1. Dye Reagents
  2. BODIPY FL SSE

BODIPY FL SSE 

Cat. No.: HY-D1607
Handling Instructions

BODIPY FL SSE is a potent fluorescent dye. BODIPY FL SSE is used to label the primary amines (R-NH2) of proteins, amine-modified oligonucleotides, and other amine-containing molecules. BODIPY FL SSE can reactive with primary amines on biomolecules to emit green fluorescence. (λex=502 nm, λem=511 nm).

For research use only. We do not sell to patients.

BODIPY FL SSE Chemical Structure

BODIPY FL SSE Chemical Structure

CAS No. : 217190-17-5

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Description

BODIPY FL SSE is a potent fluorescent dye. BODIPY FL SSE is used to label the primary amines (R-NH2) of proteins, amine-modified oligonucleotides, and other amine-containing molecules. BODIPY FL SSE can reactive with primary amines on biomolecules to emit green fluorescence. (λex=502 nm, λem=511 nm)[1][2].

In Vitro

Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
BODIPY FL SSE assay (to quantify GAPDH)[1]:
1.Make a 2 mg/ mL stock of GAPDH (MW 37 kDa) in PBS (pH 7.4).
2.Add 54 nM of BODIPY-FL-SSE to 1 mL of the 2 mg/ mL (54 μM) GAPDH solution. Incubate the mixture for 4 h on ice.
3.Dialyze the reaction mixture against 500 ml of PBS, pH 7.4, in a 10,000 MW-cutoff dialysis cassette for 1 h at 4 °C. Change the buffer and dialyze again overnight. Change the dialysis buffer once more the following morning and dialyze further for 1 h.
4.Run the dialyzed sample through a Zeba desalting column at 1000 g for 2 min.
5.Analyze the BD-GAPDH spectrophotometrically in a quartz cuvette. Set the wavelength scan to 250-700 nm. Blank the instrument against PBS and add the BD-GAPDH to the cuvette.

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

491.21

Formula

C18H17BF2N3NaO7S

CAS No.
SMILES

O=C(ON(C1=O)C(C(S(=O)(O[Na])=O)C1)=O)CCC2=CC=C(C=C(C(C)=C3)[N]4=C3C)[N-]2[B+3]4([F-])[F-]

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation
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BODIPY FL SSE
Cat. No.:
HY-D1607
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