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Cat. No.: HY-15581

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MMAD

Name: MMAD
Brand: MedChemExpress
SKU: HY-15581
Price: 80.00 USD
Availability: InStock
Rating: 5 (1 reviews)

(Synonyms: Demethyldolastatin 10; Monomethylauristatin D; Monomethyl Dolastatin 10)

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MMAD is a potent tubulin inhibitor, is a toxin payload in antibody agent conjugates (ADCs).

For research use only. We do not sell to patients.

Purity: 98.94%
CAS No.: 203849-91-6
Formula: C₄₁H₆₆N₆O₆S
Molecular Weight:771.06

Storage:

4°C, stored under nitrogen

* The compound is unstable in solutions, freshly prepared is recommended.

MMAD Chemical Structure

CAS No.: 203849-91-6

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Biological Activity
- Description
- IC₅₀ & Target
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Solvent & Solubility
- In Vitro
Purity & Documentation
References
Help & FAQs
Classifications
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Biological Activity

Description

MMAD is a potent tubulin inhibitor, is a toxin payload in antibody agent conjugates (ADCs).

IC₅₀ & Target

Auristatin

All ADC Payloads Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
BT-474	GI₅₀	0.12 nM Compound: 2, MMAD	Cytotoxicity against human BT474 cells assessed as cell viability incubated fore 4 days by MTS assay Cytotoxicity against human BT474 cells assessed as cell viability incubated fore 4 days by MTS assay	[PMID: 25431858]
MDA-MB-361	GI₅₀	0.09 nM Compound: 2, MMAD	Cytotoxicity against human MDA-MB-361 cells assessed as cell viability incubated fore 4 days by MTS assay Cytotoxicity against human MDA-MB-361 cells assessed as cell viability incubated fore 4 days by MTS assay	[PMID: 25431858]
NCI-N87	GI₅₀	0.3 nM Compound: 2, MMAD	Cytotoxicity against human NCI-N87 cells assessed as cell viability incubated fore 4 days by MTS assay Cytotoxicity against human NCI-N87 cells assessed as cell viability incubated fore 4 days by MTS assay	[PMID: 25431858]

In Vitro

MMAD (Monomethyl Dolastatin 10) is coupled through a stable oxime-ligation process to yield several near-homogenous antibody-drug conjugates (ADCs) with a drug-to-antibody ratio of ~2.0. The resulting conjugates demonstrate good pharmacokinetic properties, potent in vitro cytotoxic activity against HER2+ cancer cells. When compared with ADCs prepared by cysteine alkylation following native interchain disulfide reduction, site-specific unnatural-amino-acid-based ADCs are shown to have increased in vitro cytotoxicity^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

The resulting antibody-drug conjugates (ADCs) demonstrate complete tumour regression in rodents. They also have an improved toxicology profile in rats^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS No. 203849-91-6
Appearance Solid
Molecular Weight 771.06
Formula C₄₁H₆₆N₆O₆S
Color White to off-white
SMILES

O=C([C@H](C)[C@H]([C@]1([H])CCCN1C(C[C@@H](OC)[C@H]([C@@H](C)CC)N(C([C@@H](NC([C@@H](N(C)[H])C(C)C)=O)C(C)C)=O)C)=O)OC)N[C@@H](CC2=CC=CC=C2)C3=NC=CS3
Synonyms

Demethyldolastatin 10; Monomethylauristatin D; Monomethyl Dolastatin 10
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, stored under nitrogen

* The compound is unstable in solutions, freshly prepared is recommended.

Solvent & Solubility

In Vitro:

DMSO : ≥ 100 mg/mL (129.69 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

* "≥" means soluble, but saturation unknown.

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

1.2969 mL

6.4846 mL

12.9692 mL

5 mM

0.2594 mL

1.2969 mL

2.5938 mL

10 mM

0.1297 mL

0.6485 mL

1.2969 mL

Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

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Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (274 KB)
SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Chudasama V, et al. Recent advances in the construction of antibody-drug conjugates. Nat Chem. 2016 Feb;8(2):114-9. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	1.2969 mL	6.4846 mL	12.9692 mL	32.4229 mL
	5 mM	0.2594 mL	1.2969 mL	2.5938 mL	6.4846 mL
	10 mM	0.1297 mL	0.6485 mL	1.2969 mL	3.2423 mL
	15 mM	0.0865 mL	0.4323 mL	0.8646 mL	2.1615 mL
	20 mM	0.0648 mL	0.3242 mL	0.6485 mL	1.6211 mL
	25 mM	0.0519 mL	0.2594 mL	0.5188 mL	1.2969 mL
	30 mM	0.0432 mL	0.2162 mL	0.4323 mL	1.0808 mL
	40 mM	0.0324 mL	0.1621 mL	0.3242 mL	0.8106 mL
	50 mM	0.0259 mL	0.1297 mL	0.2594 mL	0.6485 mL
	60 mM	0.0216 mL	0.1081 mL	0.2162 mL	0.5404 mL
	80 mM	0.0162 mL	0.0811 mL	0.1621 mL	0.4053 mL
	100 mM	0.0130 mL	0.0648 mL	0.1297 mL	0.3242 mL

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Keywords

MOQ

Minimum order quantity

100 mg

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Biological Activity
- Description
- IC₅₀ & Target
- In Vitro
- In Vivo
Chemical Information
Solvent & Solubility
- In Vitro
Purity & Documentation
References

Product Name:
Cat. No.:
Quantity:
MCE Japan Authorized Agent:

Product Name:
CAS No.:
Species:
Purity:
Quantity:

MMAD

Monomethyl auristatin E

MMAF

Paclitaxel

Dolastatin 10

PNU-159682

PF-06380101

Doxorubicin hydrochloride

Eribulin

Monomethyl auristatin E (Standard)

DXd

Mertansine

Monomethyl auristatin E (GMP)

All ADC Payloads Isoforms

Biological Activity

Chemical Information

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

Bioz

MMAD

Monomethyl auristatin E

MMAF

Paclitaxel

Dolastatin 10

PNU-159682

PF-06380101

Doxorubicin hydrochloride

Eribulin

Monomethyl auristatin E (Standard)

DXd

Mertansine

Monomethyl auristatin E (GMP)

All ADC Payloads Isoforms

Biological Activity

Chemical Information

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

MMAD Related Classifications

HY-15581 Related Classifications

Bioz