Brodimoprim
Based on 1 Customer Validation
Brodimoprim (Ro 10-5970), a trimethoprim analogue, is an orally active dihydrofolate reductase inhibitor. Brodimoprim is highly active against a broad spectrum of gram-negative and gram-positive bacteria.
For research use only. We do not sell to patients.
- Purity: 99.36%
- CAS No.: 56518-41-3
- Formula: C13H15BrN4O2
- Molecular Weight:339.19
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Storage:
-20°C, protect from light, stored under argon
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under argon)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Bioactive Compound Library Max
HY-L181
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Bioactive Compound Library
HY-L001
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Anti-Infection Compound Library
HY-L002
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Antibacterial Compound Library
HY-L049
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Orally Active Compound Library
HY-L061
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Heterocyclic Compound Library
HY-L138
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MCE Bioactive Compound Library
HY-L001V
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High-Throughput Bioactive Compound Library
HY-L205
Biological Activity
Chemical Information
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CAS No. 56518-41-3
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Appearance Solid
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Molecular Weight 339.19
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Formula C13H15BrN4O2
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Color White to yellow
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SMILES
COC1=C(C(OC)=CC(CC2=CN=C(N=C2N)N)=C1)Br
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Synonyms
Ro 10-5970
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
-20°C, protect from light, stored under argon
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under argon)
Solvent & Solubility
DMSO : 33.33 mg/mL (98.26 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under argon). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under argon). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (7.37 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under argon)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (271 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
[1]. Weidekamm E. Pharmacokinetics of brodimoprim. J Chemother. 1993;5(6):475-479. [Content Brief]
[2]. Marchese A, et al. Brodimoprim: effects of subminimal inhibitory concentrations on virulence traits of respiratory and urinary tract pathogens, and on plasmid transfer and stability. J Chemother. 1996;8(3):171-177. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under argon). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.9482 mL | 14.7410 mL | 29.4820 mL | 73.7050 mL |
| 5 mM | 0.5896 mL | 2.9482 mL | 5.8964 mL | 14.7410 mL | |
| 10 mM | 0.2948 mL | 1.4741 mL | 2.9482 mL | 7.3705 mL | |
| 15 mM | 0.1965 mL | 0.9827 mL | 1.9655 mL | 4.9137 mL | |
| 20 mM | 0.1474 mL | 0.7371 mL | 1.4741 mL | 3.6853 mL | |
| 25 mM | 0.1179 mL | 0.5896 mL | 1.1793 mL | 2.9482 mL | |
| 30 mM | 0.0983 mL | 0.4914 mL | 0.9827 mL | 2.4568 mL | |
| 40 mM | 0.0737 mL | 0.3685 mL | 0.7371 mL | 1.8426 mL | |
| 50 mM | 0.0590 mL | 0.2948 mL | 0.5896 mL | 1.4741 mL | |
| 60 mM | 0.0491 mL | 0.2457 mL | 0.4914 mL | 1.2284 mL | |
| 80 mM | 0.0369 mL | 0.1843 mL | 0.3685 mL | 0.9213 mL |
Brodimoprim Related Classifications
HY-121341 Related Classifications
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