6-FAM

Guide (The following is our recommended solution. This solution is merely a guideline and should be modified according to your specific needs.)
In cell experiments, oligonucleotide probes labeled with 6-carboxyfluorescein (6-FAM) were used, mainly through the fluorescence in situ hybridization (FISH) technique [1]. (1) Sample preparation: Prepare chromosome samples of chicken embryonic cells, including steps such as colchicine treatment, hypotonic swelling, and methanol-acetic acid fixation.
(2) Pre-treatment: The chromosomes were treated with RNase A, pepsin and formaldehyde.
(3) Hybridization: The 6-FAM-labeled oligonucleotide was dissolved in the hybridization buffer containing Escherichia coli tRNA (50% formamide, 2×SSC, 10% dextran sulfate) at a concentration of 20 ng/μL. After the DNA and the probe were denatured together, they were hybridized at room temperature in a humid box overnight.
(4) Washing and re-staining: The hybridized slides were washed three times successively in 2×SSC, then dehydrated using a series of 70%-96% ethanol, dried, and sealed with an anti-quenching agent containing DAP I.
(5) Observation and Analysis: Use the Leica DM4000B fluorescence microscope equipped with a monochrome CCD camera DFC350FX and appropriate filters to observe the samples. Then, use the QFISH software to capture and process the colored images of the chromosomes.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.