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Cat. No.: HY-D3435

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Biotin-X-DHPE

Biotin-X-DHPE is a biotin-labeled phospholipid analog that can be used for semi-automated cell panning in antibody discovery. Biotin-X-DHPE enables stable immobilization of live cells on magnetic beads without damaging the cells or altering their surface protein expression levels, while ensuring that the selected antibodies recognize natural, functional epitopes on the cell surface.

For research use only. We do not sell to patients.

Formula: C₅₉H₁₁₄N₅O₁₁PS
Molecular Weight:1132.60

Storage:

Please store the product under the recommended conditions in the Certificate of Analysis.

Biotin-X-DHPE Chemical Structure

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- In Vitro
Chemical Information
Purity & Documentation
References
Calculators
Help & FAQs
Classifications
Bioz

Biological Activity

Description

In Vitro

Biotin-X-DHPE can integrate into the membrane of living cells with minimal damage, and form a streptavidin-magnetic bead complex via either the direct method (with better efficacy) or the indirect method. Optimal cell labeling efficiency is achieved after incubation at room temperature for 30 minutes in Pluronic F-68 buffer^[1].
Biotin-X-DHPE is incorporated into sucrose-protected lyophilized liposome formulations for oligonucleotide detection in electrochemical DNA biosensors^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

Molecular Weight 1132.60
Formula C₅₉H₁₁₄N₅O₁₁PS
SMILES

CCN(CC)CC.CCCCCCCCCCCCCCCC(OC(COC(CCCCCCCCCCCCCCC)=O)COP(OCCNC(CCCCCNC(CCCC[C@]1([H])SCC2C1NC(N2)=O)=O)=O)(O)=O)=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation

Handling Instructions (2659 KB)

References

[1]. Min B, et al. Semi-Automated Cell Panning for Efficient Isolation of FGFR3-Targeting Antibody. International journal of molecular sciences. 2021 Jun 09;22(12):6240.

[2]. Mahmoudi-Badiki T, et al. Dopamine-loaded liposome and its application in electrochemical DNA biosensor. Journal of biomaterials applications. 2016 Aug;31(2):273-82.

Calculators

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Dilution Calculator

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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100 mg

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