DBNBS
DBNBS is a radical scavenger. DBNBS undergoes non-radical nucleophilic addition to bind directly to cysteine (e.g., Cys-34 of BSA) to form a hydroxylamine, which is subsequently oxidized to produce an artifact spin adduct signal. DBNBS can be used for detecting biological free radicals.
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- CAS No.: 83016-63-1
- Formule: C6H3Br2NO4S
- Masse moléculaire:344.97
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Stockage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Activité biologique
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Chemical Information
- Pureté et documentation
- Références
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Help & FAQs
Activité biologique
DBNBS (2.5-5 mM; overnight) forms an artificial artifact nitroxide adduct with the Cys-34 thiol residue of BSA via non-radical nucleophilic addition followed by oxidation, whereas its tertiary carbon-centered radical adduct formed with photochemically oxidized BSA arises from genuine spin trapping[1].
DBNBS (2.5 mM; 1 min-2 h) rapidly and completely depletes the free Cys-34 thiol content of bovine serum albumin, with nearly complete depletion observable within 1 minute of incubation[1].
DBNBS (3.7 μM; 1 min-2 h) quenches the tryptophan fluorescence in bovine serum albumin through binding rather than chemical reactions, and exerts no effect on the fluorescence of free tryptophan under the tested conditions[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 83016-63-1
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Masse moléculaire 344.97
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Formule C6H3Br2NO4S
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SMILES
O=NC1=C(C=C(S(=O)(O)=O)C=C1Br)Br
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
Please store the product under the recommended conditions in the Certificate of Analysis.
Pureté et documentation
Références
[1]. Silvester JA, et al. A study of photochemically-generated protein radical spin adducts on bovine serum albumin: the detection of genuine spin-trapping and artefactual, non-radical addition in the same molecule. Redox report : communications in free radical research. 1997 Aug;3(4):225-31. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
DBNBS Related Classifications
HY-180497 Related Classifications
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