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  2. HCV
  3. BLT-1

BLT-1  (Synonyms: Block lipid transport-1)

Cat. No.: HY-116767 Purity: 98.48%
COA Handling Instructions

BLT-1, a thiosemicarbazone copper chelator, is a selective scavenger receptor B, type 1 (SR-BI) inhibitor. BLT-1 inhibits the transfer of lipids between high-density lipoproteins (HDL) and cells mediated by SR-BI. BLT-1 is a potent HCV entry inhibitor.

For research use only. We do not sell to patients.

BLT-1 Chemical Structure

BLT-1 Chemical Structure

CAS No. : 321673-30-7

Size Price Stock Quantity
Solid + Solvent
10 mM * 1 mL in DMSO
ready for reconstitution
USD 121 In-stock
Solution
10 mM * 1 mL in DMSO USD 121 In-stock
Solid
5 mg USD 110 In-stock
10 mg USD 200 In-stock
50 mg USD 550 In-stock
100 mg   Get quote  
200 mg   Get quote  

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This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 4 publication(s) in Google Scholar

Top Publications Citing Use of Products

    BLT-1 purchased from MedChemExpress. Usage Cited in: Cancer Discov. 2022 Nov 4;CD-22-0535.  [Abstract]

    Inhibition of the SR-B1 using the selective chemical inhibitor BLT-1 (5 µM) results in a modestly enhance DC661 cytotoxicity.
    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    BLT-1, a thiosemicarbazone copper chelator, is a selective scavenger receptor B, type 1 (SR-BI) inhibitor. BLT-1 inhibits the transfer of lipids between high-density lipoproteins (HDL) and cells mediated by SR-BI. BLT-1 is a potent HCV entry inhibitor[1][2][3][4].

    In Vitro

    BLT-1 has IC50s of 60 and 110 nM for cellular DiI-HDL and [3H]CE-HDL uptake in ldlA[mSR-BI] cells[1].
    BLT-1 has an IC50 of 0.96 μM for the HCV entry in Huh 7.5.1 cells[4].
    BLT-1 (50 μM; 3 hours) does not induce general defects in clathrin-dependent and -independent intracellular membrane trafficking in HeLa, BSC-1 cells[1].
    BLT-1 can inhibit SR-BI-dependent selective uptake of [3H]CE from [3H]CE-HDL by mSR-BI-t1-containing liposomes in cells (IC50=0.057 μM) and liposomes (IC50=0.098 μM) [2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Molecular Weight

    241.40

    Formula

    C12H23N3S

    CAS No.
    Appearance

    Solid

    Color

    White to light yellow

    SMILES

    CCCCCCC1CCC/C1=N/NC(N)=S

    Shipping

    Room temperature in continental US; may vary elsewhere.

    Storage
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 2 years
    -20°C 1 year
    Solvent & Solubility
    In Vitro: 

    DMSO : 41.67 mg/mL (172.62 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 4.1425 mL 20.7125 mL 41.4250 mL
    5 mM 0.8285 mL 4.1425 mL 8.2850 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
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    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

    ×
    Volume (start)

    V1

    =
    Concentration (final)

    C2

    ×
    Volume (final)

    V2

    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.08 mg/mL (8.62 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: 2.08 mg/mL (8.62 mM); Suspended solution; Need ultrasonic

      This protocol yields a suspended solution of 2.08 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
    DMSO +
    +
    %
    Tween-80 +
    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    Purity & Documentation

    Purity: 98.48%

    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 4.1425 mL 20.7125 mL 41.4250 mL 103.5626 mL
    5 mM 0.8285 mL 4.1425 mL 8.2850 mL 20.7125 mL
    10 mM 0.4143 mL 2.0713 mL 4.1425 mL 10.3563 mL
    15 mM 0.2762 mL 1.3808 mL 2.7617 mL 6.9042 mL
    20 mM 0.2071 mL 1.0356 mL 2.0713 mL 5.1781 mL
    25 mM 0.1657 mL 0.8285 mL 1.6570 mL 4.1425 mL
    30 mM 0.1381 mL 0.6904 mL 1.3808 mL 3.4521 mL
    40 mM 0.1036 mL 0.5178 mL 1.0356 mL 2.5891 mL
    50 mM 0.0829 mL 0.4143 mL 0.8285 mL 2.0713 mL
    60 mM 0.0690 mL 0.3452 mL 0.6904 mL 1.7260 mL
    80 mM 0.0518 mL 0.2589 mL 0.5178 mL 1.2945 mL
    100 mM 0.0414 mL 0.2071 mL 0.4143 mL 1.0356 mL
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    BLT-1 Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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    Product Name:
    BLT-1
    Cat. No.:
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