Glucolimnanthin
Glucolimnanthin is a plant glucoside that can be isolated from Limnanthes douglasii. Glucolimnanthin can increase metabolic activation of benzo[a]pyrene (B[a]P) in cultured mammalian cells
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- CAS. Nr.: 111810-95-8
- Formel: C15H21NO10S2
- Molecular Weight:439.46
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Speicherung:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biologische Aktivität
Beschreibung
Chemical Information
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CAS. Nr. 111810-95-8
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Molecular Weight 439.46
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Formel C15H21NO10S2
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SMILES
COC1=CC=CC(C/C(S[C@@H]2O[C@@H]([C@H]([C@@H]([C@H]2O)O)O)CO)=N\OS(=O)(O)=O)=C1
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Structure Classification
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Initial Source
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Versand
Room temperature in continental US; may vary elsewhere.
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Speicherung
Please store the product under the recommended conditions in the Certificate of Analysis.
Protokoll
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CRISPR-Cas9 knockout in cultured mammalian cells
CRISPR-Cas9 knockout in cultured mammalian cells uses an sgRNA to direct Cas9 to a complementary genomic sequence adjacent to a compatible PAM; Cas9 creates a targeted DNA double-strand break, and repair by non-homologous end joining can introduce insertions or deletions that disrupt the coding sequence or functional genomic element. The readout of knockout is detection of edited alleles and loss of gene product or phenotype, commonly by PCR/Sanger-sequence trace decomposition, targeted sequencing, immunoblotting, immunostaining, or flow cytometry when the target protein is detectable at the cell surface.
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Detergent-Based Total Protein Extraction from Cultured Mammalian Cells (RIPA/NP-40/Triton Lysis)
Detergent-based total protein extraction lyses cultured mammalian cells by using amphipathic detergents to disrupt lipid membranes and solubilize proteins into aqueous lysate; RIPA combines non-ionic detergent with ionic detergents, whereas NP-40/Triton buffers are milder non-ionic lysis conditions. The readout is the recovered clarified protein lysate, which is commonly quantified by detergent-compatible protein assays and then used for downstream protein analysis such as SDS-PAGE and Western blotting.
Reinheit & Dokumentation
Verweise
Calculators
Konzentration (Stammlösung) × Volumen (Stammlösung) = Konzentration (Ziellösung) × Volumen (Ziellösung)
Glucolimnanthin Related Classifications
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