BODIPY FL SSE 

BODIPY FL SSE is a potent fluorescent dye. BODIPY FL SSE is used to label the primary amines (R-NH₂) of proteins, amine-modified oligonucleotides, and other amine-containing molecules. BODIPY FL SSE can reactive with primary amines on biomolecules to emit green fluorescence. (λ_ex=502 nm, λ_em=511 nm)^[1][2].

Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
BODIPY FL SSE assay (to quantify GAPDH)^[1]:
1.Make a 2 mg/ mL stock of GAPDH (MW 37 kDa) in PBS (pH 7.4).
2.Add 54 nM of BODIPY-FL-SSE to 1 mL of the 2 mg/ mL (54 μM) GAPDH solution. Incubate the mixture for 4 h on ice.
3.Dialyze the reaction mixture against 500 ml of PBS, pH 7.4, in a 10,000 MW-cutoff dialysis cassette for 1 h at 4 °C. Change the buffer and dialyze again overnight. Change the dialysis buffer once more the following morning and dialyze further for 1 h.
4.Run the dialyzed sample through a Zeba desalting column at 1000 g for 2 min.
5.Analyze the BD-GAPDH spectrophotometrically in a quartz cuvette. Set the wavelength scan to 250-700 nm. Blank the instrument against PBS and add the BD-GAPDH to the cuvette.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

491.21

C₁₈H₁₇BF₂N₃NaO₇S

217190-17-5

512

501

O=C(ON(C1=O)C(C(S(=O)(O[Na])=O)C1)=O)CCC2=CC=C(C=C(C(C)=C3)[N]4=C3C)[N-]2[B+3]4([F-])[F-]

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Hill BG, et, al. Methods for the determination and quantification of the reactive thiol proteome. Free Radic Biol Med. 2009 Sep 15;47(6):675-83.  [Content Brief]

  [2]. Gite S, et, al. Ultrasensitive fluorescence-based detection of nascent proteins in gels. Anal Biochem. 2000 Mar 15;279(2):218-25.  [Content Brief]