Retro-2 cycl
Based on 1 publication(s) in Google Scholar
Retro-2 cycl (RN 1-001) is a retrograde transport inhibitor with antiviral activity. Retro-2 cycl (RN 1-001) inhibits JCPyV and HPV16 pseudoviruses, with an IC50 of 54 μM and 160 μM respectively.
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- Pureté: 99.17%
- CAS No.: 1429192-00-6
- Formule: C19H16N2OS
- Masse moléculaire:320.41
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Stockage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year
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Activité biologique
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Chemical Information
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Solvant et solubilité
- Pureté et documentation
- Références
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) Retro-2 cycl
More
Activité biologique
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| HeLa | EC50 |
27.3 μM
Compound: 1
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Cytoprotective activity against shiga toxin-induced cytotoxicity in human HeLa cells at 30 uM incubated for 4 hrs prior to shiga toxin addition measured after 20 hrs by liquid scintillation counting analysis
Cytoprotective activity against shiga toxin-induced cytotoxicity in human HeLa cells at 30 uM incubated for 4 hrs prior to shiga toxin addition measured after 20 hrs by liquid scintillation counting analysis
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[PMID: 23517565] |
| HeLa | EC50 |
27.3 μM
Compound: Retro-2; Cycl
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Inhibition of Escherichia coli Stx1 in human HeLa cells assessed as stimulation of protein synthesis by measuring increase in radiolabelled-methionine incorporation preincubated with cells for 20 mins followed by Stx1 addition and measured after 1 hr by s
Inhibition of Escherichia coli Stx1 in human HeLa cells assessed as stimulation of protein synthesis by measuring increase in radiolabelled-methionine incorporation preincubated with cells for 20 mins followed by Stx1 addition and measured after 1 hr by s
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[PMID: 32648758] |
Retro-2 cycl inhibits cellular infection by interfering with the formation of intracellular vacuoles in viruses, bacteria, or parasites[2].
Retro-2 cycl can effectively inhibit EV71 virus infection at a concentration of 12.56 μM in the cytopathic effect inhibition experiment, but when its concentration exceeds 500 μM, it shows lower cytotoxicity[3].
Retro-2 cycl (7.81-500 μM, 48h) demonstrates a dose-dependent effect on EV71-induced cytopathic effect in 293S cells, with a maximum suppression rate of 61.32±6.55%, an EC50 value of 12.56 μM, and antiviral activity represents by a selective index (SI) value greater than 39.81[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:293S
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Concentration:7.81, 15.63, 31.25, 62.5, 125, 250, 500 μM
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Incubation Time:48 h
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Result:Inhibited cell proliferation and CPE.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Newborn mice induced by EV71-WT[3]
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Dosage:2, 10, 50 mg/kg; daily; 7 days
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Administration:Intraperitoneal injection (i.p.)
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Result:Improved the survival rate of infected mice.
Chemical Information
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CAS No. 1429192-00-6
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Appearance Solid
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Masse moléculaire 320.41
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Formule C19H16N2OS
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Color White to off-white
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SMILES
O=C1C2=CC=CC=C2NC(N1C3=CC=CC=C3)C4=CC=C(S4)C
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Synonyms
RN 1-001
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 2 years -20°C 1 year
Publications (1)
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Journal Impact Factor
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Most Recent
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Nat Immunol
Type A cholesterol-dependent cytolysins translocate to the trans-Golgi network for NLRP3 inflammasome activation. [Abstract]2025 Oct;26(10):1673-1685. PMID: 40913097
Solvant et solubilité
DMSO : ≥ 125 mg/mL (390.13 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (6.49 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Pureté et documentation
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Fiche technique (280 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Instruction de manipulation (2659 KB)
Références
[1]. Carney DW, et al. Structural optimization of a retrograde trafficking inhibitor that protects cells from infections by human polyoma- and papillomaviruses. Bioorg Med Chem. 2014 Sep 1;22(17):4836-47. [Content Brief]
[2]. Wen Dai, et al. Antiviral Effect of Retro-2.1 against Herpes Simplex Virus Type 2 In Vitro. J Microbiol Biotechnol. 2018 Jun 28;28(6):849-859. [Content Brief]
[3]. Wenwen Dai, et al. Antiviral effects of Retro-2cycl and Retro-2.1 against Enterovirus 71 in vitro and in vivo. Antiviral Res. 2017 Aug:144:311-321. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.1210 mL | 15.6050 mL | 31.2100 mL | 78.0250 mL |
| 5 mM | 0.6242 mL | 3.1210 mL | 6.2420 mL | 15.6050 mL | |
| 10 mM | 0.3121 mL | 1.5605 mL | 3.1210 mL | 7.8025 mL | |
| 15 mM | 0.2081 mL | 1.0403 mL | 2.0807 mL | 5.2017 mL | |
| 20 mM | 0.1561 mL | 0.7803 mL | 1.5605 mL | 3.9013 mL | |
| 25 mM | 0.1248 mL | 0.6242 mL | 1.2484 mL | 3.1210 mL | |
| 30 mM | 0.1040 mL | 0.5202 mL | 1.0403 mL | 2.6008 mL | |
| 40 mM | 0.0780 mL | 0.3901 mL | 0.7803 mL | 1.9506 mL | |
| 50 mM | 0.0624 mL | 0.3121 mL | 0.6242 mL | 1.5605 mL | |
| 60 mM | 0.0520 mL | 0.2601 mL | 0.5202 mL | 1.3004 mL | |
| 80 mM | 0.0390 mL | 0.1951 mL | 0.3901 mL | 0.9753 mL | |
| 100 mM | 0.0312 mL | 0.1561 mL | 0.3121 mL | 0.7803 mL |
Retro-2 cycl Related Classifications
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