MAPTAM
MAPTAM is an intracellular Ca2+ chelator.
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- CAS No.: 147504-94-7
- Formule: C36H44N2O18
- Masse moléculaire:792.74
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Stockage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Activité biologique
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Chemical Information
- Protocole
- Pureté et documentation
- Références
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Help & FAQs
Activité biologique
Description
In Vitro
Guidelines[1] (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
Labeling of Cells:
1. Incubate the cells according to your normal protocol.
2. Pre-incubate cells for 30 minutes with 20 μM MAPTAM.
3. Replace medium with HEPES buffer containing 2 μM fura 2 pentaacetoxymethyl ester and 1% BSA (wt:vol).
4. Perform measurements in HEPES buffer containing 1 mM CaCl2.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
Chemical Information
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CAS No. 147504-94-7
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Masse moléculaire 792.74
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Formule C36H44N2O18
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SMILES
CC1=CC=C(N(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O)C(OCCOC2=CC(C)=CC=C2N(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O)=C1
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocole
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Ca2+ Staining Technique
Ca2+ staining is an experimental technique that utilizes specific fluorescent probes (such as Fluo-4 AM, Fura-2, etc.) to qualitatively or quantitatively detect dynamic changes in intracellular Ca2+ concentrations; this is achieved by monitoring the changes in fluorescent signals generated when these probes bind to free intracellular calcium ions. The underlying principle relies primarily on the presence of chelating groups within the probe's molecular structure that possess high affinity for calcium ions.
Pureté et documentation
Références
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
MAPTAM Related Classifications
HY-135433 Related Classifications
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