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Art. -Nr.: HY-D3404

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BODi-1

BODi-1 is a fluorescent modulator targeting dsDNA, which binds to dsDNA via a bis-intercalation mechanism (E_x=465 nm, E_m=490 nm). BODi-1 exhibits a fluorescence enhancement effect upon binding to nucleic acids, but its fluorescence intensity, anisotropy and average lifetime decrease at higher dye/DNA ratios. When BODi-1 binds to DNA in liposome complexes, it also shows red-shifted emission spectra, along with reduced quantum yield and average lifetime. BODi-1 does not induce significant DNA conformational changes when the dye/DNA ratio is below 0.01. BODi-1 can be used as a fluorescent probe for the characterization of liposome complexes and FRET studies at this ratio.

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CAS. Nr.: 169454-13-1
Formel: C₄₁H₅₄I₄N₆S₂
Molecular Weight:1202.66

Speicherung:

Please store the product under the recommended conditions in the Certificate of Analysis.

BODi-1 Chemical Structure

CAS. Nr.: 169454-13-1

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Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biologische Aktivität
- Beschreibung
- In Vitro
Chemical Information
Reinheit & Dokumentation
Verweise
Calculators
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

In Vitro

BOBO-1 exhibits stable fluorescence intensity and anisotropy (~0.20) in supercoiled and relaxed pUC19 DNA when the dye/DNA base ratio ≤ 0.01^[1].
BOBO-1 maintains stable fluorescence anisotropy (~0.20) in pUC19 DNA/DOTAP lipid complexes when the dye/DNA base ratio is ≤0.005; at higher ratios, its fluorescence intensity and anisotropy decrease^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS. Nr. 169454-13-1
Molecular Weight 1202.66
Formel C₄₁H₅₄I₄N₆S₂
SMILES

C[N+]1=C(/C=C2C=CN(CCC[N+](C)(C)CCC[N+](C)(C)CCCN3C=C/C(C=C3)=C\C4=[N+](C)C5=C(C=CC=C5)S4)C=C\2)SC6=C1C=CC=C6.[I-].[I-].[I-].[I-]
Versand

Room temperature in continental US; may vary elsewhere.
Speicherung

Please store the product under the recommended conditions in the Certificate of Analysis.

Reinheit & Dokumentation

Handling Instructions (2659 KB)

Verweise

[1]. Madeira C, et al. Photophysical behavior of a dimeric cyanine dye (BOBO-1) within cationic liposomes. Photochem Photobiol. 2005;81(6):1450-1459. [Content Brief]

Calculators

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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100 mg

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