Art. -Nr.: HY-N0434

Astragaloside III

2 Cited Publications

Kundenbewertung

Based on 2 publication(s) in Google Scholar

Astragaloside III is a triterpenoid saponin. Astragaloside III can be extracted from Astragalus root. Astragaloside III increases NKG2D and induces TACE phosphorylation. Astragaloside III induces Apoptosis. Astragaloside III has antiviral activity against dengue serotypes 1 and 3. Astragaloside III has anticancer activity against breast and colon cancer.

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Reinheit: 99.70%
CAS. Nr.: 84687-42-3
Formel: C₄₁H₆₈O₁₄
Molecular Weight:784.97

Speicherung:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month

Astragaloside III Chemical Structure

CAS. Nr.: 84687-42-3

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Biologische Aktivität
- Beschreibung
- In Vitro
- In Vivo
Chemical Information
Publications (2)
Lösungsmittel & Löslichkeit
- In Vitro
- In Vivo
Reinheit & Dokumentation
Verweise
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

In Vitro

Astragaloside III (3.125-6.25 μg/mL) shows antiviral activity against dengue serotype 1 and serotype 3^[1].
Astragaloside III (4-40 nM) can significantly increase the expression of NKG2D and the production of IFN-γ in NK cells^[2].
Astragaloside III (10 μM; 8 h) significantly reduces the cell viability of bEnd.3 cells (about 10%)^[3].
Astragaloside III (10-100 ng/mL; 24-48 h) reduces the viability of MCF-7 breast cancer cells and increase the apoptosis rate^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[3]

Cell Line:

Mouse brain microvascular endothelial bEnd.3 cells
Concentration:

1000 nM
Incubation Time:

5 min, 15 min, 30 min, 60 min, 120 min
Result:

Induced phosphorylation of TACE.

In Vivo

Astragaloside III (50 mg/kg; i.v.; every 2 days; a total of 5 times) can significantly inhibit tumor growth and prolong survival time in CT26 tumor-bearing mice without obvious toxic side effects^[2].
Astragaloside III (10-20 mg/kg; i.p.; every 2 days) can inhibit the growth of MDA-MB-231 breast cancer xenografts in nude mice and increase tumor cell apoptosis^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Female BALB/c mice (6-8 weeks old) with CT26 colon cancer xenografts^[2]
Dosage:

50 mg/kg (mixed with 10% PEG-200 and 10% ethanol)
Administration:

Intravenous injection, once every 2 days, for 5 times
Result:

Significantly reduced tumor weight and volume.
Decreased the fold change of tumor volume increase, prolonged the survival rate (30% survival by day 50 vs. all dead in control by day 40).
Increased NK cell infiltration in tumors, and upregulated NKG2D, IFN-γ, and Fas expression in NK cells without obvious organ toxicity.

Chemical Information

CAS. Nr. 84687-42-3
Appearance Solid
Molecular Weight 784.97
Formel C₄₁H₆₈O₁₄
Color White to off-white
SMILES

O[C@@H]1[C@@H](O)[C@]([H])(O[C@@H]2[C@@H](O)[C@H](O)CO[C@]2(O[C@@H]3C(C)(C)[C@@]([C@@H](O)C[C@]4([H])[C@@]56CC[C@@]7(C)[C@@]4(C)C[C@H](O)[C@@]7([C@]8(C)O[C@H](C(C)(O)C)CC8)[H])([H])[C@]5(C6)CC3)[H])O[C@H](CO)[C@H]1O
Structure Classification
- Terpenoids
- Triterpenes
Initial Source
- Plants
- other families
Versand

Room temperature in continental US; may vary elsewhere.

Speicherung

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Publications (2)

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Journal Impact Factor

Phytother Res

Astragaloside III activates TACE/ADAM17-dependent anti-inflammatory and growth factor signaling in endothelial cells in a p38-dependent fashion. [Abstract]

2020 May;34(5):1096-1107. PMID: 32197276
Exp Cell Res

Network-based analysis with primary cells reveals drug response landscape of acute myeloid leukemia. [Abstract]

2020 Aug 1;393(1):112054. PMID: 32376287

Lösungsmittel & Löslichkeit

In Vitro:

DMSO : 50 mg/mL (63.70 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

1.2739 mL

6.3697 mL

12.7393 mL

5 mM

0.2548 mL

1.2739 mL

2.5479 mL

10 mM

0.1274 mL

0.6370 mL

1.2739 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (3.18 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: 2.5 mg/mL (3.18 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (3.18 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (286 KB)
SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. Indu P, et al. Antiviral activity of astragaloside II, astragaloside III and astragaloside IV compounds against dengue virus: Computational docking and in vitro studies. Microb Pathog. 2021 Mar;152:104563. [Content Brief]

[2]. Chen X, et al. Astragaloside III Enhances Anti-Tumor Response of NK Cells by Elevating NKG2D and IFN-γ. Front Pharmacol. 2019 Aug 13;10:898. [Content Brief]

[3]. Wang H, et al. Astragaloside III activates TACE/ADAM17-dependent anti-inflammatory and growth factor signaling in endothelial cells in a p38-dependent fashion. Phytother Res. 2020 May;34(5):1096-1107. [Content Brief]

[4]. Wang S, et al. Astragaloside III from Astragalus membranaceus antagonizes breast cancer growth[J]. African Journal of Traditional, Complementary and Alternative Medicines, 2015, 12(3): 183-186.

[5]. SHI Jing-chao, et al. Determination of Astragaloside III and Astragaloside IV in Astragalus with HPLC-ELSD Method. World Journal of Integrated Traditional and Western Medicine, 2014-07

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	1.2739 mL	6.3697 mL	12.7393 mL	31.8484 mL
	5 mM	0.2548 mL	1.2739 mL	2.5479 mL	6.3697 mL
	10 mM	0.1274 mL	0.6370 mL	1.2739 mL	3.1848 mL
	15 mM	0.0849 mL	0.4246 mL	0.8493 mL	2.1232 mL
	20 mM	0.0637 mL	0.3185 mL	0.6370 mL	1.5924 mL
	25 mM	0.0510 mL	0.2548 mL	0.5096 mL	1.2739 mL
	30 mM	0.0425 mL	0.2123 mL	0.4246 mL	1.0616 mL
	40 mM	0.0318 mL	0.1592 mL	0.3185 mL	0.7962 mL
	50 mM	0.0255 mL	0.1274 mL	0.2548 mL	0.6370 mL
	60 mM	0.0212 mL	0.1062 mL	0.2123 mL	0.5308 mL

Keywords

Astragaloside III

2 Cited Publications

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Astragaloside III

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Publications Citing Use of MedChemExpress (MCE) Astragaloside III

Biologische Aktivität

Chemical Information

Publications (2)

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

Astragaloside III Related Classifications

HY-N0434 Related Classifications