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Art. -Nr.: HY-171036

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GAPDH-IN-1

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GAPDH-IN-1 (Compound F8) is a GAPDH inhibitor (IC₅₀ of 39.31 μM for GAPDH enzymatic activity). GAPDH-IN-1 forms a covalent adduct with an aspartic acid in the active site to displace NAD⁺, a cofactor of the enzyme, with concomitant enhancement of the cysteine-reactive probe reaction with the catalytic cysteine.

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Reinheit: 98.10%
CAS. Nr.: 252212-58-1
Formel: C₂₀H₁₈N₂O₅
Molecular Weight:366.37

Speicherung:

Powder -20°C, 3 years ; In solvent -80°C, 6 months , -20°C, 1 month

GAPDH-IN-1 Chemical Structure

CAS. Nr.: 252212-58-1

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Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biologische Aktivität
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- In Vitro
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Biologische Aktivität

Beschreibung

Alle Endogenous Metabolite Isoform-spezifische Produkte anzeigen :

In Vitro

GAPDH-IN-1 (1.6-50 μM) exhibits a dose-dependent increase in the probe labeling of the 40 kDa protein^[1].
GAPDH-IN-1 (50 μM, 4 h) covalently reacts with Asp35 but not cysteine residues in GAPDH, stimulating the subsequent reaction of Cys152 with the iodoacetamide-based (IA) probe^[1].
GAPDH-IN-1 (25-50 μM, 24 h) enhances probe and GAPDH responses less in HEK293 cells than in cell lysates^[1].
GAPDH-IN-1 (0-100 μM, 24 h) displays a concentration-dependent inactivation of GAPDH in HEK293 cell lysates, with an IC₅₀ of 39.31 μM^[1].
GAPDH-IN-1 (0-100 μM) inhibits the viability of HEK293 cells, with an IC₅₀ of 50.64 μM^[1].
GAPDH-IN-1-GAPDH adduct can be reduced by the addition of excess NAD⁺, as evidenced by the band shifts of endogenous GAPDH in a silver-stained gel^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[1]

Cell Line:

HEK293 cells transiently expressing FLAG-tagged GAPDH or its cysteine-to-alanine mutants
Concentration:

50 μM
Incubation Time:

4 h
Result:

The band of FLAG-GAPDH was slightly shifted.
Showed band shifts in all the cysteine mutants (C152A, C156A, and C247A).

Chemical Information

CAS. Nr. 252212-58-1
Appearance Solid
Molecular Weight 366.37
Formel C₂₀H₁₈N₂O₅
Color White to off-white
SMILES

C1(C2=CC(OCC3OC3)=CC=C2)=NN=C(C4=CC(OCC5OC5)=CC=C4)O1
Versand

Room temperature in continental US; may vary elsewhere.

Speicherung

Powder	-20°C	3 years
In solvent	-80°C	6 months
	-20°C	1 month

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (269 KB)
SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. Jung Y, et al. Discovery of Non-Cysteine-Targeting Covalent Inhibitors by Activity-Based Proteomic Screening with a Cysteine-Reactive Probe. ACS Chem Biol. 2022 Feb 18;17(2):340-347. [Content Brief]

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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GAPDH-IN-1

Anti-Cancer Metabolism Compound Library

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Alle Endogenous Metabolite Isoform-spezifische Produkte anzeigen

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GAPDH-IN-1

Anti-Cancer Metabolism Compound Library

Extracellular Vesicles (EVs) Compound Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Bioactive Compound Library

High-Throughput Bioactive Compound Library

Lactylation Compound Library

Alle Endogenous Metabolite Isoform-spezifische Produkte anzeigen

Biologische Aktivität

Chemical Information

Reinheit & Dokumentation

Verweise

Calculators

Help & FAQs

GAPDH-IN-1 Related Classifications

HY-171036 Related Classifications