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  3. Biotin-PEG10-azide

Biotin-PEG10-azide  (Synonyms: Biotin-PEG10-N3)

Cat. No.: HY-W190862 Purity: 98.0%
Handling Instructions Technical Support

Biotin-PEG10-azide (Biotin-PEG10-N3) is a PEG derivative composed of biotin, 10 PEG units, and an azide group. Biotin can form a stable non-covalent bond with streptavidin. It contains an azide group and can undergo copper-catalyzed azide-alkyne cycloaddition (CuAAc) with molecules containing alkyne groups. It can also undergo ring strain-driven alkyne-azide cycloaddition (SPAAC) with molecules containing DBCO or BCN groups.

For research use only. We do not sell to patients.

Biotin-PEG10-azide

Biotin-PEG10-azide Chemical Structure

CAS No. : 1628165-01-4

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Based on 1 publication(s) in Google Scholar

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Description

Biotin-PEG10-azide (Biotin-PEG10-N3) is a PEG derivative composed of biotin, 10 PEG units, and an azide group. Biotin can form a stable non-covalent bond with streptavidin. It contains an azide group and can undergo copper-catalyzed azide-alkyne cycloaddition (CuAAc) with molecules containing alkyne groups. It can also undergo ring strain-driven alkyne-azide cycloaddition (SPAAC) with molecules containing DBCO or BCN groups.

Molecular Weight

752.92

Formula

C32H60N6O12S

CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O=C(CCCC[C@@H]1SC[C@]([H])(N2)[C@@]1(NC2=O)[H])NCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCN=[N+]=[N-]

Shipping

Room temperature in continental US; may vary elsewhere.

Storage
Powder -20°C 3 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : ≥ 100 mg/mL (132.82 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.3282 mL 6.6408 mL 13.2816 mL
5 mM 0.2656 mL 1.3282 mL 2.6563 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

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Volume (start)

V1

=
Concentration (final)

C2

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Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

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g

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(per animal)

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Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.3282 mL 6.6408 mL 13.2816 mL 33.2041 mL
5 mM 0.2656 mL 1.3282 mL 2.6563 mL 6.6408 mL
10 mM 0.1328 mL 0.6641 mL 1.3282 mL 3.3204 mL
15 mM 0.0885 mL 0.4427 mL 0.8854 mL 2.2136 mL
20 mM 0.0664 mL 0.3320 mL 0.6641 mL 1.6602 mL
25 mM 0.0531 mL 0.2656 mL 0.5313 mL 1.3282 mL
30 mM 0.0443 mL 0.2214 mL 0.4427 mL 1.1068 mL
40 mM 0.0332 mL 0.1660 mL 0.3320 mL 0.8301 mL
50 mM 0.0266 mL 0.1328 mL 0.2656 mL 0.6641 mL
60 mM 0.0221 mL 0.1107 mL 0.2214 mL 0.5534 mL
80 mM 0.0166 mL 0.0830 mL 0.1660 mL 0.4151 mL
100 mM 0.0133 mL 0.0664 mL 0.1328 mL 0.3320 mL
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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Product Name:
Biotin-PEG10-azide
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HY-W190862
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