GCC-1
GCC-1 is a glycolipid. GCC-1 binds to CD1d to form a glycolipid-CD1d complex, which is presented to cellular TCR, thereby activating cells in a CD1d-dependent manner. GCC-1 induces Th1-biased cellular activation and IFN-γ production. GCC-1 can be used for research on colon adenocarcinoma.
For research use only. We do not sell to patients.
- Formula: C52H93NO10
- Molecular Weight:892.30
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
IC50 & Target
[1]|
CD1d |
In Vitro
GCC-1 (100 nM; 2-24 h) is more efficiently presented and persists longer on the surface of DC2.4 cells than αGalCer via CD1d-mediated antigen presentation[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
In Vivo
GCC-1 (1 nmol; i.v.; on days 3 and 10) induces potent antitumor activity, reducing tumor volume to approximately 1000 mm3 and prolonging survival[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:BALB/c mice[1]
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Dosage:1 nmol
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Administration:i.v.; on days 3 and 10
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Result:Reduced average tumor volume to approximately 1000 mm3 on day 30, whereas control groups exhibited tumor volumes close to 2000 mm3.
Significantly prolonged survival relative to αGalCer.
Chemical Information
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Molecular Weight 892.30
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Formula C52H93NO10
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SMILES
OC[C@H]([C@@H]([C@@H]([C@H]1O)O)O)O[C@@H]1OC[C@H](NC(CC2=CC=C(OCC3CCCCCCCCCCCCCCCCCC3)C=C2)=O)[C@H](O)[C@@H](CCCCCCCCCCCCCC)O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocols
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Naïve CD4+ T-cell subset differentiation/polarization
Naïve CD4+ T-cell subset differentiation/polarization is an in vitro assay in which purified naïve CD4+ T cells are activated through TCR and CD28 costimulation and cultured with defined cytokines and neutralizing antibodies to generate Th0, Th1, Th2, Th17, or induced Treg-like populations. Differentiation is detected by subset-associated cytokines and transcription factors: IFN-γ/T-bet for Th1, IL-4/GATA3 for Th2, IL-17A/RORγt for Th17, and Foxp3 for induced Treg cells. The assay readout is usually generated by intracellular cytokine staining after restimulation, transcription-factor staining by flow cytometry, ELISA of secreted cytokines, or gene-expression analysis. The result reflects cytokine-directed lineage commitment or polarization rather than antigen-specific immune protection by itself.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
GCC-1 Related Classifications
HY-189458 Related Classifications
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