ST1060
Based on 1 Customer Validation
ST1060 is a Ceramide synthase 2 (CerS2) inhibitor. ST1060 reduces the levels of long-chain dihydroceramide (dhCer) produced by CerS2 catalysis, with IC50 values of 63.6 μM and 61.3 μM for C24:0 and C24:1, respectively. ST1060 is applicable to the research of ceramide metabolism disorders and cervical cancer.
Nur für Forschungszwecke. Wir verkaufen nicht an Patienten.
- Reinheit : 96.90%
- CAS. Nr.: 1402702-76-4
- Formel: C35H63NO4
- Molecular Weight:561.88
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Speicherung:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biologische Aktivität
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Chemical Information
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Lösungsmittel & Löslichkeit
- Protokoll
- Reinheit & Dokumentation
- Verweise
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Help & FAQs
Biologische Aktivität
Beschreibung
In Vitro
ST1060 (preincubated for 10 min, reacted for 30 min) inhibits the synthesis of long-chain dihydroceramides (C24:0-dhCer, C24:1-dhCer) by targeting CerS2 in the microsomal fraction of HCT-116 cells, with IC50 values of 63.6 μM and 61.3 μM, respectively[1].
ST1060 (25 μM; pre-incubated for 10 minutes, reacted for 30 minutes) specifically inhibits CerS2 activity in lysates of HCT-116 cells overexpressing CerS2, as evidenced by a significant reduction in the production of C24:0 and C24:1-dhCer[1].
ST1060 (30 μM) significantly reduces the levels of long-chain dihydroceramides (C24:0-dhCer, C24:1-dhCer) in HeLa cervical cancer cells, confirming its targeted action on CerS2[1].
ST1060 (10-70 μM; 48 h) inhibits C14:0-ceramide synthesis in HeLa cervical cancer cells in a concentration-dependent manner, with an IC50 of 45.1 μM after 48 h of treatment, while increasing the level of long-chain ceramides[1].
ST1060 (0-80 μM; 48 h) increases the cell viability of HCT-116 colon cancer cells and induces their proliferation in a concentration-dependent manner following 48 h of treatment[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
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Cell Line:HCT-116 colon cancer cells
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Concentration:0-80 μM
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Incubation Time:48 h (ST1060 treatment); 90 min (WST-1 reagent incubation)
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Result:Increased HCT-116 cell viability in the tested concentration range.
Induced proliferation rate.
Showed no spontaneous necrosis/apoptosis in DMSO-treated control cells.
Chemical Information
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CAS. Nr. 1402702-76-4
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Appearance Solid
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Molecular Weight 561.88
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Formel C35H63NO4
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Color White to off-white
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SMILES
CCCCCCCOC1=CC=C(CCC(CO)(NC(CCCCCCCCCCCCCCCC)=O)CO)C=C1
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Versand
Room temperature in continental US; may vary elsewhere.
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Speicherung
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Lösungsmittel & Löslichkeit
In Vitro:
DMSO : 100 mg/mL (177.97 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Konzentration (Stammlösung) × Volumen (Stammlösung) = Konzentration (Ziellösung) × Volumen (Ziellösung)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (4.45 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (4.45 mM); Suspended solution
This protocol yields a suspended solution of ≥ 2.5 mg/mL (saturation unknown). Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Protokoll
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Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.
Reinheit & Dokumentation
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Data Sheet (269 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
Verweise
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 1.7797 mL | 8.8987 mL | 17.7974 mL | 44.4935 mL |
| 5 mM | 0.3559 mL | 1.7797 mL | 3.5595 mL | 8.8987 mL | |
| 10 mM | 0.1780 mL | 0.8899 mL | 1.7797 mL | 4.4493 mL | |
| 15 mM | 0.1186 mL | 0.5932 mL | 1.1865 mL | 2.9662 mL | |
| 20 mM | 0.0890 mL | 0.4449 mL | 0.8899 mL | 2.2247 mL | |
| 25 mM | 0.0712 mL | 0.3559 mL | 0.7119 mL | 1.7797 mL | |
| 30 mM | 0.0593 mL | 0.2966 mL | 0.5932 mL | 1.4831 mL | |
| 40 mM | 0.0445 mL | 0.2225 mL | 0.4449 mL | 1.1123 mL | |
| 50 mM | 0.0356 mL | 0.1780 mL | 0.3559 mL | 0.8899 mL | |
| 60 mM | 0.0297 mL | 0.1483 mL | 0.2966 mL | 0.7416 mL | |
| 80 mM | 0.0222 mL | 0.1112 mL | 0.2225 mL | 0.5562 mL | |
| 100 mM | 0.0178 mL | 0.0890 mL | 0.1780 mL | 0.4449 mL |
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