Art. -Nr.: HY-B2156

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Menaquinone-4

(Synonyms: Vitamin K2(MK-4); Menaquinone K4)

8 Cited Publications

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Based on 8 publication(s) in Google Scholar

Menaquinone-4 is a vitamin K, used as a hemostatic agent, and also a adjunctive therapy for the pain of osteoporosis.

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Reinheit: 99.96%
CAS. Nr.: 863-61-6
Formel: C₃₁H₄₀O₂
Molecular Weight:444.65

Speicherung:

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Menaquinone-4 Chemical Structure

CAS. Nr.: 863-61-6

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Use of MCE Products

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Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biologische Aktivität
- Beschreibung
- IC₅₀ & Target
- In Vitro
- In Vivo
Chemical Information
Publications (8)
Lösungsmittel & Löslichkeit
- In Vitro
- In Vivo
Protokoll
Reinheit & Dokumentation
Verweise
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

Menaquinone-4 is a vitamin K, used as a hemostatic agent, and also a adjunctive therapy for the pain of osteoporosis.

IC₅₀ & Target

Human Endogenous Metabolite

Alle Endogenous Metabolite Isoform-spezifische Produkte anzeigen :

In Vitro

Menaquinone-4 (MK-4, 0, 1, 5, 10 μM) increases the ALP activity in Caco-2 cells. Menaquinone-4 (1 μM) significantly increases intensities of hSI expression^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Menaquinone-4 (K2, 0.2 g/kg diet) in HF-K2 group produces epididymal fat in C57BL/6J mice, and also increases the bone density of mice^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS. Nr. 863-61-6
Appearance <35°C Solid,>35°C Liquid
Molecular Weight 444.65
Formel C₃₁H₄₀O₂
Color Light yellow to yellow
SMILES

O=C1C(C)=C(C/C=C(C)/CC/C=C(C)/CC/C=C(C)/CC/C=C(C)\C)C(C2=C1C=CC=C2)=O
Synonyms

Vitamin K2(MK-4); Menaquinone K4
Structure Classification
- Quinones
- Naphthalene Quinones
Initial Source
- Plants
- other families
- Microorganisms
- Endogenous metabolite
Versand

Room temperature in continental US; may vary elsewhere.
Speicherung

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Publications (8)

All IFELISAIPCell Proliferation/Viability Assay

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Journal Impact Factor

J Pharm Anal

Screen of FDA-approved drug library identifies vitamin K as anti-ferroptotic drug for osteoarthritis therapy through Gas6. [Abstract]

2025 May;15(5):101092. PMID: 40496065

Menaquinone-4 purchased from MedChemExpress. Usage Cited in: J Pharm Anal. 2025 May;15(5):101092. [Abstract]

Menaquinone-4 (VK2, 0.5-40 μM; 24 h). Evaluation of cellular toxicity of vitamin K. Chondrocytes were treated with indicated vitamin K concentrations for 24 h.
Acta Pharmacol Sin

Ginsenoside (20)S-APPT induces ferroptosis in hepatocellular carcinoma and cholangiocarcinoma by targeting FSP1. [Abstract]

2025 Jun 23. PMID: 40550962

Menaquinone-4 purchased from MedChemExpress. Usage Cited in: Acta Pharmacol Sin. 2025 Jun 23. [Abstract]

Cell viability analysis of SK-HEP-1 cells after treatment with 10 μM (20)S-APPT alone or simultaneously with various VKs (VK1 at 10 μM, Menaquinone-4 (VK2) at 10 μM, VK3 at 5 μM, and MK4 at 5 μM). The results demonstrated that VK2 could also serve as a substrate for FSP1, significantly attenuating cell death induced by (20)S-APPT.
J Ethnopharmacol

Ginkgo biloba extract GBE50 alleviates vascular calcification through inhibiting the NF-κB pathway and the NLRP3 inflammasome. [Abstract]

2026 Oct 28:369:121880. PMID: 42155821
Int Immunopharmacol

Menaquinone-4 alleviates hypoxic-ischemic brain damage in neonatal rats by reducing mitochondrial dysfunction via Sirt1-PGC-1α-TFAM signaling pathway. [Abstract]

2024 May 16:134:112257. PMID: 38759366
Int J Antimicrob Agents

SU3327: A multi-target compound targeting bacterial menaquinone and DNA. [Abstract]

2025 Nov 25;67(1):107679. PMID: 41308844
J Inflamm Res

Menaquinone-4 Alleviates Sepsis-Associated Acute Lung Injury via Activating SIRT3-p53/SLC7A11 Pathway. [Abstract]

2024 Oct 24:17:7675-7685. PMID: 39469061

Menaquinone-4 purchased from MedChemExpress. Usage Cited in: J Inflamm Res. 2024 Oct 24:17:7675-7685. [Abstract]

Immunofluorescence staining and quantitative detection of ROS levels in lung tissue. Magnification: ×200 (n=3). The results showed that the ROS level was significantly increased in the sepsis group (CLP group) compared with the control group or the sham-operated group. However, the ROS level in the Menaquinone-4 (MK-4, 200 mg/kg; i.p.; once daily for 3 days) + CLP group was markedly lower than that in the CLP group, suggesting that MK-4 ameliorated oxidative stress in SI-ALI in C57BL/6N mice.CLP: Cecal ligation and puncture.

Menaquinone-4 purchased from MedChemExpress. Usage Cited in: J Inflamm Res. 2024 Oct 24:17:7675-7685. [Abstract]

ELISA was used to measure the levels of IL-6 and TNF-α in cardiac blood (n=3). The results indicated that Menaquinone-4 (MK‑4, 200 mg/kg; i.p.; once daily for 3 days) reduced the levels of inflammatory cytokines in SI‑ALI in C57BL/6N mice. CLP: Cecal ligation and puncture.

Menaquinone-4 purchased from MedChemExpress. Usage Cited in: J Inflamm Res. 2024 Oct 24:17:7675-7685. [Abstract]

Menaquinone-4 (MK-4, 200 mg/kg; i.p.; once daily for 3 days) attenuated cecal ligation and puncture (CLP)-induced p53 acetylation in C57BL/6N mice.
Gene

Menaquinone-4 ameliorates abdominal aortic aneurysm by suppressing ferroptosis through NRF2/GCLM axis. [Abstract]

2025 Oct 20:970:149785. PMID: 40992546
SSRN

2024 Nov 1.

Lösungsmittel & Löslichkeit

In Vitro:

DMSO : 10 mg/mL (22.49 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.2490 mL

11.2448 mL

22.4896 mL

5 mM

0.4498 mL

2.2490 mL

4.4979 mL

10 mM

0.2249 mL

1.1245 mL

2.2490 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.08 mg/mL (4.68 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: 2 mg/mL (4.50 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 2 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2 mg/mL (4.50 mM); Clear solution

This protocol yields a clear solution of ≥ 2 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Protokoll

Cell Assay^[1]

Caco-2 cells are plated at a density of 2-5 × 10⁴ cells/cm² onto a 35-mm dish. Cells are incubated for 2 to 3 days until 60%-70% confluency, and desired concentrations of Menaquinone-4 (0, 1.0, 5.0, and 10.0 μM) are added. The final concentration of the vehicle is 0.1% of the culture medium, and the culture medium is changed twice a week. Cells are assayed on days 0, 3, 7, and 11 after the addition of Menaquinone-4^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Administration^[2]

Forty-two male, 4-week-old C57BL/6J mice provided with feed and drink ad libitum. For environmental adaptation, the animals had 1 week of circulation, and then are provided with the experimental diet after being randomly divided into 6 groups (7 animals in each group; randomized block design). The AIN-93G diets consist of a normal diet (N), normal diet + vitamin K1 (N-K1), normal diet + vitamin Menaquinone-4 (N-K2), 45% high-fat diet (HF), 45% high-fat diet + vitamin K1 (HF-K1), and a 45% high-fat diet + vitamin Menaquinone-4 (HF-K2). The vitamin K1 and vitamin Menaquinone-4 contents are 200 mg/1,000 g, and the diet is provided in pellet form. Body weight is measured once a week, and the food efficiency ratio (FER) is calculated by dividing the increased body weight from day 1 to the final day by the food intake amount during the experiment period. For fat amount measurement, the epididymal fat, perirenal fat, and retroperitoneal fat are extracted from dead animal subjects, are washed with 0.9% NaCl, dried by filter paper, and then are weighed^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (271 KB)
SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. Noda S, et al. Menaquinone-4 (vitamin K2) up-regulates expression of human intestinal alkaline phosphatase in Caco-2 cells. Nutr Res. 2016 Nov;36(11):1269-1276. [Content Brief]

[2]. Kim M, et al. Vitamin K1 (phylloquinone) and K2 (menaquinone-4) supplementation improves bone formation in a high-fat diet-induced obese mice. J Clin Biochem Nutr. 2013 Sep;53(2):108-13. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.2490 mL	11.2448 mL	22.4896 mL	56.2240 mL
	5 mM	0.4498 mL	2.2490 mL	4.4979 mL	11.2448 mL
	10 mM	0.2249 mL	1.1245 mL	2.2490 mL	5.6224 mL
	15 mM	0.1499 mL	0.7497 mL	1.4993 mL	3.7483 mL
	20 mM	0.1124 mL	0.5622 mL	1.1245 mL	2.8112 mL

Keywords

Menaquinone-4

8 Cited Publications

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Menaquinone-4

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Publications Citing Use of MedChemExpress (MCE) Menaquinone-4

Alle Endogenous Metabolite Isoform-spezifische Produkte anzeigen

Biologische Aktivität

Chemical Information

Publications (8)

Lösungsmittel & Löslichkeit

Protokoll

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

Menaquinone-4 Related Classifications

HY-B2156 Related Classifications