12-Hydroxyfusicoccin
12-Hydroxyfusicoccin (12-hFC) is a Fusicoccin (HY-122815) derivative. 12-Hydroxyfusicoccin lacks antiproliferative activity and is inactive to protein-protein interactionin and protein synthesis in cancer cells.
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- CAS No.: 2281679-00-1
- Formule: C30H49NO8
- Masse moléculaire:551.71
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Stockage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Activité biologique
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Chemical Information
- Protocole
- Pureté et documentation
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Help & FAQs
Activité biologique
Description
In Vitro
12-hydroxyfusicoccin does not stabilize the 14-3-3ζ-GIGYF2 protein-protein interaction in HEK293 cells[1].
12-hydroxyfusicoccin (1 h) does not suppress global nascent protein synthesis in HEK293 cells[1].
12-hydroxyfusicoccin does not increase the binding of TTP to GIGYF2 in HEK293 cells[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
Chemical Information
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CAS No. 2281679-00-1
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Masse moléculaire 551.71
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Formule C30H49NO8
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SMILES
C[C@]12C([C@H]([C@@H]([C@@H]([C@@]3([H])/C([C@H](CC3)COC)=C\1)C)O)O[C@@H]4[C@@H]([C@]5([H])[C@](CO[C@H]5OCCCN)([H])O4)O)=C(C[C@@H]2O)C(C)C
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Synonyms
12-hFC
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocole
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Cell Viability Determination by MTT Colorimetric Assay
The following protocol uses the MTT colorimetric assay as a classic literature-established method for assessing cell viability/metabolic activity in cultured mammalian cells. MTT[3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] is reduced by metabolically active cells to a colored formazan product; the amount of formazan is quantified spectrophotometrically and provides an indirect measure of metabolically active viable cells. Importantly, MTT reduction reflects cellular oxidoreductase/metabolic activity rather than an absolute direct count of living cells, so changes in cellular metabolism can alter the signal independently of cell number.
Pureté et documentation
Références
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
12-Hydroxyfusicoccin Related Classifications
HY-181617 Related Classifications
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