KKL-35
Based on 1 Customer Validation
KKL-35 is a trans-translation tagging reaction inhibitor with an IC50 of 0.9 μM.
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- Pureté: 99.46%
- CAS No.: 865285-29-6
- Formule: C15H9ClFN3O2
- Masse moléculaire:317.70
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Stockage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Activité biologique
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Chemical Information
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Solvant et solubilité
- Pureté et documentation
- Références
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Help & FAQs
Activité biologique
IC50: 0.9 μM (trans-translation tagging reaction)[1]
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| Caco-2 | IC50 |
14 μM
Compound: KKL-35
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Cytotoxicity against human Caco2 cells assessed as decrease in cell viability in MTT assay
Cytotoxicity against human Caco2 cells assessed as decrease in cell viability in MTT assay
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[PMID: 31540826] |
| HCT-116 | IC50 |
17 μM
Compound: KKL-35
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Cytotoxicity against human HCT116 cells assessed as decrease in cell viability in MTT assay
Cytotoxicity against human HCT116 cells assessed as decrease in cell viability in MTT assay
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[PMID: 31540826] |
| HEK293 | IC50 |
8.9 μM
Compound: 42
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Inhibition of human AC1 expressed in HEK293 cells assessed as decrease in A23187-stimulated cAMP accumulation preincubated for 30 mins followed by A23187 stimulation and measured after 1 hr by fluorescence assay
Inhibition of human AC1 expressed in HEK293 cells assessed as decrease in A23187-stimulated cAMP accumulation preincubated for 30 mins followed by A23187 stimulation and measured after 1 hr by fluorescence assay
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[PMID: 30472604] |
| Huh-7 | IC50 |
20 μM
Compound: KKL-35
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Cytotoxicity against human HuH7 cells assessed as decrease in cell viability in MTT assay
Cytotoxicity against human HuH7 cells assessed as decrease in cell viability in MTT assay
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[PMID: 31540826] |
| MCF7 | IC50 |
22 μM
Compound: KKL-35
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Cytotoxicity against human MCF7 cells assessed as decrease in cell viability in MTT assay
Cytotoxicity against human MCF7 cells assessed as decrease in cell viability in MTT assay
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[PMID: 31540826] |
| MDA-MB-231 | IC50 |
25 μM
Compound: KKL-35
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Cytotoxicity against human MDA-MB-231 cells assessed as decrease in cell viability in MTT assay
Cytotoxicity against human MDA-MB-231 cells assessed as decrease in cell viability in MTT assay
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[PMID: 31540826] |
| NCI-H727 | IC50 |
18 μM
Compound: KKL-35
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Cytotoxicity against human NCI-H727 cells assessed as decrease in cell viability in MTT assay
Cytotoxicity against human NCI-H727 cells assessed as decrease in cell viability in MTT assay
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[PMID: 31540826] |
| PC-3 | IC50 |
21 μM
Compound: KKL-35
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Cytotoxicity against human PC3 cells assessed as decrease in cell viability in MTT assay
Cytotoxicity against human PC3 cells assessed as decrease in cell viability in MTT assay
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[PMID: 31540826] |
KKL-35 exhibits broad-spectrum antibiotic activity. KKL-35 prevents growth of B. anthracis and M. smegmatis with minimum inhibitory concentration (MIC) values of less than 6 μM. KKL-35 inhibit trans-translation at some step before proteolysis of tagged proteins. KKL-35 inhibits tagging of DHFR-ns. A large amount of untagged DHFR is produced in reactions with the highest concentrations of KKL-35, indicating that KKL-35 does not inhibit translation. KKL-35 prevents growth of WT S. flexneri with a MIC of 6 μM, and addition of KKL-35 to a growing culture of S. flexneri stops growth. In an S. flexneri strain expressing ArfA and deleted for ssrA, addition of KKL-35 has little effect on viability or growth rate. KKL-35 inhibits the growth of E. coli tolC, which is deficient in small molecule efflux, with an MIC of 0.3 μM[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 865285-29-6
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Appearance Solid
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Masse moléculaire 317.70
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Formule C15H9ClFN3O2
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Color White to light yellow
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SMILES
O=C(NC1=NN=C(C2=CC=C(F)C=C2)O1)C3=CC=C(Cl)C=C3
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvant et solubilité
DMSO : 6.25 mg/mL (19.67 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Pureté et documentation
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Fiche technique (282 KB)
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SDS (392 KB)
- English - EN (392 KB)
- Français - FR (392 KB)
- Deutsch - DE (392 KB)
- Norwegian - NO (392 KB)
- Español - ES (392 KB)
- Swedish - SV (392 KB)
- Italian - IT (392 KB)
- Korean - KR (392 KB)
- Portuguese - PT (392 KB)
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Instruction de manipulation (2659 KB)
Références
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.1476 mL | 15.7381 mL | 31.4762 mL | 78.6906 mL |
| 5 mM | 0.6295 mL | 3.1476 mL | 6.2952 mL | 15.7381 mL | |
| 10 mM | 0.3148 mL | 1.5738 mL | 3.1476 mL | 7.8691 mL | |
| 15 mM | 0.2098 mL | 1.0492 mL | 2.0984 mL | 5.2460 mL |
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