ML-184
Based on 1 Customer Validation
ML-184 (CID244033) is a selective GPR55 agonist with an EC50 of 250 nM, more than 100-fold selectivity for GPR55 over GPR35, CB1, and CB2. ML-184 induces ERK1/2 phosphorylation and PKCβII translocation to the plasma membrane via activation of GPR55. ML-184 (CID2440433) increases the proliferation of neural stem cells and promotes neuronal differentiation in vitro.
Nos produits utilisent uniquement pour la recherche. Nous ne vendons pas aux patients.
- Pureté: 99.57%
- CAS No.: 794572-10-4
- Formule: C25H34N4O3S
- Masse moléculaire:470.63
-
Stockage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Activité biologique
-
Chemical Information
-
Solvant et solubilité
- Pureté et documentation
- Références
-
Help & FAQs
-
GPCR/G Protein Compound Library
HY-L006
-
Neuronal Signaling Compound Library
HY-L013
-
Membrane Protein-targeted Compound Library
HY-L149
-
Membrane Receptor-targeted Compound Library
HY-L150
-
Highly Selective Activators Library
HY-L159
-
Bioactive Compound Library Max
HY-L181
-
MCE Bioactive Compound Library
HY-L001V
-
Bioactive Compound Library
HY-L001
-
High-Throughput Bioactive Compound Library
HY-L205
-
Lactylation Compound Library
HY-L249
Activité biologique
ML-184 (1 μM; 48 h) increases the proportion of cells in the S phase and promotes cell proliferation in human neural stem cells (hNSCs), an effect that could be blocked by pretreatment with 5 μM ML193[2].
ML-184 (1 μM; 10 d) significantly increases the mRNA and protein expressions of neuronal markers βIII-tubulin and microtubule-associated protein 2 (MAP2) in hNSCs, without significant effect on the astrocyte marker S100β[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
-
Cell Line:Human neural stem cells (hNSCs)
-
Concentration:1 μM
-
Incubation Time:48 h
-
Result:Increased the percentage of BrdU-positive cells in S phase and promoted cell proliferation.
Attenuated by co-treatment with the GPR55 antagonist ML193 (5 µM).
Flow cytometric analysis showed a significant shift of cells from G0/G1 to S phase, indicating enhanced proliferation.
-
Cell Line:Human neural stem cells (hNSCs)
-
Concentration:1 μM
-
Incubation Time:10 d
-
Result:Induced neuronal differentiation, as evidenced by increased immunofluorescence staining for βIII-tubulin and upregulated mRNA expression of βIII-tubulin and MAP2.
-
Cell Line:Human neural stem cells (hNSCs)
-
Concentration:1 μM
-
Incubation Time:10 d
-
Result:Resulted a 1.5- to 2-fold increase in neuronal marker genes compared to vehicle control, with no significant changes in glial marker S100β.
ML-184 can increase β-cell area and β-cell mass and reduce α-cell area and mass in high-fat-fed (HFF) mice. ML-184 or combined with Sitagliptin (HY-13749) can significantly increase circulating GIP levels and reduce food intake in mice; it can also increase jejunal GPR55 and JNK1 gene expression levels[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
-
CAS No. 794572-10-4
-
Appearance Solid
-
Masse moléculaire 470.63
-
Formule C25H34N4O3S
-
Color White to off-white
-
SMILES
CC1=CC=CC(N2CCN(CC2)C(C3=CC(S(N(C)C)(=O)=O)=CC=C3N4CCCC4)=O)=C1C
-
Synonyms
CID2440433
-
Livraison
Room temperature in continental US; may vary elsewhere.
-
Stockage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvant et solubilité
DMSO : 12.5 mg/mL (26.56 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Pureté et documentation
-
Fiche technique (279 KB)
-
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
-
Instruction de manipulation (2659 KB)
Références
[1]. Kotsikorou E, et al. Identification of the GPR55 agonist binding site using a novel set of high-potency GPR55 selective ligands. Biochemistry. 2011 Jun 28;50(25):5633-47. [Content Brief]
[2]. Hill JD, et al. Activation of GPR55 increases neural stem cell proliferation and promotes early adult hippocampal neurogenesis. Br J Pharmacol. 2018 Aug;175(16):3407-3421. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.1248 mL | 10.6241 mL | 21.2481 mL | 53.1203 mL |
| 5 mM | 0.4250 mL | 2.1248 mL | 4.2496 mL | 10.6241 mL | |
| 10 mM | 0.2125 mL | 1.0624 mL | 2.1248 mL | 5.3120 mL | |
| 15 mM | 0.1417 mL | 0.7083 mL | 1.4165 mL | 3.5414 mL | |
| 20 mM | 0.1062 mL | 0.5312 mL | 1.0624 mL | 2.6560 mL | |
| 25 mM | 0.0850 mL | 0.4250 mL | 0.8499 mL | 2.1248 mL |
ML-184 Related Classifications
HY-116461 Related Classifications
Powered by Bioz