20-5,14-HEDE
Based on 1 Customer Validation
20-5,14-HEDE (WIT003) is an analog of 20-HETE. 20-5,14-HEDE activates PI3K/Akt signaling pathway, thereby exhibiting anti-apoptotic and cell survival promoting effects. 20-5,14-HEDE is the agonist for 20-HETE that increases intracellular Ca2+ concentrations, thereby enhancing vasoconstriction.
For research use only. We do not sell to patients.
- CAS No.: 240427-86-5
- Formula: C20H36O3
- Molecular Weight:324.50
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Storage:
4°C, protect from light, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
20-5,14-HEDE (0.1 nM-50 μM, 48 h) promotes the DNA synthesis in bovine pulmonary artery endothelial cells (BPAECs ), enhances the proliferation of BPAECs. 20-5,14-HEDE reduces serum starvation-induced caspase-3 activity and inhibits apoptosis in BPAECs. 20-5,14-HEDE increases ROS production in BPAECs[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:bovine pulmonary artery endothelial cells
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Concentration:1 μM
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Incubation Time:24 h
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Result:Reduced the cell viability.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Rat subarachnoid hemorrhage model[2]
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Dosage:1.5 nmol
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Administration:subarachnoid injection, single dose
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Result:Reduced basal cerebral blood flow.
Chemical Information
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CAS No. 240427-86-5
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Appearance Liquid (Density: 0.960±0.06 g/cm3)
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Molecular Weight 324.50
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Formula C20H36O3
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Color Colorless to light yellow
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SMILES
OCCCCC/C=C\CCCCCCC/C=C\CCCC(O)=O
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Synonyms
WIT003
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, protect from light, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
Solvent & Solubility
DMSO : ≥ 150 mg/mL (462.25 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 3.75 mg/mL (11.56 mM); Clear solution
This protocol yields a clear solution of ≥ 3.75 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (37.5 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (267 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
[1]. Dhanasekaran, et al. "20-HETE increases survival and decreases apoptosis in pulmonary arteries and pulmonary artery endothelial cells." American Journal of Physiology-Heart and Circulatory Physiology 296.3 (2009): H777-H786. [Content Brief]
[2]. Yu M, et al., Effects of a 20-HETE antagonist and agonists on cerebral vascular tone. Eur J Pharmacol. 2004 Feb 23;486(3):297-306. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.0817 mL | 15.4083 mL | 30.8166 mL | 77.0416 mL |
| 5 mM | 0.6163 mL | 3.0817 mL | 6.1633 mL | 15.4083 mL | |
| 10 mM | 0.3082 mL | 1.5408 mL | 3.0817 mL | 7.7042 mL | |
| 15 mM | 0.2054 mL | 1.0272 mL | 2.0544 mL | 5.1361 mL | |
| 20 mM | 0.1541 mL | 0.7704 mL | 1.5408 mL | 3.8521 mL | |
| 25 mM | 0.1233 mL | 0.6163 mL | 1.2327 mL | 3.0817 mL | |
| 30 mM | 0.1027 mL | 0.5136 mL | 1.0272 mL | 2.5681 mL | |
| 40 mM | 0.0770 mL | 0.3852 mL | 0.7704 mL | 1.9260 mL | |
| 50 mM | 0.0616 mL | 0.3082 mL | 0.6163 mL | 1.5408 mL | |
| 60 mM | 0.0514 mL | 0.2568 mL | 0.5136 mL | 1.2840 mL | |
| 80 mM | 0.0385 mL | 0.1926 mL | 0.3852 mL | 0.9630 mL | |
| 100 mM | 0.0308 mL | 0.1541 mL | 0.3082 mL | 0.7704 mL |
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