Butyl caffeate
Based on 1 Customer Validation
Butyl caffeate (Caffeic acid butyl ester) is a caffeic acid derivative with antioxidant activity and lipophilicity (DPPH IC50 = 14.1 μM). Butyl caffeate protects neuronal PC12 cells against oxidative stress. Butyl caffeate can be used for oxidative stress related and neurodegenerative diseases research.
For research use only. We do not sell to patients.
- Purity : 98.15%
- CAS No.: 22020-28-6
- Formula: C13H16O4
- Molecular Weight:236.26
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Storage:
4°C, protect from light, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| A549 | IC50 |
3.51 μM
Compound: 2e
|
Cytotoxicity against human A549 cells after 72 hrs by alamar blue assay
Cytotoxicity against human A549 cells after 72 hrs by alamar blue assay
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[PMID: 22954735] |
| BeWo | IC50 |
5.42 μM
Compound: I-7
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Antitumor activity against human Bewo cells assessed as inhibition of cell growth by MTT assay
Antitumor activity against human Bewo cells assessed as inhibition of cell growth by MTT assay
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[PMID: 25160837] |
| Calu-1 | IC50 |
11.7 μM
Compound: 2e
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Cytotoxicity against human Calu1 cells after 72 hrs by alamar blue assay
Cytotoxicity against human Calu1 cells after 72 hrs by alamar blue assay
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[PMID: 22954735] |
| HeLa | IC50 |
0.59 μM
Compound: 2e
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Cytotoxicity against human HeLa cells after 72 hrs by alamar blue assay
Cytotoxicity against human HeLa cells after 72 hrs by alamar blue assay
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[PMID: 22954735] |
| HeLa | IC50 |
40.8 μM
Compound: I-7
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Antitumor activity against human HeLa cells assessed as inhibition of cell growth by MTT assay
Antitumor activity against human HeLa cells assessed as inhibition of cell growth by MTT assay
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[PMID: 25160837] |
| HepG2 | IC50 |
>100 μM
Compound: I-7
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Antitumor activity against human HepG2 cells assessed as inhibition of cell growth by MTT assay
Antitumor activity against human HepG2 cells assessed as inhibition of cell growth by MTT assay
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[PMID: 25160837] |
| HL-60 | IC50 |
7.54 μM
Compound: I-7
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Antitumor activity against human HL60 cells assessed as inhibition of cell growth by MTT assay
Antitumor activity against human HL60 cells assessed as inhibition of cell growth by MTT assay
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[PMID: 25160837] |
| HOP-62 | IC50 |
5.59 μM
Compound: 2e
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Cytotoxicity against human HOP62 cells after 72 hrs by alamar blue assay
Cytotoxicity against human HOP62 cells after 72 hrs by alamar blue assay
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[PMID: 22954735] |
| LNCaP | IC50 |
45.4 μM
Compound: 7
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Cytotoxicity against human LNCAP cells assessed as reduction in cell viability after 24 hrs by WST-1 assay
Cytotoxicity against human LNCAP cells assessed as reduction in cell viability after 24 hrs by WST-1 assay
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[PMID: 24080105] |
| LOX IMVI | IC50 |
9.54 μM
Compound: 2e
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Cytotoxicity against human LOXIMVI cells after 72 hrs by alamar blue assay
Cytotoxicity against human LOXIMVI cells after 72 hrs by alamar blue assay
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[PMID: 22954735] |
| M14 | IC50 |
11.1 μM
Compound: 2e
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Cytotoxicity against human M14 cells after 72 hrs by alamar blue assay
Cytotoxicity against human M14 cells after 72 hrs by alamar blue assay
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[PMID: 22954735] |
| NCI-H1299 | IC50 |
5.81 μM
Compound: 2e
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Cytotoxicity against human NCI-H1299 cells after 72 hrs by alamar blue assay
Cytotoxicity against human NCI-H1299 cells after 72 hrs by alamar blue assay
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[PMID: 22954735] |
| NCI-H157 | IC50 |
2.62 μM
Compound: 2e
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Cytotoxicity against human NCI-H157 cells after 72 hrs by alamar blue assay
Cytotoxicity against human NCI-H157 cells after 72 hrs by alamar blue assay
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[PMID: 22954735] |
| NCI-H1792 | IC50 |
5.75 μM
Compound: 2e
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Cytotoxicity against human NCI-H1792 cells after 72 hrs by alamar blue assay
Cytotoxicity against human NCI-H1792 cells after 72 hrs by alamar blue assay
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[PMID: 22954735] |
| NCI-H460 | IC50 |
5.62 μM
Compound: 2e
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Cytotoxicity against human H460 cells after 72 hrs by alamar blue assay
Cytotoxicity against human H460 cells after 72 hrs by alamar blue assay
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[PMID: 22954735] |
| RAW264.7 | EC50 |
0.24 μM
Compound: 5, butyl caffeate
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Inhibition of LPS-induced nitric oxide production in mouse RAW264.7 cells assessed as nitrite accumulation administered 1 hr before LPS challenge and measured after 24 hrs by Griess reagent assay
Inhibition of LPS-induced nitric oxide production in mouse RAW264.7 cells assessed as nitrite accumulation administered 1 hr before LPS challenge and measured after 24 hrs by Griess reagent assay
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[PMID: 18667320] |
| RAW264.7 | EC50 |
7.419 μM
Compound: 5, butyl caffeate
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Cytotoxicity against mouse RAW264.7 cells assessed as cell survival after 24 hrs by MTT assay
Cytotoxicity against mouse RAW264.7 cells assessed as cell survival after 24 hrs by MTT assay
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[PMID: 18667320] |
| SGC-7901 | IC50 |
>100 μM
Compound: I-7
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Antitumor activity against human SGC7901 cells assessed as inhibition of cell growth by MTT assay
Antitumor activity against human SGC7901 cells assessed as inhibition of cell growth by MTT assay
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[PMID: 25160837] |
| SiHa | IC50 |
40.97 μM
Compound: I-7
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Antitumor activity against human SiHa cells assessed as inhibition of cell growth by MTT assay
Antitumor activity against human SiHa cells assessed as inhibition of cell growth by MTT assay
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[PMID: 25160837] |
| WISH | IC50 |
~3 μM
Compound: 29
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In vitro inhibitory activity against the growth of WISH cell derived from human cervical carcinoma was determined
In vitro inhibitory activity against the growth of WISH cell derived from human cervical carcinoma was determined
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[PMID: 14684314] |
Chemical Information
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CAS No. 22020-28-6
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Appearance Solid
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Molecular Weight 236.26
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Formula C13H16O4
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SMILES
O=C(/C=C/C1=CC=C(C(O)=C1)O)OCCCC
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Synonyms
Caffeic acid butyl ester
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, protect from light, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
Solvent & Solubility
In Vitro:
DMSO : 100 mg/mL (423.26 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Protocols
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Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.
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ROS/oxidative-stress fluorescent staining
ROS/oxidative-stress fluorescent staining uses cell-permeant fluorogenic probes that become fluorescent after oxidation inside cells or tissues; commonly used examples include DCFH-DA/DCFDA for broad cellular oxidant detection, DHE for superoxide-related signal detection, MitoSOX for mitochondrial superoxide-related signal detection, and CellROX probes for oxidative-stress-associated fluorescence readouts. The assay detects probe oxidation rather than a single ROS species unless the probe and analysis method have been chemically validated for that species. DCFH-DA enters cells, is deacetylated by intracellular esterases to DCFH, and produces fluorescent DCF after oxidation, so the readout is used as an operational measure of total cellular oxidative stress rather than a species-specific ROS measurement. DHE and MitoSOX can report superoxide-related oxidation, but red fluorescence alone can include non-specific ethidium-like oxidation products; HPLC or optimized spectral approaches are
Purity & Documentation
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Data Sheet (266 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 4.2326 mL | 21.1631 mL | 42.3263 mL | 105.8156 mL |
| 5 mM | 0.8465 mL | 4.2326 mL | 8.4653 mL | 21.1631 mL | |
| 10 mM | 0.4233 mL | 2.1163 mL | 4.2326 mL | 10.5816 mL | |
| 15 mM | 0.2822 mL | 1.4109 mL | 2.8218 mL | 7.0544 mL | |
| 20 mM | 0.2116 mL | 1.0582 mL | 2.1163 mL | 5.2908 mL | |
| 25 mM | 0.1693 mL | 0.8465 mL | 1.6931 mL | 4.2326 mL | |
| 30 mM | 0.1411 mL | 0.7054 mL | 1.4109 mL | 3.5272 mL | |
| 40 mM | 0.1058 mL | 0.5291 mL | 1.0582 mL | 2.6454 mL | |
| 50 mM | 0.0847 mL | 0.4233 mL | 0.8465 mL | 2.1163 mL | |
| 60 mM | 0.0705 mL | 0.3527 mL | 0.7054 mL | 1.7636 mL | |
| 80 mM | 0.0529 mL | 0.2645 mL | 0.5291 mL | 1.3227 mL | |
| 100 mM | 0.0423 mL | 0.2116 mL | 0.4233 mL | 1.0582 mL |
Butyl caffeate Related Classifications
HY-N11229 Related Classifications
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