solid

Color

White to off-white

Sequence

Biotin-{Oaa}-{Oaa}-{Oaa}-Ile-Ile-Thr-{Leu((2R)-2-methyloxiranyl)}

Sequence Shortening

Biotin-{Oaa}-{Oaa}-{Oaa}-IIT-{Leu((2R)-2-methyloxiranyl)}

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Sealed storage, away from moisture

Powder	-80°C	2 years
	-20°C	1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

DMSO : ≥ 100 mg/mL (92.73 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	0.9273 mL	4.6363 mL	9.2726 mL
5 mM	0.1855 mL	0.9273 mL	1.8545 mL
10 mM	0.0927 mL	0.4636 mL	0.9273 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

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In Vivo Dissolution Calculator

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Working solution concentration: mg/mL

Purity & Documentation

Purity: 96.46%

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Data Sheet (270 KB) SDS (252 KB)

COA (243 KB) RP-HPLC (112 KB) MS (215 KB)

Handling Instructions (2659 KB)

References

[1]. Meng L, et al. Epoxomicin, a potent and selective proteasome inhibitor, exhibits in vivo antiinflammatory activity. Proc Natl Acad Sci U S A. 1999;96(18):10403-10408. [Content Brief]

[2]. Hanada M, et al. Epoxomicin, a new antitumor agent of microbial origin. J Antibiot (Tokyo). 1992;45(11):1746-1752. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	0.9273 mL	4.6363 mL	9.2726 mL	23.1814 mL
	5 mM	0.1855 mL	0.9273 mL	1.8545 mL	4.6363 mL
	10 mM	0.0927 mL	0.4636 mL	0.9273 mL	2.3181 mL
	15 mM	0.0618 mL	0.3091 mL	0.6182 mL	1.5454 mL
	20 mM	0.0464 mL	0.2318 mL	0.4636 mL	1.1591 mL
	25 mM	0.0371 mL	0.1855 mL	0.3709 mL	0.9273 mL
	30 mM	0.0309 mL	0.1545 mL	0.3091 mL	0.7727 mL
	40 mM	0.0232 mL	0.1159 mL	0.2318 mL	0.5795 mL
	50 mM	0.0185 mL	0.0927 mL	0.1855 mL	0.4636 mL
	60 mM	0.0155 mL	0.0773 mL	0.1545 mL	0.3864 mL
	80 mM	0.0116 mL	0.0580 mL	0.1159 mL	0.2898 mL