Benfluorex
Based on 5 publication(s) in Google Scholar
Benfluorex (JP-992) is a hepatic nuclear factor 4 alpha (HNF4α) activator.
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- CAS No.: 23602-78-0
- Formule: C19H20F3NO2
- Masse moléculaire:351.36
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Stockage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Activité biologique
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Chemical Information
- Protocole
- Pureté et documentation
- Références
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) Benfluorex
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RT-PCR
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In Vivo Efficacy Study
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In Vivo Efficacy Study
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WB
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In Vivo Efficacy Study
Activité biologique
HNF4α[1]
Benfluorex consistently activates insulin promoter activity as measured by an increased number of GFP-positive cells. Benfluorex increases the number of GFP-positive cells in a dose-responsive manner and increases the level of endogenous insulin mRNA. Consistent with being HNF4α activator, Benfluorex stimulates HNF4α expression. Benfluorex alters HNF4α protease sensitivity, while the inactive control compound does not[1]. Benfluorex decreases, in a concentration-dependent manner, the synthesis of acid-soluble products and ketone bodies from oleate, whereas the production of 14CO2 into citric acid cycle is markedly increased by Benfluorex. Benfluorex inhibits in a dose-dependent manner the rates of gluconeogenesis from lactate/pyruvate (10/1 nM)[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 23602-78-0
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Masse moléculaire 351.36
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Formule C19H20F3NO2
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SMILES
FC(C1=CC(CC(NCCOC(C2=CC=CC=C2)=O)C)=CC=C1)(F)F
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Synonyms
JP-992
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
Please store the product under the recommended conditions in the Certificate of Analysis.
Publications (5)
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Journal Impact Factor
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Most Recent
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Adv Sci (Weinh)
Short-Term Statin Therapy Induces Hepatic Insulin Resistance Through HNF4α/PAQR9/PPM1α Axis Regulated AKT Phosphorylation. [Abstract]2024 Jul 5:e2403451. PMID: 38970167
Benfluorex purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2024 Jul 5:e2403451. [Abstract]
Relative mRNA levels of Paqr9, Ulk1, Cyp7a1 and Lipc in mouse primary hepatocytes with atorvastatin (Ator) and different concentration of Benfluorex hydrochloride (BFX) (2-50 μM).
Benfluorex purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2024 Jul 5:e2403451. [Abstract]
Benfluorex hydrochloride (B) (20 mg/kg; i.g.; every other day for 3 weeks) improved levels of fasting glucose affected by atorvastatin treatment in Paqr9-/- mice.
Benfluorex purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2024 Jul 5:e2403451. [Abstract]
Benfluorex hydrochloride (B) (20 mg/kg; i.g.; every other day for 3 weeks) improved levels of insulin and FFA affected by atorvastatin treatment in Paqr9-/- mice.
Benfluorex purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2024 Jul 5:e2403451. [Abstract]
Benfluorex hydrochloride (B) (20 mg/kg; i.g.; every other day for 3 weeks) increased hepatic AKT1 phosphorylation and lowered the PAQR9 levels raised by atorvastatin in Paqr9-/- mice.
Benfluorex purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2024 Jul 5:e2403451. [Abstract]
Benfluorex hydrochloride (B) (20 mg/kg; i.g.; every other day for 3 weeks) alleviated glucose tolerance and insulin tolerance of Paqr9-/- mice.
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EBioMedicine
Intestinal epithelial β Klotho is a critical protective factor in alcohol-induced intestinal barrier dysfunction and liver injury. [Abstract]2022 Aug:82:104181. PMID: 35908416 -
Biochem Pharmacol
AMPKα2/HNF4A/BORIS/GLUT4 pathway promotes hepatocellular carcinoma cell invasion and metastasis in low glucose microenviroment. [Abstract]2022 Sep:203:115198. PMID: 35940258 -
Virol J
GATA binding protein 4 promotes the expression and transcription of hepatitis B virus by facilitating hepatocyte nuclear factor 4 alpha in vitro. [Abstract]2021 Sep 28;18(1):196. PMID: 34583732 -
Protocole
HepG2 cells are treated with DMSO or Benfluorex at a concentration of 20 µM or 40 µM for 16 hr. Total cell protein is extracted, measured by BCA protein assay. Each sample is split into two aliquots for proteolysis without (-) or with (+) Subtilisin. Twenty ug of cell lysate is incubated with or without protease (20 ng/mL subtilisin) for 35 minutes at room temperature. Western blot is then performed with primary anti-HNF4α polyclonal antibody (1:1000 dilution) and secondary HRP conjugated anti-goat IgG (1:2000 dilution), detected with chemiluminescence ECL kit[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Hepatocytes are isolated by in situ perfusion of the liver with 0.025% collagenase. Hepatocytes (1 to 2×106 cells/mL) are incubated at 37°C in 2 mL of oxygenated (O2:CO2; 95:5) Krebs-Henseleit bicarbonate buffer (pH 7.4) for 1 h in a gyratory shaking water bath. Benfluorex is dissolved in DMSO and added (10 μL) to the incubation medium at a final concentration of 0.1 or 1 nM[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Pureté et documentation
Références
[1]. Lee SH, et al. Identification of alverine and benfluorex as HNF4α activators. ACS Chem Biol. 2013 Aug 16;8(8):1730-6. [Content Brief]
[2]. Kohl C, et al. Effects of benfluorex on fatty acid and glucose metabolism in isolated rat hepatocytes: from metabolic fluxes to gene expression. Diabetes. 2002 Aug;51(8):2363-8. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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