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  3. Fluorescent Dye
  4. Biotin-X-NTA

Biotin-X-NTA can be used for detecting histidine-tagged proteins immobilized on nitrocellulose membranes.

For research use only. We do not sell to patients.

Biotin-X-NTA Chemical Structure

Biotin-X-NTA Chemical Structure

CAS No. : 856661-92-2

Size Price Stock Quantity
5 mg USD 350 In-stock
10 mg USD 560 In-stock
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100 mg   Get quote  

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This product is a controlled substance and not for sale in your territory.

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Based on 1 publication(s) in Google Scholar

Biotin-X-NTA Related Antibodies

Top Publications Citing Use of Products

  • Biological Activity

  • Purity & Documentation

  • References

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Description

Biotin-X-NTA can be used for detecting histidine-tagged proteins immobilized on nitrocellulose membranes[1].

Molecular Weight

715.98

Formula

C26H40K3N5O9S
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O=C(O[K])CN(CC(O[K])=O)[C@H](C(O[K])=O)CCCCNC(CCCCCNC(CCCC[C@H]1[C@]2([H])[C@](NC(N2)=O)([H])CS1)=O)=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

-20°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

H2O : 100 mg/mL (139.67 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.3967 mL 6.9834 mL 13.9669 mL
5 mM 0.2793 mL 1.3967 mL 2.7934 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
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Concentration
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Volume
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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

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Volume (start)

V1

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Concentration (final)

C2

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Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation

Purity: 98.11%

COA (244 KB) HNMR (300 KB) RP-HPLC (153 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O 1 mM 1.3967 mL 6.9834 mL 13.9669 mL 34.9172 mL
5 mM 0.2793 mL 1.3967 mL 2.7934 mL 6.9834 mL
10 mM 0.1397 mL 0.6983 mL 1.3967 mL 3.4917 mL
15 mM 0.0931 mL 0.4656 mL 0.9311 mL 2.3278 mL
20 mM 0.0698 mL 0.3492 mL 0.6983 mL 1.7459 mL
25 mM 0.0559 mL 0.2793 mL 0.5587 mL 1.3967 mL
30 mM 0.0466 mL 0.2328 mL 0.4656 mL 1.1639 mL
40 mM 0.0349 mL 0.1746 mL 0.3492 mL 0.8729 mL
50 mM 0.0279 mL 0.1397 mL 0.2793 mL 0.6983 mL
60 mM 0.0233 mL 0.1164 mL 0.2328 mL 0.5820 mL
80 mM 0.0175 mL 0.0873 mL 0.1746 mL 0.4365 mL
100 mM 0.0140 mL 0.0698 mL 0.1397 mL 0.3492 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Biotin-X-NTA Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Biotin-X-NTA856661-92-2Fluorescent DyeHistidineNitrocellulose membranesInhibitorinhibitorinhibit

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