Biotin-cholesterol
Based on 1 Customer Validation
Biotin-cholesterol is a biotinylated form of Cholesterol (HY-N0322). Biotin-cholesterol is a liver-targeting ligand (linking cholesterol and biotin via a Gly-Lys dipeptide linker), as well as an enhancer of the component activity of streptavidin-mediated siRNA nanocomplexes. Biotin-cholesterol promotes receptor-mediated endocytosis of the relevant siRNA nanocomplexes, protects siRNA from serum degradation, enables efficient siRNA delivery, and drives target gene silencing. Biotin-cholesterol shows no cytotoxicity in hepatic stellate cells. Biotin-cholesterol can be used in the research of alcoholic liver fibrosis.
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- Pureté : 97.2%
- CAS No.: 2454045-15-7
- Formule: C37H60N2O3S
- Masse moléculaire:612.95
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Stockage:
4°C, protect from light, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Activité biologique
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Chemical Information
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Solvant et solubilité
- Protocole
- Pureté et documentation
- Références
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Help & FAQs
Activité biologique
Description
In Vitro
Biotin-cholesterol forms stable SSC complexes with biotin-siRNA and streptavidin at an optimized 2:1:2 molar ratio of siRNA:streptavidin:cholesterol, demonstrating comparable streptavidin binding affinity to biotin-siRNA[1].
Biotin-cholesterol (3-6 h) enhances cellular uptake of SSCP nanocomplexes in HSC-T6 rat hepatic stellate cells[1].
Biotin-cholesterol (150 μg/mL puromycin pre-treatment; 1 h) mediates LDLR-dependent cellular uptake of SSCP nanocomplexes, resulting in higher uptake in HSC-T6 rat hepatic stellate cells than in PC-3 or CaCo-2 cells due to higher LDLR expression in HSC-T6 cells[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
- Fibrosis/Collagen Morphometry
- Collagen: Sirius Red Staining
- Cell Cytotoxicity Assay
- Mammalian live/dead viability and cytotoxicity staining
- Connective Tissue: Masson's Trichrome/Collagen Trichrome Staining
- RNA interference technology
- Cell Viability Determination by MTT Colorimetric Assay
- Liver Histomorphometry
Chemical Information
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CAS No. 2454045-15-7
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Appearance Solid
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Masse moléculaire 612.95
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Formule C37H60N2O3S
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Color White to off-white
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SMILES
C[C@@]12[C@]3([H])[C@](CC=C1C[C@H](CC2)OC(CCCC[C@H]4[C@]5([H])[C@](NC(N5)=O)([H])CS4)=O)([H])[C@@]6([H])[C@](CC3)([C@@](CC6)([H])[C@H](C)CCCC(C)C)C
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
4°C, protect from light, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
Solvant et solubilité
In Vitro:
Ethanol : 0.5 mg/mL (0.82 mM; ultrasonic and warming and heat to 60°C)
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Working solution concentration: 0.22 mg/mL
Pureté et documentation
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Fiche technique (273 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Instruction de manipulation (2659 KB)
Références
Biotin-cholesterol Related Classifications
HY-156197 Related Classifications
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