CDg13
CDg13 is a fluorescent probe that specifically detects living neural stem/progenitor cells. CDg13 localizes to the endoplasmic reticulum via its dihexyl moiety, with no interaction with endoplasmic reticulum biomolecules. CDg13 undergoes selective efflux by active ABCG2 transporters, with increased intracellular accumulation following ABCG2 inhibition or knockdown. CDg13 isolates and enriches self-renewable neural stem/progenitor cells from embryonic mouse brain tissue, stains neural stem/progenitor cells brightly, and sorts ABCG2low cell populations from heterogeneous populations. (Ex/Em = 520/553 nm)
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- CAS No.: 1451048-96-6
- Formule: C32H35Cl2NO4
- Masse moléculaire:568.53
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Stockage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Activité biologique
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Chemical Information
- Pureté et documentation
- Références
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Help & FAQs
Activité biologique
CDg13 (1 μM; 1 h) selectively stains live mouse NS5 (NSPC) cells with bright fluorescence, while showing minimal staining of MEF, mESC, and differentiated NS5 cells[1].
CDg13 (1 μM; 1 h) fluorescence in D-NS5 cells is inversely correlated with Abcg2 expression, as demonstrated by enhanced fluorescence in Abcg2-knockdown cells stained with it[1].
CDg13 (1-10 μM; 48 h-7days) has very low toxicity to live mouse NSPCs[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:mouse embryonic fibroblasts (MEF), mouse embryonic stem cells (mESC), mouse NSPC line (NS5), differentiated NS5 (D-NS5)
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Concentration:1 μM
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Incubation Time:1 h
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Result:Showed bright fluorescence in NS5 cells, with significantly lower fluorescence in MEF, mESC, and D-NS5 cells, as visualized by fluorescence microscopy and confirmed by flow cytometry histograms.
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Cell Line:mouse NSPCs (NS5 cells)
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Concentration:1, 10 μM
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Incubation Time:48 h; 7 days;
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Result:Showed no cell toxicity after incubation with 10 μM for 48 h.
Showed no effect on neurosphere size after incubation with 1 μM for 7 days.
Chemical Information
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CAS No. 1451048-96-6
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Masse moléculaire 568.53
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Formule C32H35Cl2NO4
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SMILES
CCCCCCN(C(C1=CC=C(C=C1)C2=C3C=C(C(C=C3OC4=C2C=C(C(O)=C4)Cl)=O)Cl)=O)CCCCCC
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
Please store the product under the recommended conditions in the Certificate of Analysis.
Pureté et documentation
Références
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
CDg13 Related Classifications
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