h-FTAA
Based on 1 Customer Validation
h-FTAA is a luminescent conjugated oligothiophene amyloid-binding probe. h-FTAA binds pre-fibrillar amyloid-β (Amyloid-β) and AβArc aggregates, promotes fibril/protofibril formation, accelerates amyloid-β fibrillation, and reduces amyloid-β and AβArc-induced cytotoxicity and neurotoxicity-associated morphological changes in neuronal cells. h-FTAA recognizes the periphery of immature plaques, postmortem human brain plaque pathology, and all familial brain tissue Aβ deposits, labeling both plaque populations in a pan-amyloid binding manner. h-FTAA stains the corona of cored plaques and diffuse plaques, enabling multiplexed hyperspectral imaging. h-FTAA can be used for research on Alzheimer's disease.
Nur für Forschungszwecke. Wir verkaufen nicht an Patienten.
- Reinheit : 98.0%
- CAS. Nr.: 1352477-26-9
- Formel: C34H20O8S7
- Molecular Weight:780.97
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Speicherung:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biologische Aktivität
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Chemical Information
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Lösungsmittel & Löslichkeit
- Protokoll
- Reinheit & Dokumentation
- Verweise
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Help & FAQs
Biologische Aktivität
Beschreibung
In Vitro
h-FTAA (3 μM; 25 min) labels the diffuse immature peripheral regions of Aβ plaques in ex vivo brain sections from tgSwe and tgArcSwe mice[2].
h-FTAA (1.5 μM; 30 min) fluorescently labels Aβ plaques in postmortem PSEN1 fAD temporal cortex and validates MALDI-MSI plaque ROIs[3].
h-FTAA (200 nM; 30 min at room temperature) fully co-localizes with 4G8 and labels all Aβ deposits in PSEN1 A431E frontal cortex sections, including both the HS-276-positive and LL-1-positive plaque populations, with no laminar differences[4].
h-FTAA (3 μM; up to 3 h) attenuates Aβ1-42-induced toxicity in SH-SY5Y neuroblastoma cells upon co-aggregation[5].
h-FTAA (10 μM; aggregation 0-4 h; exposure 72 h) completely eliminates AβArc-mediated cytotoxicity in SH-SY5Y neuroblastoma cells, whereas 1 and 0.1 μM are inactive[5].
h-FTAA (10 μM; 0 h) prevents Aβ1-42- and AβArc-induced neurotoxic morphology in SH-SY5Y neuroblastoma cells[5].
h-FTAA (3-10 μM; Aβ1-42 3 h; AβArc 0 h) alters the morphology of Aβ1-42 and AβArc aggregates, among which AβArc forms protofibrils at 0 h[5].
h-FTAA (0.3 μM; 20 h) accelerates Aβ1-42 aggregation with a lag time of 1.39 h and a t0.5 of 2.47 h, whereas AβArc aggregation remains non-sigmoidal within 20 h[5].
h-FTAA (0.3 μM; 0-25 h) binds to Aβ1-42, shifting the excitation wavelength from 450 nm to 480 nm, and rapidly binds AβArc aggregates, reaching a binding plateau at 10 h[5].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:SH-SY5Y human neuroblastoma cells
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Concentration:3 μM (h-FTAA); 10 μM (Aβ1-42 aggregation); 3 μM (Aβ1-42 cell exposure)
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Incubation Time:1, 2, 3, 5 h (aggregation at 37°C); 72 h (cell exposure)
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Result:Co-aggregation increased SH-SY5Y viability relative to Aβ1-42 alone at the early aggregation times; the protective effect was reported for cell exposures after ≤ 3 h of Aβ1-42 aggregation.
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Cell Line:SH-SY5Y human neuroblastoma cells
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Concentration:0.1, 1, 10 μM (h-FTAA); 10 μM (AβArc aggregation); 3 μM (AβArc cell exposure)
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Incubation Time:1, 2, 4 h (aggregation at 37°C); 72 h (cell exposure)
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Result:At 10 μM, a protective effect was observed at all investigated times (1, and 2 h).
At an equimolar AβArc concentration, the cytotoxic effect was completely abolished.
Lower concentrations (0.1 and 1 μM) did not protect after any exposure period.
Chemical Information
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CAS. Nr. 1352477-26-9
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Appearance Solid
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Molecular Weight 780.97
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Formel C34H20O8S7
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Color Brown to reddish brown
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SMILES
O=C(C1=CC=C(C2=CC=C(C3=CC(CC(O)=O)=C(C4=CC=C(C5=C(CC(O)=O)C=C(C6=CC=C(C7=CC=C(C(O)=O)S7)S6)S5)S4)S3)S2)S1)O
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Versand
Room temperature in continental US; may vary elsewhere.
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Speicherung
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Lösungsmittel & Löslichkeit
In Vitro:
DMSO : 50 mg/mL (64.02 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Konzentration (Stammlösung) × Volumen (Stammlösung) = Konzentration (Ziellösung) × Volumen (Ziellösung)
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Working solution concentration: 0.22 mg/mL
Reinheit & Dokumentation
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Data Sheet (281 KB)
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SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
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Handling Instructions (2659 KB)
Verweise
[1]. Hanrieder J, et al. Isotope Encoded Spatial Biology Identifies Amyloid Plaque-Age-Dependent Structural Maturation, Synaptic Loss, and Increased Toxicity. [Content Brief]
[5]. Sandin L, et al. The Luminescent Conjugated Oligothiophene h‑FTAA Attenuates the Toxicity of Different Aβ Species. Biochemistry. 2021 Sep 21;60(37):2773-80. [Content Brief]
[6]. MALDI M. Multimodal Chemical Imaging to probe Alzheimer’s Disease Pathology.
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.2805 mL | 6.4023 mL | 12.8046 mL | 32.0115 mL |
| 5 mM | 0.2561 mL | 1.2805 mL | 2.5609 mL | 6.4023 mL | |
| 10 mM | 0.1280 mL | 0.6402 mL | 1.2805 mL | 3.2011 mL | |
| 15 mM | 0.0854 mL | 0.4268 mL | 0.8536 mL | 2.1341 mL | |
| 20 mM | 0.0640 mL | 0.3201 mL | 0.6402 mL | 1.6006 mL | |
| 25 mM | 0.0512 mL | 0.2561 mL | 0.5122 mL | 1.2805 mL | |
| 30 mM | 0.0427 mL | 0.2134 mL | 0.4268 mL | 1.0670 mL | |
| 40 mM | 0.0320 mL | 0.1601 mL | 0.3201 mL | 0.8003 mL | |
| 50 mM | 0.0256 mL | 0.1280 mL | 0.2561 mL | 0.6402 mL | |
| 60 mM | 0.0213 mL | 0.1067 mL | 0.2134 mL | 0.5335 mL |
h-FTAA Related Classifications
HY-177906 Related Classifications
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