LysoTracker Red (solution)

Guide (The following is our recommended protocol. This protocol is only a guide and should be modified according to your specific needs).
Preparation of LysoTracker Red working solution
1. Working solution preparation:
1.1 Restore the solution to room temperature and concentrate it at the bottom of the tube by instantaneous centrifugation.
1.2 Dilute 1 mM storage solution to the working solution using a medium or appropriate buffer (such as PBS) . The recommended working solution is 50-100 nM;
Note: Please adjust the concentration of the LysoTracker Red working solution according to the actual situation, and use it now.
2. Cell staining
2.1 For suspension cells: Centrifuge at 1000 g at 4°C for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time.
For adherent cells: Discard the cell culture medium, and add trypsin to dissociate cells to make a single-cell suspension. Centrifuge at 1000 g at 4°C for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time.
2.2 Add 1 mL of LysoTracker Red working solution, and then incubate at room temperature for 5-30 minutes.
2.3 Centrifuge at 400 g at 4°C for 3-4 minutes and then discard the supernatant.
2.4 Wash twice with PBS, 5 minutes each time.
2.5 Resuspend cells with serum-free cell culture medium or PBS, and then detect by fluorescence microscope or flow cytometer.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.