ERNT
ERNT is a fluorescent probe for monitoring endoplasmic reticulum (ER) polarity and detecting endoplasmic reticulum stress (ERS) during the progression of dynamic liver injury. ERNT functions based on a donor-π-acceptor (D-π-A) structure with an intramolecular charge transfer (ICT) effect. ERNT has multiple excitation/emission pairs: for in vitro cell imaging, the green channel has Ex/Em = 488/500-550 nm, and the red channel has Ex/Em = 488/570-620 nm; for in vivo liver imaging, Ex/Em = 520/620 nm; for solvent optical tests, Ex = 470 nm, with the maximum emission peak at 548 nm in low-polarity toluene and 668 nm in high-polarity DMSO. ERNT can evaluate the efficacy of hepatoprotective interventions by detecting changes in ER polarity, and also exhibits excellent photostability and low cytotoxicity.
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- CAS. Nr.: 2767443-23-0
- Formel: C39H31N3O4S
- Molecular Weight:637.75
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Speicherung:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biologische Aktivität
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Chemical Information
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Help & FAQs
Biologische Aktivität
Beschreibung
In Vitro
Guideline
(The following is a recommended experimental protocol for guidance only, and adjustments are required based on your specific needs)[1].
1. Preparation of Working Solution
1.1 Concentration recommendations: 10 μM; 15 μM.
2. Staining Procedures
2.1 Sample types:
2.1.1 Adherent cells (HepG2).
2.1.2 Liver tissue samples: Isolate the liver organ and rinse it with PBS (pH 7.4).
2.2 Incubation conditions:
2.2.1 Adherent cells: Incubate with 10 μM ERNT (with continuous irradiation for 0, 5, 10, 15 and 20 min).
2.2.2 Liver tissue samples: Incubate with 15 μM ERNT.
2.3 Washing procedures:
2.3.1 Liver tissue samples: Rinse three times with PBS (pH 7.4) before staining.
3. Control Group Setup
3.1 Set up untreated adherent cells as the control group.
3.2 Set up untreated liver-injured mice as the control group.
3.3 Set up normal liver tissue as the reference group.
4. Detection and Analysis
4.1 Instruments: Fluorescence imaging system; In vivo imaging system.
4.1.1 Adherent cells: Green channel: Ex = 488 nm, Em = 500-550 nm; Red channel: Ex = 488 nm, Em = 570-620 nm.
4.1.2 Liver tissue samples: Ex = 520 nm, Em = 620 nm; Ex = 570 nm, Em = 710 nm.
4.2 Result analysis:
4.2.1 Changes in fluorescence intensity:
4.2.1.1 Higher fluorescence intensity indicates lower endoplasmic reticulum (ER) polarity; lower fluorescence intensity indicates higher ER polarity.
4.2.1.2 Fluorescence intensity decreases gradually from normal liver tissue to fatty liver, liver fibrosis, liver cirrhosis and finally liver cancer.
4.2.1.3 Compared with the untreated control group, the fluorescence intensity of the drug-treated liver injury group increases.
4.2.1.4 The fluorescence intensity ratio of green channel to red channel (Igreen/Ired) in normal cells is higher than that in ER stress-induced cells.
4.2.2 Fluorescence localization: Mainly localized to the endoplasmic reticulum.
4.2.3 Color changes: Emits green and red fluorescence in cells; emits yellow to red fluorescence in liver tissue.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS. Nr. 2767443-23-0
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Molecular Weight 637.75
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Formel C39H31N3O4S
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SMILES
CC1=CC=C(C=C1)S(=O)(NCCN2C(C3=CC=C(C4=CC=CC(C2=O)=C34)C5=CC=C(C=C5)N(C6=CC=CC=C6)C7=CC=CC=C7)=O)=O
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Versand
Room temperature in continental US; may vary elsewhere.
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Speicherung
Please store the product under the recommended conditions in the Certificate of Analysis.
Reinheit & Dokumentation
Verweise
Calculators
Konzentration (Stammlösung) × Volumen (Stammlösung) = Konzentration (Ziellösung) × Volumen (Ziellösung)
ERNT Related Classifications
HY-D3104 Related Classifications
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