4-Nitrophenyl phosphate disodium
Based on 1 publication(s) in Google Scholar
4-Nitrophenyl phosphate (p-Nitrophenyl phosphate) disodium is a non-proteinaceous chromogenic substrate for alkaline and acid phosphatases used in ELISA and conventional spectrophotometric assays.
For research use only. We do not sell to patients.
- Purity: 99.85%
- CAS No.: 4264-83-9
- Formula: C6H4NNa2O6P
- Molecular Weight:263.05
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Storage:
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) 4-Nitrophenyl phosphate disodium
More
Biological Activity
Guide (The following is our recommended protocol. This protocol is only a guide and should be modified according to your specific needs).
1. Equilibrate 4-Nitrophenyl phosphate to room temperature before opening.
2. Dissolve 10 mg of 4-Nitrophenyl phosphate in 10 mL of 1X Diethanolamine (HY-W035903) Substrate Buffer.
Note: Diethanolamine is effective at concentrations from 10 mM to 1 M.
3. Add 100 μL of the 4-Nitrophenyl phosphate solution to each microplate well.
4. Incubate plate at room temperature for 30 minutes, or until sufficient color develops.
5. Add 50 μL of 2 N NaOH to each well to stop the reaction.
6. Measure the absorbance at 405 nm. Alternatively, monitor reaction in a kinetic ELISA reader.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 4264-83-9
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Appearance Solid
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Molecular Weight 263.05
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Formula C6H4NNa2O6P
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Color White to yellow
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SMILES
O=P(O[Na])(O[Na])OC1=CC=C([N+]([O-])=O)C=C1
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Synonyms
p-Nitrophenyl phosphate disodium
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
Publications (1)
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Journal Impact Factor
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Most Recent
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Am J Physiol Cell Physiol
cGMP-dependent protein kinase II determines β-catenin accumulation that is essential for uterine decidualization in mice. [Abstract]2019 Dec 1;317(6):C1115-C1127. PMID: 31509448
Solvent & Solubility
H2O : 160 mg/mL (608.25 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (277 KB)
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SDS (640 KB)
- English - EN (640 KB)
- Français - FR (640 KB)
- Deutsch - DE (640 KB)
- Norwegian - NO (640 KB)
- Español - ES (640 KB)
- Swedish - SV (640 KB)
- Italian - IT (640 KB)
- Korean - KR (640 KB)
- Portuguese - PT (640 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O | 1 mM | 3.8016 mL | 19.0078 mL | 38.0156 mL | 95.0390 mL |
| 5 mM | 0.7603 mL | 3.8016 mL | 7.6031 mL | 19.0078 mL | |
| 10 mM | 0.3802 mL | 1.9008 mL | 3.8016 mL | 9.5039 mL | |
| 15 mM | 0.2534 mL | 1.2672 mL | 2.5344 mL | 6.3359 mL | |
| 20 mM | 0.1901 mL | 0.9504 mL | 1.9008 mL | 4.7519 mL | |
| 25 mM | 0.1521 mL | 0.7603 mL | 1.5206 mL | 3.8016 mL | |
| 30 mM | 0.1267 mL | 0.6336 mL | 1.2672 mL | 3.1680 mL | |
| 40 mM | 0.0950 mL | 0.4752 mL | 0.9504 mL | 2.3760 mL | |
| 50 mM | 0.0760 mL | 0.3802 mL | 0.7603 mL | 1.9008 mL | |
| 60 mM | 0.0634 mL | 0.3168 mL | 0.6336 mL | 1.5840 mL | |
| 80 mM | 0.0475 mL | 0.2376 mL | 0.4752 mL | 1.1880 mL | |
| 100 mM | 0.0380 mL | 0.1901 mL | 0.3802 mL | 0.9504 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
4-Nitrophenyl phosphate disodium Related Classifications
HY-15928 Related Classifications
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