NPBA
Based on 1 Customer Validation
NPBA, a potassium K2P channel TASK-3 (KCNK9) agonist, is a tandem pore domain weak inward rectifying K+ channel (TWIK2) channel blocker. NPBA suppresses NLRP3 inflammasome activation in macrophages.
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- Pureté: 99.56%
- CAS No.: 524033-40-7
- Formule: C16H14F3N3O3
- Masse moléculaire:353.30
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Stockage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Activité biologique
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Chemical Information
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Solvant et solubilité
- Pureté et documentation
- Références
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Help & FAQs
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Mechanoreceptors Compound Library
HY-L095
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Antidepressant Compound Library
HY-L108
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Potassium Channel Compound Library
HY-L119
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Membrane Protein-targeted Compound Library
HY-L149
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Membrane Receptor-targeted Compound Library
HY-L150
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Ion Channel Compound Library
HY-L166
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Macrophage Related Compound Library
HY-L174
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Inflammasomes related Compound Library
HY-L175
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Bioactive Compound Library Max
HY-L181
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MCE Bioactive Compound Library
HY-L001V
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Bioactive Compound Library
HY-L001
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High-Throughput Bioactive Compound Library
HY-L205
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Immunology/Inflammation Compound Library
HY-L007
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Membrane Transporter/Ion Channel Compound Library
HY-L011
Activité biologique
Chemical Information
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CAS No. 524033-40-7
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Appearance Solid
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Masse moléculaire 353.30
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Formule C16H14F3N3O3
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Color Light yellow to green yellow
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SMILES
O=C(C1=CC=CC=C1)NCCNC2=CC=C([N+]([O-])=O)C=C2C(F)(F)F
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvant et solubilité
DMSO : 100 mg/mL (283.05 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (7.08 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Pureté et documentation
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Fiche technique (270 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Instruction de manipulation (2659 KB)
Références
[1]. Fuyun Tian, et al. A Small-Molecule Compound Selectively Activates K2P Channel TASK-3 by Acting at Two Distant Clusters of Residues. Mol Pharmacol. 2019 Jul;96(1):26-35. [Content Brief]
[2]. Yuanxing Zhi, et al. A novel TWIK2 channel inhibitor binds at the bottom of the selectivity filter and protects against LPS-induced experimental endotoxemia in vivo. Biochem Pharmacol. 2023 Dec:218:115894. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.8305 mL | 14.1523 mL | 28.3046 mL | 70.7614 mL |
| 5 mM | 0.5661 mL | 2.8305 mL | 5.6609 mL | 14.1523 mL | |
| 10 mM | 0.2830 mL | 1.4152 mL | 2.8305 mL | 7.0761 mL | |
| 15 mM | 0.1887 mL | 0.9435 mL | 1.8870 mL | 4.7174 mL | |
| 20 mM | 0.1415 mL | 0.7076 mL | 1.4152 mL | 3.5381 mL | |
| 25 mM | 0.1132 mL | 0.5661 mL | 1.1322 mL | 2.8305 mL | |
| 30 mM | 0.0943 mL | 0.4717 mL | 0.9435 mL | 2.3587 mL | |
| 40 mM | 0.0708 mL | 0.3538 mL | 0.7076 mL | 1.7690 mL | |
| 50 mM | 0.0566 mL | 0.2830 mL | 0.5661 mL | 1.4152 mL | |
| 60 mM | 0.0472 mL | 0.2359 mL | 0.4717 mL | 1.1794 mL | |
| 80 mM | 0.0354 mL | 0.1769 mL | 0.3538 mL | 0.8845 mL | |
| 100 mM | 0.0283 mL | 0.1415 mL | 0.2830 mL | 0.7076 mL |
NPBA Related Classifications
HY-139064 Related Classifications
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