TOK-8801

Spleen cells (2×10⁵) or thymus cells (2×10⁵) from BALB/c mice are cultured with Con A (1 or 5 μg/mL) or LPS (10 μg/mL) in 0.2 mL of RPMI-1640 medium containing 5% FCS using round-bottomed microculture plates with 96 wells for 2 days and are pulsed with 0.5 μCi of ³H-TdR for an additional 18 hr. Then, cells are harvested on glass fiber fillters, and ³H-TdR incorporation is measured in a liquid scintillation counter. TOK-8801 is added at the initiation of the culture^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Male BDFI mice are used in this study. Control mice are maintained without food or water while counterparts are under the condition of restraint-stress. On the next day, the mice are immunized with 5×10⁸ SRBC intraperitoneally. Immediately, 24 hr and 48 hr after the immunization, TOK-8801 is orally given at doses of 0.02, 0.1 and 0.5 mg/kg/day. All administrations are done after a starvation for 6 hr. Four days after the immunization, splenic anti SRBC PFC are assayed^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.