BTK-IN-34
BTK-IN-34 (compound 9h) is a selective BTK inhibitor. BTK-IN-34 shows antiproliferative activity in RAMOS cells through selective inhibition of pBTK (Tyr223) without affecting Lyn and Syk, upstream proteins in the BCR signaling pathway.
For research use only. We do not sell to patients.
- CAS No.: 3016419-52-3
- Formula: C22H29N3O4S
- Molecular Weight:431.55
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
In Vitro
BTK-IN-34 (compound 9h; 1-50 μM; 24 hours) decreases the pBTK, pERK 1/2 (Thr202/Tyr204), and p-p38 (Thr180/Tyr182) levels in RAMOS cells[1].
BTK-IN-34 (compound 9h) displays selective cytotoxicity against BTK-high RAMOS cells with an IC50 of 2.75 μM.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
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Cell Line:RAMOS cells
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Concentration:1 μM, 10 μM, 50 μM
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Incubation Time:24 hours
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Result:Decreased pBTK (Tyr223) levels.
Chemical Information
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CAS No. 3016419-52-3
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Molecular Weight 431.55
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Formula C22H29N3O4S
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SMILES
O=C1NC2=C(C=C(S(=O)(N3CCN(C(CCCC)=O)CC3)=O)C=C2)/C1=C4CCCC\4
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocols
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Cell Viability Determination by MTT Colorimetric Assay
The following protocol uses the MTT colorimetric assay as a classic literature-established method for assessing cell viability/metabolic activity in cultured mammalian cells. MTT[3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] is reduced by metabolically active cells to a colored formazan product; the amount of formazan is quantified spectrophotometrically and provides an indirect measure of metabolically active viable cells. Importantly, MTT reduction reflects cellular oxidoreductase/metabolic activity rather than an absolute direct count of living cells, so changes in cellular metabolism can alter the signal independently of cell number.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
BTK-IN-34 Related Classifications
HY-162257 Related Classifications
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