Art. -Nr.: HY-117433

4-Hydroperoxy cyclophosphamide

13 Cited Publications

Kundenbewertung

Based on 13 publication(s) in Google Scholar

4-Hydroperoxy cyclophosphamide is the active metabolite form of the precursor Cyclophosphamide. 4-Hydroperoxy cyclophosphamide cross-links DNA to induce T cell apoptosis independent of caspase receptor activation, and can activate the mitochondrial death pathway by producing reactive oxygen species (ROS). 4-Hydroperoxy cyclophosphamide can be used in the study of rheumatoid arthritis and autoimmune diseases.

Nur für Forschungszwecke. Wir verkaufen nicht an Patienten.

Reinheit: 98.0%
CAS. Nr.: 39800-16-3
Formel: C₇H₁₅Cl₂N₂O₄P
Molecular Weight:293.08

Speicherung:

-20°C, stored under nitrogen

* In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)

4-Hydroperoxy cyclophosphamide Chemical Structure

CAS. Nr.: 39800-16-3

Größe

Preis

Verfügbarkeit

Menge

Amount: USD 0.00

* Bitte wählen Sie Menge, bevor Sie Artikel hinzufügen.

Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biologische Aktivität
- Beschreibung
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Publications (13)
Lösungsmittel & Löslichkeit
- In Vitro
- In Vivo
Reinheit & Dokumentation
Verweise
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

Cellular Effect

Cell Line	Type	Value	Description	References
L1210	IC₅₀	90 μM Compound: 4-HC	Concentration required to reduce the viability of L1210 cells by 50% after 1-h incubation at 37 degrees C Concentration required to reduce the viability of L1210 cells by 50% after 1-h incubation at 37 degrees C	[PMID: 1992116]
V79	IC₅₀	2.7 μM Compound: 4-hydroperoxycyclophosphamide	Antiproliferative activity against NTR expressing chinese hamster V79 cells after 72 hrs Antiproliferative activity against NTR expressing chinese hamster V79 cells after 72 hrs	[PMID: 16821793]
V79	IC₅₀	3.1 μM Compound: 4-hydroperoxycyclophosphamide	Antiproliferative activity against chinese hamster V79 cells after 72 hrs Antiproliferative activity against chinese hamster V79 cells after 72 hrs	[PMID: 16821793]

In Vitro

4-Hydroperoxy cyclophosphamide (1 or 3 μg/mL, 24, 48, 72 h) Apoptosis of Caspase-independent T cells is mediated by oxidative stress-induced mitochondrial apoptosis factor AIF and nuclear relocalization of EndoG^[1].
4-Hydroperoxy cyclophosphamide (1 μg/mL, 72, 96 h) Combines with methotrexate (HY-14519), RANKL expression was inhibited in IL-6/ SIL-6R-induced fibroblast-like synoviocytes by inhibiting the JAK2/STAT3 and p38MAPK signaling pathways^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[1]

Cell Line:

CTL
Concentration:

3 μg/mL
Incubation Time:

24, 48, 72 h
Result:

Increased the expression of p53 and Bax after 24 h.

Apoptosis Analysis^[1]

Cell Line:

Jurkat, CTL
Concentration:

1 or 3 μg/mL
Incubation Time:

24 , 48 , 72h
Result:

Caspase-8 deficiency prevented CD95-mediated death induced by anti-CD95 monoclonal antibody (mAb) APO-1.
Overexpression of Bcl-2 inhibited apoptosis.
Induced cell death proceeded independently of caspase inhibition.

In Vivo

4-Hydroperoxy cyclophosphamide (200 mg/kg intraperitoneally injected) kills Caspase-independent T cells and B cells in mice^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

BALB/c mice^[1]
Dosage:

200 mg/kg
Administration:

i.p.
Result:

Exhibited a marked depletion of immature double-positive CD4 þ CD8 þ thymocytes and mature single-positive CD4⁺ and CD8⁺ T cells.

Chemical Information

CAS. Nr. 39800-16-3
Appearance Solid
Molecular Weight 293.08
Formel C₇H₁₅Cl₂N₂O₄P
Color White to yellow
SMILES

O=P1(OCCC(N1)OO)N(CCCl)CCCl
Versand

Room temperature in continental US; may vary elsewhere.
Speicherung

-20°C, stored under nitrogen

* In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)

Publications (13)

All WBCell Proliferation/Viability AssayCell Imaging/StainingRT-PCR

Sort by:

Journal Impact Factor

Cell Death Discov

The EZH2-H3K27me3 axis modulates aberrant transcription and apoptosis in cyclophosphamide-induced ovarian granulosa cell injury. [Abstract]

2023 Nov 14;9(1):413. PMID: 37963880
Apoptosis

Bushen Jianpi Tiaoxue Decoction (BJTD) inhibits the LIF-mTOR signaling axis to regulate mitochondrial function and alleviate cyclophosphamide-induced diminished ovarian reserve. [Abstract]

2025 Jun;30(5-6):1331-1350. PMID: 40042747

4-Hydroperoxy cyclophosphamide purchased from MedChemExpress. Usage Cited in: Apoptosis. 2025 Jun;30(5-6):1331-1350. [Abstract]

Cell viability of KGN cells following treatment with various concentrations of 4-Hydroperoxy cyclophosphamide (4-HC, 0.01-64 μM) for 24 h (n = 3).

4-Hydroperoxy cyclophosphamide purchased from MedChemExpress. Usage Cited in: Apoptosis. 2025 Jun;30(5-6):1331-1350. [Abstract]

Significant induction of damage to KGN cells by the 6 µM concentration of 4-Hydroperoxy cyclophosphamide (4-HC) for 24 h.

4-Hydroperoxy cyclophosphamide purchased from MedChemExpress. Usage Cited in: Apoptosis. 2025 Jun;30(5-6):1331-1350. [Abstract]

Changes in LIF levels in KGN cells following 4-HC exposure for 24 h being measured by RT-qPCR experiments (n = 3). RT-qPCR analysis indicated a significant upregulation of LIF mRNA level in models compared to controls after 24 h of co-culture with 6 µM 4-Hydroperoxy cyclophosphamide (4-HC).
Free Radic Biol Med

S100A8 induces cyclophosphamide-induced alopecia via NCF2/NOX2-mediated ferroptosis. [Abstract]

2025 Mar 16:230:112-126. PMID: 39947495
Anal Chem

Exposome-Scale Investigation of Cl-/Br-Containing Chemicals Using High-Resolution Mass Spectrometry, Multistage Machine Learning, and Cloud Computing. [Abstract]

2025 Jun 3;97(21):11099-11109. PMID: 40401576

4-Hydroperoxy cyclophosphamide purchased from MedChemExpress. Usage Cited in: Anal Chem. 2025 Jun 3;97(21):11099-11109. [Abstract]

Western blot results for EZH2, H3K27me3, and γH2AX levels in the negative control (NC) and 4-Hydroperoxy cyclophosphamide (4-HC, 2.5 μM; 0.5-3 h) treatment groups. β-actin and H3 were blotted as loading controls. The results showed that with prolonged incubation time with 4-HC, the levels of H3K27me3 and EZH2 in primary GC cells decreased, while the level of γH2AX was significantly increased.
J Ethnopharmacol

Zuogui Wan alleviates intergenerational premature ovarian insufficiency through C9-mediated complement-coagulation cascade. [Abstract]

2026 Mar 1:358:120979. PMID: 41325864
J Ethnopharmacol

Discovery of petroleum ether extract of eclipta targeting p53/Fas pathway for the treatment of chemotherapy-induced alopecia: Network pharmacology and experimental validation. [Abstract]

2024 Oct 28:333:118405. PMID: 38844249
J Agric Food Chem

Optimized Extraction Process of Fu Brick Tea Polysaccharides Using Ultrasound-Assisted Enzymatic Strategy and Their Enhanced Antioxidant and Immunoprotective Activities. [Abstract]

2026 Jun 16. PMID: 42301720
Int J Cancer

Establishment of patient-derived organoids for guiding personalized therapies in breast cancer patients. [Abstract]

2024 Jul 15;155(2):324-338. PMID: 38533706
Biology (Basel)

Urolithin A Protects Ovarian Reserve Via Inhibiting PI3K/Akt Signaling and Preventing Chemotherapy-Induced Follicle Apoptosis. [Abstract]

2025 Jul 8;14(7):829. PMID: 40723388
Eng Comput

A global sensitivity analysis of a mechanistic model of neoadjuvant chemotherapy for triple negative breast cancer constrained by in vitro and in vivo imaging data. [Abstract]

2024;40(3):1469-1499. PMID: 39620056
Res Sq

2026 Jan 9.
bioRxiv

PAX3-FOXO1 drives targetable cell state-dependent metabolic vulnerabilities in rhabdomyosarcoma. [Abstract]

2025 Jan 19:2025.01.15.633227. PMID: 39868247
AfricArXiv Preprints

2212.

Lösungsmittel & Löslichkeit

In Vitro:

DMSO : 50 mg/mL (170.60 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

3.4120 mL

17.0602 mL

34.1204 mL

5 mM

0.6824 mL

3.4120 mL

6.8241 mL

10 mM

0.3412 mL

1.7060 mL

3.4120 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: 2.5 mg/mL (8.53 mM); Clear solution; Need ultrasonic

This protocol yields a clear solution of 2.5 mg/mL.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.5 mg/mL (8.53 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (8.53 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (280 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. Strauss G, et al. 4-hydroperoxy-cyclophosphamide mediates caspase-independent T-cell apoptosis involving oxidative stress-induced nuclear relocation of mitochondrial apoptogenic factors AIF and EndoG. Cell Death Differ. 2008 Feb;15(2):332-43. [Content Brief]

[2]. Niu HQ, et al. Combination of 4-hydroperoxy cyclophosphamide and methotrexate inhibits IL-6/sIL-6R-induced RANKL expression in fibroblast-like synoviocytes via suppression of the JAK2/STAT3 and p38MAPK signaling pathway. Int Immunopharmacol. 2018 Aug;61:45-53. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	3.4120 mL	17.0602 mL	34.1204 mL	85.3009 mL
	5 mM	0.6824 mL	3.4120 mL	6.8241 mL	17.0602 mL
	10 mM	0.3412 mL	1.7060 mL	3.4120 mL	8.5301 mL
	15 mM	0.2275 mL	1.1373 mL	2.2747 mL	5.6867 mL
	20 mM	0.1706 mL	0.8530 mL	1.7060 mL	4.2650 mL
	25 mM	0.1365 mL	0.6824 mL	1.3648 mL	3.4120 mL
	30 mM	0.1137 mL	0.5687 mL	1.1373 mL	2.8434 mL
	40 mM	0.0853 mL	0.4265 mL	0.8530 mL	2.1325 mL
	50 mM	0.0682 mL	0.3412 mL	0.6824 mL	1.7060 mL
	60 mM	0.0569 mL	0.2843 mL	0.5687 mL	1.4217 mL
	80 mM	0.0427 mL	0.2133 mL	0.4265 mL	1.0663 mL
	100 mM	0.0341 mL	0.1706 mL	0.3412 mL	0.8530 mL

Keywords

4-Hydroperoxy cyclophosphamide

13 Cited Publications

100 mg Angebot einholen

Subscribe to the

MedChemExpress

Newsletter

Produktname:
Art. -Nr.:
Menge:
MCE Japan Authorized Agent:

Produktname:
CAS. Nr.:
Species:
Reinheit:
Menge:

4-Hydroperoxy cyclophosphamide

MCE Bioactive Compound Library

Publications Citing Use of MedChemExpress (MCE) 4-Hydroperoxy cyclophosphamide

Biologische Aktivität

Chemical Information

Publications (13)

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

4-Hydroperoxy cyclophosphamide

MCE Bioactive Compound Library

Publications Citing Use of MedChemExpress (MCE) 4-Hydroperoxy cyclophosphamide

Biologische Aktivität

Chemical Information

Publications (13)

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

4-Hydroperoxy cyclophosphamide Related Classifications

HY-117433 Related Classifications