Cat. No.: HY-N6037

Gardenin B

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Gardenin B is a methoxyflavone compound and an inhibitor of USP7, ODC (IC₅₀: 6.24 μg/mL), and Cathepsin D (IC₅₀: 5.61 μg/mL). Gardenin B exhibits antioxidant and antitumor activities. Gardenin B shows IC₅₀ values of 8.87 and 10.59 μg/mL for DPPH and NO scavenging, respectively, and also possesses ferric ion reducing ability. Additionally, Gardenin B can inhibit tumor cell proliferation, induce cell cycle arrest and apoptosis. Gardenin B can be used in cancer research.

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Pureté: 99.69%
CAS No.: 2798-20-1
Formule: C₁₉H₁₈O₇
Masse moléculaire:358.34

Stockage:

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Gardenin B Chemical Structure

CAS No.: 2798-20-1

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Activité biologique
- Description
- Cellular Effect
- In Vitro
Chemical Information
Solvant et solubilité
- In Vitro
- In Vivo
Pureté et documentation
Références
Help & FAQs
Classifications
Bioz

Activité biologique

Description

Voir tous les produits spécifiques à Isoform Deubiquitinase :

Voir tous les produits spécifiques à Isoform Cathepsin :

Voir tous les produits spécifiques à Isoform Caspase :

Cellular Effect

Cell Line	Type	Value	Description	References
HepG2	IC₅₀	40 μM Compound: 5	Antiproliferative activity against human HepG2 cells after 48 hrs by MTT assay Antiproliferative activity against human HepG2 cells after 48 hrs by MTT assay	[PMID: 23800391]
HL-60	IC₅₀	>100 μM Compound: X, 5-demethyltangeretin	Antiproliferative activity against HL60 after 24 hrs Antiproliferative activity against HL60 after 24 hrs	[PMID: 17391969]
KB	ED₅₀	6 μg/mL Compound: 3, 5-O-desmethyltangeretin	Cytotoxicity against human KB cells Cytotoxicity against human KB cells	[PMID: 521819]
MCF7	IC₅₀	30 μM Compound: 5	Antiproliferative activity against human MCF7 cells after 48 hrs by MTT assay Antiproliferative activity against human MCF7 cells after 48 hrs by MTT assay	[PMID: 23800391]
Vero	IC₅₀	85 μM Compound: 5	Antiproliferative activity against african green monkey Vero cells after 48 hrs by MTT assay Antiproliferative activity against african green monkey Vero cells after 48 hrs by MTT assay	[PMID: 23800391]
WI-38	IC₅₀	75 μM Compound: 5	Antiproliferative activity against human WI38 cells after 48 hrs by MTT assay Antiproliferative activity against human WI38 cells after 48 hrs by MTT assay	[PMID: 23800391]

In Vitro

Gardenin B (0-100 μM; 0-72 h) inhibits the growth of leukemia cell lines (with IC₅₀ values of 1.6 and 3.0 μM for HL-60 and U-937, respectively), and induces cell cycle arrest, apoptosis, and production of ROS^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

Cell Line:

HL-60 and U-937 cells
Concentration:

0, 0.3, 1, 3, 10, 30 and 100 μM
Incubation Time:

72 h
Result:

Inhibited the cell viability.

Cell Viability Assay^[1]

Cell Line:

HL-60 and U-937 cells
Concentration:

0, 3 and 10 μM
Incubation Time:

24 h
Result:

Reduced the levels of Procaspase-2, -8, -9 and -3.
Inhibited the levels of Bcl-2 and PARP.

Chemical Information

CAS No. 2798-20-1
Appearance Solid
Masse moléculaire 358.34
Formule C₁₉H₁₈O₇
Color Light yellow to green yellow
SMILES

OC1=C2C(OC(C3=CC=C(OC)C=C3)=CC2=O)=C(OC)C(OC)=C1OC
Structure Classification
- Flavonoids
- Flavones
- Phenols
- Monophenols
Initial Source
Livraison

Room temperature in continental US; may vary elsewhere.
Stockage

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvant et solubilité

In Vitro:

DMSO : 8.33 mg/mL (23.25 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.7906 mL

13.9532 mL

27.9065 mL

5 mM

0.5581 mL

2.7906 mL

5.5813 mL

10 mM

0.2791 mL

1.3953 mL

2.7906 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Calculateur de molarité

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Calculateur de dilution

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: 2.5 mg/mL (6.98 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: 2.5 mg/mL (6.98 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Pureté et documentation

Select Batch:

Pureté: 99.98%

Assay: 99.98%

ee.: 99.98%

Fiche technique (275 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Instruction de manipulation (2659 KB)

Références

[1]. Cabrera J, et al. Gardenin B-induced cell death in human leukemia cells involves multiple caspases but is independent of the generation of reactive oxygen species. Chem Biol Interact. 2016 Aug 25;256:220-7. [Content Brief]

[2]. Zhang S, et al. Gardenin B, A Natural Inhibitor for USP7: In vitro Evaluation and In silico Identification. Letters in Drug Design & Discovery, 2024, 21(12): 2352-2358.

[3]. Maurya P, et al. Characterization of bioactive constituents from the gum resin of Gardenia lucida and its pharmacological potential. Biomed Pharmacother. 2017 Jan;85:444-456. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.7906 mL	13.9532 mL	27.9065 mL	69.7661 mL
	5 mM	0.5581 mL	2.7906 mL	5.5813 mL	13.9532 mL
	10 mM	0.2791 mL	1.3953 mL	2.7906 mL	6.9766 mL
	15 mM	0.1860 mL	0.9302 mL	1.8604 mL	4.6511 mL
	20 mM	0.1395 mL	0.6977 mL	1.3953 mL	3.4883 mL

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Gardenin B

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Voir tous les produits spécifiques à Isoform Deubiquitinase

Voir tous les produits spécifiques à Isoform Cathepsin

Voir tous les produits spécifiques à Isoform Caspase

Activité biologique

Chemical Information

Solvant et solubilité

Pureté et documentation

Références

Help & FAQs

Complete Stock Solution Preparation Table

Gardenin B Related Classifications

HY-N6037 Related Classifications